Examining the efficacy of mushroom industry biocides on
            <i>Listeria monocytogenes</i>
            biofilm

Dygico, Lionel Kenneth; Gahan, Cormac G. M.; Grogan, Helen; Burgess, Catherine M.

doi:10.1111/jam.14681

Cited by 5 publications

(2 citation statements)

References 49 publications

(64 reference statements)

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“…It is an opportunistic pathogen with a high fatality rate in susceptible populations. ( Dygico et al, 2021 ) It can grow under adverse conditions in food production environment. Therefore, it is important to improve the detection efficiency of L. monocytogenes .…”

Section: Resultsmentioning

confidence: 99%

Fast Broad-Spectrum Staining and Photodynamic Inhibition of Pathogenic Microorganisms by a Water-Soluble Aggregation-Induced Emission Photosensitizer

et al. 2021

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Pathogenic microorganisms pose great challenges to public health, which is constantly urgent to develop extra strategies for the fast staining and efficient treatments. In addition, once bacteria form stubborn biofilm, extracellular polymeric substance (EPS) within biofilm can act as protective barriers to prevent external damage and inward diffusion of traditional antibiotics, which makes it frequently develop drug-resistant ones and even hard to treat. Therefore, it is imperative to develop more efficient methods for the imaging/detection and efficient inhibition of pathogenic microorganisms. Here, a water-soluble aggregation-induced emission (AIE)-active photosensitizer TPA-PyOH was employed for fast imaging and photodynamic treatment of several typical pathogens, such as S. aureus, methicillin-resistant Staphylococcus aureus, L. monocytogenes, C. albicans, and E. coli. TPA-PyOH was non-fluorescent in water, upon incubation with pathogen, positively charged TPA-PyOH rapidly adhered to pathogenic membrane, thus the molecular motion of TPA-PyOH was restricted to exhibit AIE-active fluorescence for turn-on imaging with minimal background. Upon further white light irradiation, efficient reactive oxygen species (ROS) was in-situ generated to damage the membrane and inhibit the pathogen eventually. Furthermore, S. aureus biofilm could be suppressed in vitro. Thus, water-soluble TPA-PyOH was a potent AIE-active photosensitizer for fast fluorescent imaging with minimal background and photodynamic inhibition of pathogenic microorganisms.

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Section: Resultsmentioning

confidence: 99%

Fast Broad-Spectrum Staining and Photodynamic Inhibition of Pathogenic Microorganisms by a Water-Soluble Aggregation-Induced Emission Photosensitizer

et al. 2021

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“…However, most of them are either not applicable to in-line monitoring of biofilm formation or do not provide the results in real time. Conventional methods for detecting bacterial pathogens in biofilm are still based on culturing the microorganisms on agar plates after swabbing biological material from the food contact surface [130]. In order to overcome the problem with bacteria in viable but nonculturable states, direct epifluorescence microscopy, enzyme-linked immunosorbent assays, or PCR have been proposed as more sensitive, albeit more expensive and timeconsuming, methods for the detection of pathogens in biofilm [131].…”

Section: Detection Of Biofilm In Food Processing Plantsmentioning

confidence: 99%

Electrochemical Control of Biofilm Formation and Approaches to Biofilm Removal

et al. 2022

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This review deals with microbial adhesion to metal-based surfaces and the subsequent biofilm formation, showing that both processes are a serious problem in the food industry, where pathogenic microorganisms released from the biofilm structure may pollute food and related material during their production. Biofilm exhibits an increased resistance toward sanitizers and disinfectants, which complicates the removal or inactivation of microorganisms in these products. In the existing traditional techniques and modern approaches for clean-in-place, electrochemical biofilm control offers promising technology, where surface properties or the reactions taking place on the surface are controlled to delay or prevent cell attachment or to remove microbial cells from the surface. In this overview, biofilm characterization, the classification of bacteria-forming biofilms, the influence of environmental conditions for bacterial attachment to material surfaces, and the evaluation of the role of biofilm morphology are described in detail. Health aspects, biofilm control methods in the food industry, and conventional approaches to biofilm removal are included as well, in order to consider the possibilities and limitations of various electrochemical approaches to biofilm control with respect to potential applications in the food industry.

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