Examining the effect of the CaMKII inhibitor administration in the locus coeruleus on the naloxone-precipitated morphine withdrawal signs in rats

Navidhamidi, Mojdeh; Semnanian, Saeed; Javan, Mohammad; Goudarzvand, Mahdi; Rohampour, Kambiz; Azizi, Hossein

doi:10.1016/j.bbr.2011.09.043

Cited by 6 publications

(2 citation statements)

References 29 publications

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“…Evidence has shown that CaMKII inhibitors suppress the development and formation of tolerance to morphine when selectively administered to rats’ brain (Fan, Wang, Qiu, Ma & Pei, 1999 ). Furthermore, inhibition of CaMKIIα in the locus coeruleus significantly attenuates some signs of morphine withdrawal (Navidhamidi et al, 2012 ). It has also been demonstrated that CaMKII activity in the NAc shell is essential for relapse in morphine-seeking behaviors (Liu, Liu, Zhang & Yu, 2012 ; Liu, Zhang, Liu & Yu, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Gene Expression Profile of Calcium/Calmodulin-Dependent Protein Kinase IIα in Rat Spinal Cord and Midbrain During Induction of Morphine Analgesic Tolerance

Ahmadi

Rashidi

2016

Gene Cell Tissue

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IntroductionCalcium/calmodulin-dependent protein kinase II (CaMKII) which is highly expressed in the hippocampus is known to play a pivotal role in reward-related memories and morphine dependence.MethodsIn the present study, repeated morphine injections once daily for 7 days was done to induce morphine tolerance in male Wistar rats, after which gene expression profile of α-isoform of CaMKII (CaMKIIα) in the hippocampus was evaluated upon discontinuation of morphine injection over 21 days of morphine withdrawal. Control groups received saline for 7 consecutive days. For gene expression study, rats’ brains were removed and the hippocampus was dissected in separate groups on days 1, 3, 7, 14, and 21 since discontinuation of of morphine injection. A semi-quantitative RT-PCR method was used to evaluate the gene expression profile.ResultsTolerance to morphine was verified by a significant decrease in morphine analgesia in a hotplate test on day 8 (one day after the final repeated morphine injections). Results showed that gene expression of CaMKIIα at mRNA level on day 1, 3, 7, 14 and 21 of morphine withdrawal was significantly altered as compared to the saline control group. Post hoc Tukey's test revealed a significantly enhanced CaMKIIα gene expression on day 14.DiscussionIt can be concluded that CaMKIIα gene expression during repeated injections of morphine is increased and this increase continues up to 14 days of withdrawal then settles at a new set point. Therefore, the strong morphine reward-related memory in morphine abstinent animals may, at least partly be attributed to, the up-regulation of CaMKIIα in the hippocampus over 14 days of morphine withdrawal.

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Section: Introductionmentioning

confidence: 99%

Gene Expression Profile of Calcium/Calmodulin-Dependent Protein Kinase IIα in Rat Spinal Cord and Midbrain During Induction of Morphine Analgesic Tolerance

Ahmadi

Rashidi

2016

Gene Cell Tissue

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“…The stimulation of this path causes the appearance of the signal for need and demand of narcotic drug. The reward system section of the brain (mesolimbic) mainly uses dopamine as neurotransmitter to interfere the appearance of emotion and excitement [1][2][3]. Other effective divisions in addiction to narcotic drug are adrenergic and glutamatergic synapses [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

The Ultra Structure Effects of Morphine on Locous Coeruleus, in N-Mary Rat

Heidari¹,

Mahbouobipour²,

Heidari³

et al. 2014

nova-jmbs

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Addiction is a chronic disease in which addicted individual needs to consume narcotic drug in a compulsive uncontrollable manner and shows simultaneously an entire dependent behavior as following: looking for drug, consuming drug, recovering temporarily. We aim to study the correlation of LC nuclei and its possible ultra structural changes in the area of LC synaptic neurons, like pre-synaptic density (PSD) and synaptic connection zone (SCZ) with chronic consumption of morphine among the treatment, control and placebo groups in rats. In This descriptive and experimental study, 36 N -Mary male rats, were randomly divided into three groups, morphine (n=12), placebo (n=12) and control (n=12) groups. In the morphine group, complex of morphine (0.1, 0.2, 0.3 and 0.4) mg / ml and in the placebo (sucrose) group complex of sucrose (% 0.3) were used for 21 days. After the end of drug treatment the animals were scarified and perfuse intra cardinally and finally the LC samples were taken for ultra-structural studies. Several morphological and ultra structural changes in the different area of synapses of the treatment groups were observed after chronic consumption of morphine, like changes in dendrite plasticity of locus coeruleus nuclei, bend in the area of synaptic connection zone (SCZ), changes in bend of membrane connection of pre and post synaptic area and change in dendrite spines of Locus Coeruleus nuclei. It seems that there is a relation between ultra structural of synapses in locus coeruleus neurons and chronic consumption of morphine.

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Electroacupuncture attenuates ketamine-induced neuronal injury in the locus coeruleus of rats through modulation of the CAMK II/CREB pathway

Miao

et al. 2023

Brain Research Bulletin

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Examining the effect of the CaMKII inhibitor administration in the locus coeruleus on the naloxone-precipitated morphine withdrawal signs in rats

Cited by 6 publications

References 29 publications

Gene Expression Profile of Calcium/Calmodulin-Dependent Protein Kinase IIα in Rat Spinal Cord and Midbrain During Induction of Morphine Analgesic Tolerance

Gene Expression Profile of Calcium/Calmodulin-Dependent Protein Kinase IIα in Rat Spinal Cord and Midbrain During Induction of Morphine Analgesic Tolerance

The Ultra Structure Effects of Morphine on Locous Coeruleus, in N-Mary Rat

Electroacupuncture attenuates ketamine-induced neuronal injury in the locus coeruleus of rats through modulation of the CAMK II/CREB pathway

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