Examination of anonymous canine faecal samples provides data on endoparasite prevalence rates in dogs for comparative studies

Hinney, Barbara; Gottwald, Michaela; Moser, Jasmine; Reicher, Bianca; Schäfer, Bhavapriya Jasmin; Schaper, Roland; Joachim, Anja; Künzel, Frank

doi:10.1016/j.vetpar.2017.08.016

Cited by 14 publications

(10 citation statements)

References 64 publications

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“…Assemblages A and B are considered zoonotic [ 7 ]. The prevalence of G. intestinalis in dogs ranges from 4 to 36.5% in Europe [ 4 , 8 , 9 ]. The prevalence is higher in young animals (between 9 and 14 weeks of age, 62.4%) compared to puppies between 5 and 8 weeks of age (30.4–32.6%) [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Age and Giardia intestinalis Infection Impact Canine Gut Microbiota

et al. 2021

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Giardia intestinalis is a flagellated protozoan responsible for giardiosis (also called giardiasis in humans), the most prevalent and widespread parasitic infection in humans and mammals worldwide. The intestinal microbiota is highly diverse and any alteration in its composition may impact on the health of the host. While studies on the mouse model of giardiosis described the role of the gut microbiota in host susceptibility to infection by the parasite, little is known about the gut microbiota during natural infections in dogs and particularly in puppies. In this study, we monitored naturally G. intestinalis-infected puppies for 3 months and quantified cyst excretion every 2 weeks. All puppies remained subclinically infected during the sampling period as confirmed by fecal examination. In parallel, we performed 16S Illumina sequencing of fecal samples from the different time points to assess the impact of G. intestinalis infection on gut microbiota development of the puppies, as well as gut health markers of immunity such as fecal IgA and calprotectin. Sequencing results revealed that the canine fecal microbiota of Giardia-infected puppies becomes more complex and less diverse with increasing age. In addition, significant differences in the structure of the microbiota were observed between puppies with high and low Giardia cyst excretion. Chronic subclinical G. intestinalis infection appears to be associated with some detrimental structural changes in the gut microbiota. G. intestinalis-associated dysbiosis is characterized by an enrichment of facultative anaerobic, mucus-degrading, pro-inflammatory species and opportunistic pathogens, as well as a reduction of Lactobacillus johnsonii at specific time points. Calprotectin levels increased with age, suggesting the establishment of chronic low-grade inflammation in puppies. Further work is needed to demonstrate whether these alterations in the canine gut microbiota could lead to a dysbiosis-related disease, such as irritable bowel syndrome (IBS) or inflammatory bowel disease (IBD).

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Section: Introductionmentioning

confidence: 99%

Age and Giardia intestinalis Infection Impact Canine Gut Microbiota

et al. 2021

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“…Se encontró una prevalencia de endoparásitos del 37,2 % en la población de perros domiciliados de la zona metropolitana de Toluca, cifra mayor a la reportada en otros estudios nacionales e internacionales de perros con atención médica: en Ciudad de México, las prevalencias registradas han sido de 20 % y 21,3 % 9 , 29 , en Villahermosa, Tabasco, de 26,5 % 8 , en sitios en Estados Unidos, de 12,5 % 5 , en España, de 25 % 30 , y en Austria, de 6 % 6 .…”

Section: Discussionunclassified

“…En diversos estudios se han reportado prevalencias variables de endoparásitos en perros domiciliados: en Estados Unidos, se encontró una prevalencia nacional del 12,5 % 5 , en Austria, se reporta un 6 % 6 , en tanto que en Brasil, una mayor, de 43 % 7 . En México, las prevalencias son variables.…”

