Examining a transparent object with a linnik tomographic microscope

Vishnyakov, G. N.; Zakaryan, K.; Левин, Г. Г.; Streletskaya, Elena A.

doi:10.1007/bf02504204

Cited by 7 publications

(3 citation statements)

References 4 publications

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“…For example, phase and confocal diffuse reflectance microscopy is, in general, a very promising tool. [34][35][36][37][38][39][40] However, this technique is not well suited for imaging lowscattering, low-refractive microlymphatics at the single-cell level, especially with scattering and refractive background noise from surrounding connective tissue. Powerful fluorescent imaging can only be applied to fluorescent samples, and most structures in rat mesentery in its native state are non-or weakly fluorescent.…”

Section: Introductionmentioning

confidence: 99%

In vivo integrated flow image cytometry and lymph/blood vessels dynamic microscopy

Galanzha

Tuchin²,

Zharov

2005

J. Biomed. Opt.

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The high spatial resolution (approximately 350 nm) transmission digital microscopy (TDM) was developed for real time in vivo imaging of microlymphatics of rat mesentery at a single cell level without any contrast agent. The main mesenteric microstructures (lymph-vessel diameter, valve geometry, cells, etc.) and their dynamics (wall motion, valve function, cell velocity, etc.) were monitored with TDM. Depending on structure size, different magnifications were used to image relatively large whole lymphangion (x4 to x10) as well as to image single cells (x40 to x100) in lymph and blood flow including estimation of their shape, size, and aggregation state. Various potential applications of the TDM for in vivo studies are discussed, including visualization of circulating cells in lymph and blood flows, studying the kinetics of platelets, leukocyte rolling, as well as imaging absorbing nonfluorescent mesentery structures and leukocytes with a high optical resolution.

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Section: Introductionmentioning

confidence: 99%

In vivo integrated flow image cytometry and lymph/blood vessels dynamic microscopy

Galanzha

Tuchin²,

Zharov

2005

J. Biomed. Opt.

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“…The erythrocyte size is increased during experiment. 5.S1O The lymphocytes were investigated with time spacing 120 seconds during 4-5hours (Fig.5). Amplitude oscillation of dry weight is 10%.…”

Section: Resultsmentioning

confidence: 99%

“…The defmition of the dry material weight is P= JJfp(x,y,z)dxdydz, (5) in which we substitute for p from (4) We note that (6) contains the cell volume V, which cannot be determined accurately from a single-phase projection and needs to be calculated by tomographic methods. However, if the object is spherical (as with human lymphocytes), one can estimate the volume from Veq = 4nReq3/3 in terms ofthe equivalent radius Req, which is determined as the radius of inertia in the image:…”

Section: Optical Setup and Technical Features Of The Computerized Intmentioning

confidence: 99%

Application of computerized interference microscope for monitoring oscillations of dry cell weight and morphology of living cells

et al. 2001

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Interference microscopy technique allows implementing the quantitative analysis of a living transparent cell, for example, to measure dry cell weight and cell density spatial distribution. The authors have created the microscope for quantitative analysis of living cells and observation of the dynamics of cell vital activity. Computer-aided processing of interferograms using phase shift technique, allows carrying out continuous (over 12 hours and more) monitoring of quantitative parameters of living cells with time interval between measurements less than one minute. RMS error for dry weight of non-living fixed test object using this microscope is about 1%. Numerous experiments on living lymphocytes and erythrocytes resulted in detecting the oscillations of dry weight. Spectral analysis of obtained time series has allowed allocating harmonics with the period from 2 hours to 1 5 minutes. Morphometric measurements of the same cells and cross correlation analysis of these data with oscillations of a dry mass were carried out also.

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The metabolic component of cellular refractivity and its importance for optical cytometry

Tychinsky

2009

Journal of Biophotonics

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Initially, it has been shown that the phase thickness and refractivity (the latter interpreted as the difference of the refractivity indices of an object and surrounding medium) depend on the functional state of mitochondria. The refractivity of various objects decreased in response to energy depletion. This dependence was then demonstrated for other biological objects such as cyanobacteria, chloroplasts and human cells. This general response brought about the hypothesis of a certain "universal" factor that links the variable (or metabolic) component of refractivity with the object's functional state. However, the origin of this phenomenon remains unknown. Our hypothesis is founded on the dependence of polarization of bound water molecules and the activity of metabolic processes. Here, we show the results of measurements of metabolic component of refractivity different bio-objects (mitochondria, chloroplasts, spores, cancer cells) obtained using the Coherent Phase Microscope "Airyscan". Estimations indicated high (up to n approximately = 1.41-1.45) values for the equivalent refractive index of structured water in cells.

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Examining a transparent object with a linnik tomographic microscope

Cited by 7 publications

References 4 publications

In vivo integrated flow image cytometry and lymph/blood vessels dynamic microscopy

In vivo integrated flow image cytometry and lymph/blood vessels dynamic microscopy

Application of computerized interference microscope for monitoring oscillations of dry cell weight and morphology of living cells

The metabolic component of cellular refractivity and its importance for optical cytometry

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