Examination of Interchromosomal Interactions in Vegetatively Growing Diploid<i>Schizosaccharomyces pombe</i>Cells by Cre/loxP Site-Specific Recombination

Molnár, Mónika; Kleckner, Nancy

doi:10.1534/genetics.107.082826

Cited by 27 publications

(27 citation statements)

References 45 publications

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“…For this reason, we devised a set of newly generated yeast strains with unbiased CRs departing from a single genetic background. We produced two pericentric inversions and eight reciprocal translocations encompassing the three chromosomes using a Cre-loxP system developed by Molnar and Kleckner 35 ( Fig. 2a; Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Genome architecture is a selectable trait that can be maintained by antagonistic pleiotropy

et al. 2013

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Chromosomal rearrangements are mutations contributing to both within and between species variation; however their contribution to fitness is yet to be measured. Here we show that chromosomal rearrangements are pervasive in natural isolates of Schizosaccharomyces pombe and contribute to reproductive isolation. To determine the fitness effects of chromosome structure, we constructed two inversions and eight translocations without changing the coding sequence. We show that chromosomal rearrangements contribute to both reproductive success in meiosis and growth rate in mitosis with a strong genotype by environment interaction. These changes are accompanied by alterations in gene expression. Strikingly, we find several examples leading to antagonistic pleiotropy. Even though chromosomal rearrangements may have a deleterious effect during sexual reproduction, some compensate with a strong growth advantage in mitosis. Our results constitute the first quantification of fitness effects caused by de novo mutations that result in chromosomal rearrangement variation and suggest a mechanism for their maintenance in natural populations.

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Section: Resultsmentioning

confidence: 99%

Genome architecture is a selectable trait that can be maintained by antagonistic pleiotropy

et al. 2013

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“…The territorial organization of interphase chromosomes is now generally accepted as a basic principle of nuclear organization in both animals and plants (Shaw et al 2002;Pecinka et al 2004;Berr et al 2006), and may even hold for single-cell eukaryotes, such as budding and fission yeast (Bystricky et al 2005;Molnar and Kleckner 2008). However, severe limitations in our present knowledge of the functional architecture of CTs and the nucleus at large become obvious when we consider currently proposed models (Fig.…”

Section: Models Of Nuclear Architecture: Open Questions and Experimenmentioning

confidence: 99%

Chromosome Territories

Cremer¹,

Cremer²

2010

Cold Spring Harbor Perspectives in Biology

955

813

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Chromosome territories (CTs) constitute a major feature of nuclear architecture. In a brief statement, the possible contribution of nuclear architecture studies to the field of epigenomics is considered, followed by a historical account of the CT concept and the final compelling experimental evidence of a territorial organization of chromosomes in all eukaryotes studied to date. Present knowledge of nonrandom CT arrangements, of the internal CT architecture, and of structural interactions with other CTs is provided as well as the dynamics of CT arrangements during cell cycle and postmitotic terminal differentiation. The article concludes with a discussion of open questions and new experimental strategies to answer them.

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“…In addition to their distinctive chromatin structures, these regions display distinctive subnuclear localizations. The fission yeast nucleus is structured (17)(18)(19)(20)(21)(22)(23), and the nuclear proteome is not uniformally distributed (24). One pole of the interphase nucleus is defined by the microtubule organizing center or spindle-pole body (SPB) spanning the nuclear envelope.…”

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Subnuclear relocalization and silencing of a chromosomal region by an ectopic ribosomal DNA repeat

Jakočiūnas¹,

Jordö²,

Mebarek³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Our research addresses the relationship between subnuclear localization and gene expression in fission yeast. We observed the relocalization of a heterochromatic region, the mating-type region, from its natural location at the spindle-pole body to the immediate vicinity of the nucleolus. Relocalization occurred in response to a DNA rearrangement replacing a boundary element (IR-R) with a ribosomal DNA repeat (rDNA-R). Gene expression was strongly silenced in the relocalized mating-type region through mechanisms that differ from those operating in wild type. Also different from the wild-type situation, programmed recombination events failed to take place in the rDNA-R mutant. Increased silencing and perinucleolar localization depended on Reb1, a DNAbinding protein with cognate sites in the rDNA. Reb1 was recently shown to mediate long-range interchromosomal interactions in the nucleus through dimerization, providing a mechanism for the observed relocalization. Replacing the full rDNA repeat with Reb1-binding sites, and using mutants lacking the histone H3K9 methyltransferase Clr4, indicated that the relocalized region was silenced redundantly by heterochromatin and another mechanism, plausibly antisense transcription, achieving a high degree of repression in the rDNA-R strain.nuclear organization | gene silencing | chromatin | boundary elements | antisense transcription E ukaryotic genomes are spatially organized within the nucleus (reviewed in refs. 1-5). In interphase, chromatin domains and entire chromosomes tend to occupy defined positions relative to each other or to nuclear landmarks. Chromosomal regions capable of associating with the nuclear periphery, with nuclear substructures, or with each other have been proposed to function as anchors underlying this organization (4-7). Coupled with a nonuniform distribution of proteins in the nucleus, the dynamic, directed localization of chromosomal regions to subnuclear compartments might influence and perhaps regulate gene expression and recombination. Both stable associations and transient contacts might impose lasting marks on the regions that participate in them (8, 9). Despite our increasingly detailed view of subnuclear organization, however, the two fundamental questions of how the organization is achieved and of its physiological significance remain largely open.In the fission yeast Schizosaccharomyces pombe, extended silent heterochromatic domains are found at the three centromeres, at the telomeres of chromosome 1 and 2, and in the right arm of chromosome 2, in the mating-type region (10-16). Large clusters of rDNA repeats at both ends of chromosome 3 are also partially heterochromatic (13,16). In addition to their distinctive chromatin structures, these regions display distinctive subnuclear localizations. The fission yeast nucleus is structured (17)(18)(19)(20)(21)(22)(23), and the nuclear proteome is not uniformally distributed (24). One pole of the interphase nucleus is defined by the microtubule organizing center or spindle-pole body (SPB) spanning ...

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Examination of Interchromosomal Interactions in Vegetatively Growing DiploidSchizosaccharomyces pombeCells by Cre/loxP Site-Specific Recombination

Cited by 27 publications

References 45 publications

Genome architecture is a selectable trait that can be maintained by antagonistic pleiotropy

Genome architecture is a selectable trait that can be maintained by antagonistic pleiotropy

Chromosome Territories

Subnuclear relocalization and silencing of a chromosomal region by an ectopic ribosomal DNA repeat

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