2002
DOI: 10.1002/j.1939-4640.2002.tb02306.x
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Examination of the Binding Ability of Bovine Spermatozoa to the Zona Pellucida as an Indicator of Fertility

ANDREA G. BRAUNDMEIER,
JILL M. DEMERS,
ROGER D. SHANKS
et al.

Abstract: Despite the development of many new techniques, laboratory assays still do not predict male fertility accurately. To identify targets for laboratory assessment, we first need to determine which steps in fertilization are most often defective in subfertile males. We developed a competitive in vitro fertilization assay in which spermatozoa from 2 different males, stained with different lipophilic dyes, are incubated together with oocytes in a droplet. By exposing mixed spermatozoa to the same oocytes, this assay… Show more

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Cited by 22 publications
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