Exacerbating effects of trimellitic anhydride in ovalbumin-induced asthmatic mice and the gene and protein expressions of TRPA1, TRPV1, TRPV2 in lung tissue

Li, Mengwen; Fan, Xianqun; Ji, Leilei; Yang, Fan; Xu, Lina

doi:10.1016/j.intimp.2019.01.038

Cited by 17 publications

(9 citation statements)

References 36 publications

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“…Both TRPV1 and TRPV2 interact with phytocannabinoids, including CBD, CBG and THCV 19 . Also, TRPV1, TRPV2 and TRPA1 were found to be associated with pulmonary inflammation 20 www.nature.com/scientificreports/ F CBD:std activity. Only co-treatment with CB2 IA affected F CBD:std activity on IL-8 secretion.…”

Section: Discussionmentioning

confidence: 99%

“…Both TRPV1 and TRPV2 interact with phytocannabinoids, including CBD, CBG and THCV 19 . Also, TRPV1, TRPV2 and TRPA1 were found to be associated with pulmonary inflammation 20 . Nevertheless, cotreatment with CB1 IA, TRPA1 blocker or TRPV1 or TRPV2 antagonist had no substantial effect on F CBD and www.nature.com/scientificreports/ F CBD:std activity.…”

Section: Discussionmentioning

confidence: 99%

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Cannabis compounds exhibit anti-inflammatory activity in vitro in COVID-19-related inflammation in lung epithelial cells and pro-inflammatory activity in macrophages

Anil

Shalev

Vinayaka

et al. 2021

Sci Rep

111

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Cannabis sativa is widely used for medical purposes and has anti-inflammatory activity. This study intended to examine the anti-inflammatory activity of cannabis on immune response markers associated with coronavirus disease 2019 (COVID-19) inflammation. An extract fraction from C. sativa Arbel strain (FCBD) substantially reduced (dose dependently) interleukin (IL)-6 and -8 levels in an alveolar epithelial (A549) cell line. FCBD contained cannabidiol (CBD), cannabigerol (CBG) and tetrahydrocannabivarin (THCV), and multiple terpenes. Treatments with FCBD and a FCBD formulation using phytocannabinoid standards (FCBD:std) reduced IL-6, IL-8, C–C Motif Chemokine Ligands (CCLs) 2 and 7, and angiotensin I converting enzyme 2 (ACE2) expression in the A549 cell line. Treatment with FCBD induced macrophage (differentiated KG1 cell line) polarization and phagocytosis in vitro, and increased CD36 and type II receptor for the Fc region of IgG (FcγRII) expression. FCBD treatment also substantially increased IL-6 and IL-8 expression in macrophages. FCBD:std, while maintaining anti-inflammatory activity in alveolar epithelial cells, led to reduced phagocytosis and pro-inflammatory IL secretion in macrophages in comparison to FCBD. The phytocannabinoid formulation may show superior activity versus the cannabis-derived fraction for reduction of lung inflammation, yet there is a need of caution proposing cannabis as treatment for COVID-19.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cannabis compounds exhibit anti-inflammatory activity in vitro in COVID-19-related inflammation in lung epithelial cells and pro-inflammatory activity in macrophages

Anil

Shalev

Vinayaka

et al. 2021

Sci Rep

111

View full text Add to dashboard Cite

show abstract

“…In a gene-expression study of childhood asthma induced by three allergens (spring pollen, dust mite, dog and cat hair) in North China, a 2.6-fold increase in TRPV2-mRNA in lymphocytes was revealed [ 74 ]. These results were confirmed in an OVA mouse model, where TRPA1 and TRPV1 gene expression was also up-regulated ([ 75 ] reviewed in [ 76 ]). The exact role of this channel, which is further described in Section 3.2 , in this setting, however, remains elusive.…”

Section: Cells and Their Trp Expression In The Trachea And Bronchimentioning

confidence: 67%

Transient Receptor Potential (TRP) Channels in Airway Toxicity and Disease: An Update