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Factores asociados con la presencia de endoparásitos y ectoparásitos en perros domiciliados de la zona metropolitana de Toluca, México

et al. 2021

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Introducción. Los endoparásitos y ectoparásitos en perros son de distribución mundial. La estrecha relación entre los perros y el hombre implica un riesgo de transmisión de parasitosis zoonóticas, por lo cual es necesario conocer las especies que parasitan a los perros de esta zona y determinar los factores asociados.Objetivos. Estimar la prevalencia de endoparásitos y ectoparásitos, identificarlos en perros domiciliados de la zona metropolitana de Toluca, México, y determinar la prevalencia de Dipyilidium caninum en pulgas del género Ctenocephalides spp.Materiales y métodos. Se recolectaron muestras de 402 perros que fueron llevados a consulta en cuatro hospitales de referencia de Toluca. En el diagnóstico de endoparásitos, se utilizaron las técnicas coproparasitoscópicas de frotis directo, flotación y sedimentación; además, se recolectaron ectoparásitos para su identificación taxonómica. Por último, la detección de D. caninum en pulgas se hizo mediante la reacción en cadena de la polimerasa (PCR).Resultados. El 37,2 % de los perros resultó positivo para endoparásitos. Los géneros o especies identificados fueron Toxocara spp., Giardia spp., Ancylostoma spp., Cystoisospora spp., D. caninum, Taenia spp. y Trichuris vulpis. Se determinó una prevalencia de ectoparásitos de 13,13 %. Se identificaron pulgas de las especies Ctenocephalides felis y C. canis, en tanto que solo un animal presentó parasitosis por Rhipicephalus sanguineus y otro por Trichodectes canis. La prevalencia de D. caninum en pulgas fue del 9,5 %.Conclusión. La prevalencia de endoparásitos fue de 37,2 % y, la de ectoparásitos, de 13,1 %. Por primera vez en México se hizo un análisis de endoparásitos y ectoparásitos en una misma población de perros, así como el diagnóstico molecular de D. caninum.

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“…canis in dogs should be carefully analysed because it has been shown to be dependent of the age of pets, a variable considered in some previous studies (Overgaauw & Nederland, 1997; Rubel et al ., 2003; Nijsse et al ., 2015) but not in that by Martin & Demonte (2008). Puppies less than one year old are more infected and, at the same time, they remain most of the time in the interior of homes and are usually underrepresented in studies that analyse faeces from public spaces (Hinney et al ., 2017). This is a possible reason why the presence of T .…”

Section: Discussionmentioning

confidence: 99%

Human density, economic level and frequency of canine helminths in Buenos Aires

Rubel

Flaibani

2021

J. Helminthol.

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The aim of this study was to explore through cross-sectional study the variation in the prevalence of parasitic helminths in canine faeces collected from green spaces of Buenos Aires according to the human density (HD) and economic level (EL) in the surroundings. HD and EL were considered as independent variables with three categories each. Twenty public squares (one hectare of surface) were randomly selected for each existing combination of the two independent variables. Ten random samples of fresh canine faeces were obtained in each square and analysed for helminths by the sedimentation and flotation techniques. The prevalence for each of the species was analysed using generalized linear models (GLM). The prevalence was modelled with a binomial error distribution and a logit link function. Helminth eggs were detected in 45 out of the 200 (22.5%) faecal samples collected and in 18 of the 20 green spaces sampled. The species observed were Ancylostoma caninum (13% of samples), Trichuris vulpis (8%) and Toxocara canis (4.5%). The GLM indicated that the prevalence of A. caninum in the slum areas (very high HD and very low EL) was higher than that in the other areas studied. However, the HD seemed to contribute more than the EL to the variations in the prevalence of A. caninum in faecal samples. The GLM showed no differences in the prevalence of the other parasite species for the different levels of the independent variables.

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Examination of anonymous canine faecal samples provides data on endoparasite prevalence rates in dogs for comparative studies

Cited by 14 publications

References 64 publications

Age and Giardia intestinalis Infection Impact Canine Gut Microbiota

Age and Giardia intestinalis Infection Impact Canine Gut Microbiota

Factores asociados con la presencia de endoparásitos y ectoparásitos en perros domiciliados de la zona metropolitana de Toluca, México

Human density, economic level and frequency of canine helminths in Buenos Aires

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