Exacerbation of symptomatic arthritis by cigarette smoke in experimental arthritis

Abstract

Introduction Epidemiologically, cigarette smoking is a well-known risk factor for the pathogenesis of rheumatoid arthritis (RA). However, there has been few plausible explanations why cigarette smoking aggravated RA. We investigated the causal effect of smoking in experimental model of arthritis development. Methods During induction of experimental arthritis with collagen challenge, mice were exposed to a smoking environment with 3R4F cigarettes. Generated smoke was delivered to mice through a nose-only exposure chamber (ISO standard 3308). Human cartilage pellet was challenged by cigarette smoke extract to identify citrullinating potential in vitro. Results Cigarette smoke exacerbated arthritis in a collagen-induced arthritis (CIA) model. Exposure to smoke accelerated the onset of arthritis by 2 weeks compared to the conventional model without smoke. Citrullination of lung tissue as well as tarsal joints were revealed in smokeaggravated CIA mice. Interestingly, tracheal cartilage was a core organ regarding intensity and area size of citrullination. The trachea might be an interesting organ in viewpoint of sharing cartilage with joint and direct smoke exposure. Anti-CCP antibodies were barely detected in the serum of CIA mice, they were significantly elevated in cigarette smoke group. Citrullinated antigens were increased in the serum of smoke-exposed mice. Lastly, a cigarette smoke extract enhanced human cartilage citrullination in vitro. Conclusions Missing link of arthritic mechanism between smoke and RA could be partially explained by tracheal citrullination. To control tracheal cartilage citrullination may be beneficial for

The Effect of Inhaled Air Particulate Matter SRM 1648a on the Development of Mild Collagen-Induced Arthritis in DBA/J Mice

“…To our knowledge, it is the first successful experimental evidence showing the arthritogenic effect of inhaled air particulate matter on the progression of CIA. Previously, the impact of cigarette smoke and diesel exhaust particles has been evaluated in a similar model (Kang et al 2020;Yoshino and Sagai 1999;Yoshino et al 2002). Establishment of the mild CIA is crucial for furthers studies of the risk factors that might possibly contribute to acceleration or aggravation of rheumatoid arthritis.…”

Modifiable risk factors linked to the development of rheumatoid arthritis: evidence, immunological mechanisms and prevention

“…NETs themselves are decorated with citrullinated proteins ( 52 ) and may be responsible for autoreactivity to citrullinated histones in RA patients ( 53 ). Some mouse models of RA have corroborated the effects of cigarette smoke exposure on inflammatory arthritis ( 54 ) and ACPA development ( 55 ). It should be noted however cigarette smoke has been shown to reduce CIA arthritis in some studies ( 56 ), a finding that is likely due to the timing of smoke exposure ( 57 ).…”

Identified GNGT1 and NMU as Combined Diagnosis Biomarker of Non-Small-Cell Lung Cancer Utilizing Bioinformatics and Logistic Regression

“…Cigarette smoking is a well-known risk factor for the occurrence and progression of malignant diseases [ 82 – 85 ]. Nicotine, the major constituent in cigarette smoke, plays key roles in cancer progression [ 86 – 89 ].…”