Müller¹,

Alt²,

Rajan³

et al. 2022

Cells

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Our respiratory system is exposed to toxicants and pathogens from both sides: the airways and the vasculature. While tracheal, bronchial and alveolar epithelial cells form a natural barrier in the airways, endothelial cells protect the lung from perfused toxic compounds, particulate matter and invading microorganism in the vascular system. Damages induce inflammation by our immune response and wound healing by (myo)fibroblast proliferation. Members of the transient receptor potential (TRP) superfamily of ion channel are expressed in many cells of the respiratory tract and serve multiple functions in physiology and pathophysiology. TRP expression patterns in non-neuronal cells with a focus on TRPA1, TRPC6, TRPM2, TRPM5, TRPM7, TRPV2, TRPV4 and TRPV6 channels are presented, and their roles in barrier function, immune regulation and phagocytosis are summarized. Moreover, TRP channels as future pharmacological targets in chronic obstructive pulmonary disease (COPD), asthma, cystic and pulmonary fibrosis as well as lung edema are discussed.

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“…Therapeutics targeting these pathways are both showing promising results in the clinic (5,58,59) and have been associated with elevated neutrophils in severe steroidresistant asthma (127). Similarly, FGF C up-regulated TRPA1 and TRPV2, both of which have important functions in neurogenic airway inflammation and bronchoconstriction in asthma (128)(129)(130). Whether steroid-driven FGFs contribute to IL33, TSLP, TRPA1, or TRPV2 expression in individuals with severe uncontrolled asthma is unclear, but it is tempting to speculate that these non-type 2 pathways are aggravated by steroid-driven FGFs, reshaping the disease phenotype.…”

Section: Discussionmentioning

confidence: 99%

Steroid-induced fibroblast growth factors drive an epithelial-mesenchymal inflammatory axis in severe asthma

Guidi¹,

Xu²,

Choy³

et al. 2022

Sci. Transl. Med.

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Asthma and inflammatory airway diseases restrict airflow in the lung, compromising gas exchange and lung function. Inhaled corticosteroids (ICSs) can reduce inflammation, control symptoms, and improve lung function; however, a growing number of patients with severe asthma do not benefit from ICS. Using bronchial airway epithelial brushings from patients with severe asthma or primary human cells, we delineated a corticosteroid-driven fibroblast growth factor (FGF)–dependent inflammatory axis, with FGF-responsive fibroblasts promoting downstream granulocyte colony-stimulating factor (G-CSF) production, hyaluronan secretion, and neutrophilic inflammation. Allergen challenge studies in mice demonstrate that the ICS, fluticasone propionate, inhibited type 2–driven eosinophilia but induced a concomitant increase in FGFs, G-CSF, hyaluronan, and neutrophil infiltration. We developed a model of steroid-induced neutrophilic inflammation mediated, in part, by induction of an FGF-dependent epithelial-mesenchymal axis, which may explain why some individuals do not benefit from ICS. In further proof-of-concept experiments, we found that combination therapy with pan-FGF receptor inhibitors and corticosteroids prevented both eosinophilic and steroid-induced neutrophilic inflammation. Together, these results establish FGFs as therapeutic targets for severe asthma patients who do not benefit from ICS.

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Exacerbating effects of trimellitic anhydride in ovalbumin-induced asthmatic mice and the gene and protein expressions of TRPA1, TRPV1, TRPV2 in lung tissue

Cited by 17 publications

References 36 publications

Cannabis compounds exhibit anti-inflammatory activity in vitro in COVID-19-related inflammation in lung epithelial cells and pro-inflammatory activity in macrophages

Cannabis compounds exhibit anti-inflammatory activity in vitro in COVID-19-related inflammation in lung epithelial cells and pro-inflammatory activity in macrophages

Transient Receptor Potential (TRP) Channels in Airway Toxicity and Disease: An Update

Steroid-induced fibroblast growth factors drive an epithelial-mesenchymal inflammatory axis in severe asthma

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