Examination of Growth Hormone (<I>GH</I>) Gene Polymorphism and its Association with Body Weight and Selected Body Dimensions in Ducks

Abstract

MAZUROWSKI A., FRIESKE A., KOKOSZYÑSKI D., MROCZKOWSKI S., BERNACKI Z., WILKANOWSKA A. 2015. Examination of growth hormone (GH) gene polymorphism and its association with body weight and selected body dimensions in ducks. Folia Biologica (Kraków) 63: 43-50.The main objective of the study was to assess the polymorphism in intron 2 of the GH gene and its association with some morphological traits (body weight-BW, length of trunk with neck-LTN, length of trunk-LT, chest girth-CG, length of breast bone-LBB, length of shank-LS). Polymorphism in intron 2 of the GH gene was evaluated for four duck populations (Pekin ducks AF51, Muscovy ducks from a CK and CRAMMLCFF mother and Mulard ducks). Genetic polymorphism was determined with the PCR-RFLP method using the BsmFI restriction enzyme. In the studied duck sample two alleles (GH + and GH 6 ) and three genotypes (GH/TT, GH/CT, GH/CC) were found at locus GH/BsmFI. In both groups of Muscovies and in Mulards the dominant allele was GH 6 . On the contrary in Pekin ducks AF51, the frequency of both alleles was found to be similar. The most frequent genotype in the examined ducks was GH/TT. In Pekin ducks AF51 three genotypes were observed, while in Mulard ducks and in male Muscovy ducks from a mother marked as CK, two genotypes (GH/TT and GH/CT) were identified. Muscovy duck females from a CK mother and all males and females of Muscovy duck from a CRAMMLCFF mother were monomorphic with only the GH/TT genotype detected. The results showed that males of Pekin duck AF51 with the GH/TT genotype were characterized by higher (P<0.01) BW value than those with the GH/CC and GH/CT genotype. In females of Pekin ducks AF51, this same trend was observed; individuals with GH/TT genotype were superior (P<0.05 and P<0.01) to birds with two other detected genotypes in respect to BW, CG, LBB and LS. In the case of Mulards, ducks with the GH/TT genotype were distinguished by higher values of all evaluated traits compared to ducks with GH/CT and GH/CC genotypes, however most of the recorded differences were not significant. The only trait markedly impacted (P<0.05) by the polymorphism of the GH gene intron 2 was the LS value in males.

Revisit the Development of Native Indonesian Chickens from Red Jungle Fowls (Gallus gallus bankiva) to Commercial Chickens

“…Dengan demikian gen cGH dapat digunakan sebagai kandidat gen pertumbuhan pada ayam broiler Thailand. Keragaman Growth Hormone Gene pada intron 2 dalam hubungannya dengan beberapa sifat morfologi (berat badan, panjang punggung sampai leher, panjang tubuh, lingkar dada, panjang tulang dada dan panjang shank) telah dilaporkan pada itik Pekin, entok, dan itik liar dan didapatkan bahwa gen GH pada intron 2 bersifat polimorfik pada itik Pekin strain AF 51, namun monomorfik pada ternak entok, sehingga pada itik Pekin genotipe GH/TT dapat dipergunakan sebagai marker bobot badan (Mazurowski et al 2015). Polimorfisme gen GH ditemukan pada lokus g.2248G dan gen GHR pada g.565G> A GHR pada ayam Kampung, broiler Cobb, F1, dan F2 hasil silang antara ayam Kampung dengan ayam broiler menggunakan PCR-RFLP dan enzim restriksi EcoRV dan Eco72I.…”

Evaluation of Growth Performance, Blood Metabolites and Gene Expression Analysis in Egyptian Sheep Breeds, in Relation to Age

“…It has been mapped to chromosome 11 in Ovis aries.Growth hormone (GH) gene is a promising candidate gene for farm animals' genetic selection. It has many physiological functions, such as promoting muscle and bone growth, enhancing milk production for females and also semen quality for males and development of mammary gland, regulation of fat content, it plays an important role in postnatal longitudinal growth and development, tissue growth, lactation, reproduction, as well as protein, lipid, and carbohydrate metabolism in sheep (Kumariet al,2013;Jia et al, 2014;Mazurowski et al, 2015;Farag et al, 2016;Abdelmoneim et al, 2017).…”

Genetic Resource of Muscovy Duck (Cairina moschata): Profile and Potential Production as Meat Producer

“…Melalui MAS telah teridentifikasi gen atau marker yang terkait dengan sifat pertumbuhan, karkas, daging dan sifat reproduksi ternak itik Taisya (Chang et al 2012), babi (Dvořáková et al 2012), ayam (Bhattacharya & Chatterjee 2013) dan sapi (Huang et al 2013). Keragaman growth hormone gene (gen GH) pada intron 2 dalam hubungannnya dengan beberapa sifat morfologi (berat badan, panjang punggung sampai leher, panjang tubuh, lingkar dada, panjang tulang dada dan panjang shank) telah dilaporkan pada itik Pekin, entok dan itik liar dan didapatkan bahwa gen GH pada intron 2 bersifat polimorfik pada itik Pekin strain AF 51, namun monomorfik pada ternak entok, sehingga pada itik Pekin genotipe GH/TT dapat dipergunakan sebagai marker bobot badan (Mazurowski et al 2015). Penelitian pada lima single nucleotide polymorphisms (SNP) 5′-flanking regions growth hormone (GH), prolactin (PRL) dan pituitary-specific transcription factor (Pit-1) genes, menyimpulkan bahwa terdapat empat SNP yang dapat dipergunakan sebagai marker untuk meningkatkan produksi telur entok (Zhang et al 2015).…”