Examination of Bioluminescent Excitation Responses Using Empirical Orthogonal Function Analysis

Davis, James W.; Thosteson, Eric D.; Frey, Lee; Widder, Edith A.

doi:10.1109/oceans.2005.1639863

Cited by 8 publications

(4 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Introduction of a voltage offset to the photodiode measurements and substitution of larger area photodiodes are modifications that were recently made, and the latest version of the bathyphotometer was tested in the laboratory using cultures of the dinoflagellates Lingulodinium polyedrum and Pyrocystis fusiformis. As hoped, the excitation response curves produced, one of which is shown in Figure 6, were similar in form to those produced by the HIDEX 3 (Davis et al, 2005). This is a highly significant accomplishment as it provides proof-of-concept that a solid-state detector system can replicate the HIDEX III capability of identification of bioluminescent populations by excitation response patterns.…”

Section: Resultssupporting

confidence: 54%

Monitoring Bloom Dynamics of a Common Coastal Bioluminescent Ctenophore

Widder¹

2010

View full text Add to dashboard Cite

The long-term objective is to develop predictive models of bioluminescence potential in the coastal zone environment. OBJECTIVES In the coastal zone, watershed runoff and discharge of submarine groundwater can profoundly impact growth conditions of bioluminescent plankton on very short space and time scales. Bioluminescent blooms include dinoflagellate red tides, which are occurring more frequently, lasting longer and extending further off shore due to excessive nutrient loading from land-based runoff. Bioluminescent blooms of the carnivorous ctenophore Mnemiopsis leidyi that may be either seasonal or event driven can also develop on remarkably short time scales (Kremer, 1994) and also appear to be on the increase (Sullivan et al., 2001). The Ocean Research & Conservation Association's (ORCA) objective is to improve understanding of the conditions leading to bioluminescent blooms in coastal waters such that forecasting is possible. Achieving this requires more frequent, automated sampling of greater spatial density, a reduction in dependence upon hand sampling, and automating all aspects of the data collection and analysis processes. APPROACH Increasing the frequency of field sampling and removing the bias introduced by fair-weather sampling requires automation. Achieving greater spatial density of measurements in a sustainable manner requires reducing the cost of sensor systems through component-level integration. New technologies, such as ORCA's bathyphotometer to measure bioluminescence, are necessary to automate biological sample collection and processing. When hand sampling is still necessary, the processing should make use of automated methods for processing. ORCA's approach to monitoring bloom dynamics is to supplement the ORCA Kilroy network with low-cost, autonomous bathyphotometers, using the intensity of bioluminescence as an estimator of abundance and the flash kinetics to assist in distinguishing organisms.

show abstract

Section: Resultssupporting

confidence: 54%

Monitoring Bloom Dynamics of a Common Coastal Bioluminescent Ctenophore

Widder¹

2010

View full text Add to dashboard Cite

show abstract

“…The previously mentioned work of Cronin et al (2016) andMessiéet al (2019) demonstrate this. Additionally, Davis et al (2005) used an empirical orthogonal function analysis to not only identify dinoflagellate species, but also isolate individual flashes from mixed data sets acquired with the HIDEX. However, using biologically emitted light as an identification proxy for ecosystem monitoring only becomes useful when the majority of the community has had its kinetics described.…”

Section: Organism Emissionsmentioning

confidence: 99%

A review of mechanically stimulated bioluminescence of marine plankton and its applications

Letendre,

Twardowski,

Blackburn

et al. 2024

Front. Mar. Sci.

