Examination of the envelope glycoprotein of yellow fever vaccine viruses with monoclonal antibodies

Barrett, Alan D.T.; Pryde, A.; Medlen, A. R.; Ledger, T. N.; Whitby, J. E.; Gibson, C.A.; Desilva, Mark G.; Groves, D.J.; Langley, David; Minor, Philip D.

doi:10.1016/0264-410x(89)90196-5

Cited by 33 publications

(15 citation statements)

References 6 publications

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“…The monolayers were then covered with 5 ml of 1% Noble agar (Sigma) in EMEM supplemented with 2% FCS. Viral plaques were visualized after 4 days by staining with neutral red (Barrett et al, 1989). TCIDso assays were made using HeLa cells.…”

Section: Cells and Virusmentioning

confidence: 99%

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Attenuation of virulence of flaviviruses following passage in HeLa cells

Dunster

Gibson

Stephenson

et al. 1990

Journal of General Virology

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Section: Cells and Virusmentioning

confidence: 99%

“…Both parent and HeLa passage 6 (p6) viruses were tested in plaque reduction neutralization tests, which were carried out on LLC-MK2 monolayers, as described in Barrett et al (1989).…”

Section: Cells and Virusmentioning

confidence: 99%

Attenuation of virulence of flaviviruses following passage in HeLa cells

Dunster

Gibson

Stephenson

et al. 1990

Journal of General Virology

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“…A plaque assay procedure was used to determine the virus infectivity titre (Barrett et al, 1989); LLC-MK2 cells were used for all assays.…”

Section: Infectivity Titrationmentioning

confidence: 99%

In vitro homotypic and heterotypic interference by defective interfering particles of West Nile virus

Debnath

Tiernery

Sil

et al. 1991

Journal of General Virology

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Defective interfering (DI) particles of the flavivirus West Nile (WN) were generated after as few as two high multiplicity serial passages in Vero and LLC-MK2 cells. Six cell lines (Vero, LLC-MK2, L929, HeLa, BHK-21 and SW13) were used to assay interference by DI particles in a yield reduction assay. Interference was found to vary depending on the cell type used. The highest levels of interference were obtained in LLC-MK2 cells, whereas no detectable effect was observed in BHK-21 and SW13 cells. The ability of DI virus to be propagated varied depending on the cell line used; no detectable propagation of DI virus was observed in SW 13 cells. Optimum interference was obtained following co-infection of cells with DI virus and standard virus at a multiplicity of 5. Interference between DI and standard viruses occurred only when they were co-infected or when cells were infected with DI virus 1 h before standard virus. Investigation of heterotypic interference by DI particles of WN virus strains from Sarawak, India and Egypt revealed that interference was dependent on the strain of WN virus or flavivirus used as standard virus. A measure of the similarity between five strains of WN virus and other flaviviruses was made on the basis of interference by DI viruses, and was found to be similar to that based on haemagglutination inhibition tests using a panel of monoclonal antibodies.

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“…MAb B39 represents one of a panel of E protein-reactive MAbs raised against the 17DD substrain vaccine virus manufactured in Brazil (17DD-Braz), characterized previously as a YF type-specific MAb (Ledger et al, 1992). In plaque reduction neutralization tests (PRNTs), (Barrett et al, 1989).…”

mentioning

confidence: 99%

“…performed in monolayers of SW13 cells as described by Barrett et al (1989), MAb B39 neutralized the World Health Organization secondary seed lot of the 17D-204 substrain vaccine virus (17D-204-WHO) to high titre (4 log "! units), but failed to neutralize any of the other YF virus strains tested, including 17DD-Braz (Barrett et al, 1989).…”

mentioning

confidence: 99%