Examining for possible non-thermal effects during heating in a microwave oven

Shazman, Asher; Mizrahi, S.; Cogan, Uri; Shimoni, Eyal

doi:10.1016/j.foodchem.2006.08.024

Cited by 115 publications

(55 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cell viability experiments revealed that the MW treatment was not bactericidal, since 88% of the cells were recovered after radiation. It is proposed that one of the effects of exposing E. coli cells to MW radiation under sublethal temperature conditions is that the cell surface undergoes a modification that is electrokinetic in nature, resulting in a reversible MW-induced poration of the cell membrane.The effects of MW radiation on microorganisms have been studied and debated for more than half a century (3,4,10,12,17,20,28,29,35). The nature of the debate surrounding this interaction has often referred to the existence of so-called specific microwave (MW) effects that are nonthermal in nature (4,10,13,17,20,28,29).…”

mentioning

confidence: 99%

“…The nature of the debate surrounding this interaction has often referred to the existence of so-called specific microwave (MW) effects that are nonthermal in nature (4,10,13,17,20,28,29). Much has been published supporting the notion that a range of specific MW effects exist and can be identified in terms of their manifestations on cell physiology (2,4,10,13,27,28).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Specific Electromagnetic Effects of Microwave Radiation on Escherichia coli

Shamis

Taube

Mitik-Dineva

et al. 2011

Appl Environ Microbiol

View full text Add to dashboard Cite

The present study investigated the effects of microwave (MW) radiation applied under a sublethal temperature on Escherichia coli. The experiments were conducted at a frequency of 18 GHz and at a temperature below 40°C to avoid the thermal degradation of bacterial cells during exposure. The absorbed power was calculated to be 1,500 kW/m 3 , and the electric field was determined to be 300 V/m. Both values were theoretically confirmed using CST Microwave Studio 3D Electromagnetic Simulation Software. As a negative control, E. coli cells were also thermally heated to temperatures up to 40°C using Peltier plate heating. Scanning electron microscopy (SEM) analysis performed immediately after MW exposure revealed that the E. coli cells exhibited a cell morphology significantly different from that of the negative controls. This MW effect, however, appeared to be temporary, as following a further 10-min elapsed period, the cell morphology appeared to revert to a state that was identical to that of the untreated controls. Confocal laser scanning microscopy (CLSM) revealed that fluorescein isothiocyanate (FITC)-conjugated dextran (150 kDa) was taken up by the MW-treated cells, suggesting that pores had formed within the cell membrane. Cell viability experiments revealed that the MW treatment was not bactericidal, since 88% of the cells were recovered after radiation. It is proposed that one of the effects of exposing E. coli cells to MW radiation under sublethal temperature conditions is that the cell surface undergoes a modification that is electrokinetic in nature, resulting in a reversible MW-induced poration of the cell membrane.The effects of MW radiation on microorganisms have been studied and debated for more than half a century (3,4,10,12,17,20,28,29,35). The nature of the debate surrounding this interaction has often referred to the existence of so-called specific microwave (MW) effects that are nonthermal in nature (4,10,13,17,20,28,29). Much has been published supporting the notion that a range of specific MW effects exist and can be identified in terms of their manifestations on cell physiology (2,4,10,13,27,28). For example, Dreyfuss and Chipley examined the effects of MW radiation (2.45 GHz) at sublethal temperatures on the metabolic activities of a range of enzymes expressed by the bacterium Staphylococcus aureus (10). These results suggested that MW radiation affected S. aureus cells in a way that could not have been explained solely by thermaleffect theories. It has also been found that Burkholderia cepacia bacteria could be wholly inactivated using MW radiation at sublethal temperatures at a frequency of 20 GHz (2). Samarketu et al. (25) examined the effects of MW radiation at a frequency of 9.575 GHz on the physiological behavior of Cyanobacterium dolium (Anabaena dolium). The authors suggested that MW radiation nonthermally induced different biological effects by changing the protein structures by differentially partitioning the ions and altering the rates and/or directions of biochemical reactions (25)...

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

Specific Electromagnetic Effects of Microwave Radiation on Escherichia coli

Shamis

Taube

Mitik-Dineva

et al. 2011

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…5-8 in Table 7) revealed that the role of the MW non-thermal effect was not as obvious and recommended further investigation. In addition to the above views, other researchers believed that the MW non-thermal effect did not exist according to their research results [152,153].…”

Section: Discussion Of Mw Non-thermal Effectmentioning

confidence: 96%

Study and application status of microwave in organic wastewater treatment – A review

Wang

2016

Chemical Engineering Journal

142

View full text Add to dashboard Cite

“…Also, some of the hypotheses behind athermal effects of microwave heating suggest that microwave radiation may change the solvent properties of water. 20 Data on the influence of microwave heating on the isomerisation and racemisation of amino acids have been published in the recent years. Studies applied on whole and skim milk was conducted by Petrucelli and Fisher (1994).…”

Section: Discussionmentioning

confidence: 99%

Physical and Chemical Charateristics of Milk. Variation due to Microwave Radiation

Dumuţa¹,

Giurgiulescu²,

Mihaly‐Cozmuta³

et al. 2011

Croat. Chem. Acta

View full text Add to dashboard Cite

Abstract. The purpose of this paper was to see what effects microwaves have on the physical-chemical composition of the raw milk. The results showed that the averages of fat, protein, dry substance and lactose concentrations decreased during the microwave exposure while the density averages increased. This variation was characterized by the critical time moment of 31.71 s, corresponding to the moment when a milk sample exposed to the microwaves action undergoes the first more significant alteration of its physical-chemical composition. (doi: 10.5562/cca1785)

show abstract

Examining for possible non-thermal effects during heating in a microwave oven

Cited by 115 publications

References 11 publications

Specific Electromagnetic Effects of Microwave Radiation on Escherichia coli

Specific Electromagnetic Effects of Microwave Radiation on Escherichia coli

Study and application status of microwave in organic wastewater treatment – A review

Physical and Chemical Charateristics of Milk. Variation due to Microwave Radiation

Contact Info

Product

Resources

About