View full text Add to dashboard Cite

Bioluminescence is ubiquitous in marine ecosystems and found in uni- and multicellular organisms. Bioluminescent displays can be used to deter predators, attract mates, and lure and hunt prey. Mechanically stimulated flash kinetics of zooplankton and dinoflagellates are life stage-dependent and species-specific, and could prove effective at identification and monitoring biodiversity in bioluminescent species. Here, we provide a comprehensive review of mechanically stimulated bioluminescence for the main dinoflagellate and zooplankton clades in marine environments and assemble known flash kinetics and spectral emission data. Instruments and methods used in measuring bioluminescence are also discussed. Applications, research gaps, perspectives, and biases in approaches to studying bioluminescence are identified. Moreover, emission kinetics of most zooplankton are very poorly known and constitute a critical gap. Lastly, available knowledge is interpreted in terms of potential future changes in global bioluminescence driven by climate change.

show abstract

“…With its digital-signal-processor centered design, the ORCA BP is capable of implementing bioluminescence flash kinetics classification capabilities previously demonstrated with the spatial plankton analysis technique (SPLAT) system and HIDEX BP [5,6].…”

Section: Orca Bathyphotometermentioning

confidence: 99%

New technology for Ecosystem-Based Management: Marine monitoring with the ORCA Kilroy Network

et al. 2009

View full text Add to dashboard Cite

Ecosystem-Based Management (EBM) requires marine monitoring in real time with high temporal frequency and high spatial density. It also requires sensors that can provide direct measurements of biological processes and the means to track water movement and evaluate water quality. These requirements can not realistically be met using huge amalgamations of the independent instruments traditionally used for marine monitoring. To do so would result in marine observatories that are prohibitively expensive, unnecessarily obtrusive, inefficient in power use, difficult to deploy and maintain, with sensors inappropriate for extended deployment and with an excessive level of redundant components. The Ocean Research & Conservation Association (ORCA), in an effort to better meet the need for marine monitoring, has designed and is testing the ORCA Kilroy Network, an observatory designed as a whole system down to the sensors at the component level. The ORCA Network consists of a wireless network of remote semiautonomous marine sensor systems created by ORCA, and a central supervisory system that directs operations of the remote systems, collects data, and relays that via the Internet through a standard SOAP web service interface to a geospatial database. The network design is based on that of industrial control systems, where a central computer coordinates remote and mostly autonomous systems using Modbus, a mature, open-standard, fieldbus protocol. In this case, the coordination and data transfer are over GSM cellular Internet connections on a wide scale and cabled RS-485 connections at the station scale.To date, four remote subsystems of the ORCA Kilroy network are in use. Each is designed to take advantage of a shared power and communications infrastructure, and is integrated at the component level to lower cost, reduce size, and improve efficiency. Mass produced off-the-shelf parts have been selected over custom machined parts where possible. Power for an entire sensor station is shared, and to date, has been provided by a solar charged battery. First, the wireless and cabled communications are bridged by a device dubbed the ORCA Kilroy Voice (KV), which includes a GPS for position and UTC synchronization and acts as a GPRS Internet gateway to a sensor string -a shared serial communications and power line on which smart sensor systems can be daisy chained. Second, ORCA developed for use on that string a battery-operated sensor suite called ORCA Kilroy that from basic measurements, determines flow speed, flow direction, speed of sound, package orientation, turbidity, conductivity, temperature, water level, wave height, and wave period. Measurements are sent out upon request from the central supervisor through Modbus and are logged locally to nonvolatile memory to ensure no data is lost in the event of a power or communication outage. Third is the ORCA Kilroy I/O (KIO) -an adaptor providing serial access to the ORCA Network for instruments from other manufacturers. A forth system, also designed for sensor string operation, is...

show abstract

Examination of Bioluminescent Excitation Responses Using Empirical Orthogonal Function Analysis

Cited by 8 publications

References 7 publications

Monitoring Bloom Dynamics of a Common Coastal Bioluminescent Ctenophore

Monitoring Bloom Dynamics of a Common Coastal Bioluminescent Ctenophore

A review of mechanically stimulated bioluminescence of marine plankton and its applications

New technology for Ecosystem-Based Management: Marine monitoring with the ORCA Kilroy Network

Contact Info

Product

Resources

About