Examination of Brevibacterium linens by an Electrophoretic Zymogram Technique

Foissy, H.

doi:10.1099/00221287-80-1-197

Cited by 45 publications

(12 citation statements)

References 21 publications

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“…IEF and lipase zymography were carried out on some of the CLP; most of those in which activity was detected showed two to four active bands. Zymography has been applied to detect a wide variety of enzyme activities (24,25). Tributyrin and butteroil have been successfully employed in zymography to detect lipase activities (26).…”

Section: Resultsmentioning

confidence: 99%

Composition and activity of commercial triacylglycerol acylhydrolase preparations

Bjurlin¹,

Bloomer²,

Haas

2001

J Americ Oil Chem Soc

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Commercial lipase preparations were surveyed to determine gross composition, amounts of nonprotein impurities, and esterolytic and lipolytic activities. Most of the 34 commercial lipase preparations contained more than 80% nonproteinaceous material, with salt and carbohydrate being the most abundant materials. The tributyrin hydrolase activity of these commercial lipase preparations was determined and expressed as lipase/esterase forestomach units (LFU). Tributyrin hydrolase activity ranged from negligible (5.3 LFU/g) to very high (>1,000,000 LFU/g). Aspergillus and Penicillium preparations were low in tributyrin hydrolase activity. Candida rugosa preparations were intermediate in activity. Preparations of porcine pancreas, Rhizomucor, Pseudomonas, and Rhizopus lipases exhibited a broad range of levels of activity. No relation between protein content and tributyrin hydrolase activity was observed. Isoelectric focusing of the proteins present in the preparations demonstrated the presence of between 2 and 27 isophoretically discrete bands in the isoelectric range of 3 to 9. Although there were many similarities of distribution of protein isoelectric points within genera and species, the preparations generally displayed unique patterns of isophoretically discrete protein bands. Lipase zymography demonstrated the presence of 0 to 7 isophoretically discrete lipase activities in each preparation, spanning the entire range of isoelectric points surveyed.Paper no. J9736 in JAOCS 78, 153-160 (February 2001).

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Section: Resultsmentioning

confidence: 99%

Composition and activity of commercial triacylglycerol acylhydrolase preparations

Bjurlin¹,

Bloomer²,

Haas

2001

J Americ Oil Chem Soc

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“…Several species of the genera Corynebacterium and Brevibacterium were isolated from a variety of habitats of elevated external osmolarities, like cheese, human skin or marine environments [20][21][22]. Therefore, they must have developed mechanisms to cope with moderate osmotic activities.…”

Section: Discussionmentioning

confidence: 99%

Compatible solutes in representatives of the genera Brevibacterium and Corynebacterium: Occurrence of tetrahydropyrimidines and glutamine

Frings¹

1993

FEMS Microbiology Letters

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The aim of our investigation was to study the haloadaptation of a number of species of Gram‐positive bacteria belonging to the genera Brevibacterium and Corynebacterium. We used two different HPLC‐techniques and 13C‐NMR spectroscopy for the identification of osmolytes (compatible solutes). The tetrahydropyrimidines (‘ectoines’) are the main compatible solutes in the genus Brevibacterium, whereas accumulation of glycine‐betaine and accumulation of synthesis of glutamine is mainly responsible for osmoadaptation in the genus Corynebacterium. Pipecolic acid, formerly described as a potential osmolyte synthesized de novo in C. ammoniagenes, does not contribute markedly to the solute pool, unless supplemented to the medium.

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“…R312 as has frequently been observed in many other species and Brevibacterium linens strains. 24) Specific esterase activities of the two strains studied were maximum during the stationary growth phase. A similar observation was reported for the esterase pNPA activity of Lactobacillus helveticus CNRZ 32.26) Comparison of specific esterase activities of Brevibacterium sp.…”

Section: Discussionmentioning

confidence: 90%