Ex vivo study of bevacizumab transport through porcine nasal mucosa

Intranasal bevacizumab in the treatment of HHT -related epistaxis: a systematic review

“…No studies comprehensively accounted for patients' previous surgeries that may have influenced drug absorption. Despite this, it has been shown in ex vivo porcine nasal mucosal models that the majority of delivered bevacizumab could be retrieved either within or across the mucosal barrier suggesting that the local bioavailability of the drug is good (30) . In further support of local administration, diluted bevacizumab has been deemed stable during prolonged refrigerated storage in polyethylene bottles and this would therefore not be responsible for the hypothesized poor pharmacokinetics and/or bioavailability of the drug (31) .…”

Effect of Bevacizumab Nasal Spray on Epistaxis Duration in Hereditary Hemorrhagic Telangectasia

“…Bevacizumab transport through porcine nasal mucosa was evaluated and evidence of absorption was observed. 7 Furthermore, several published cases reported the potential efficacy of a bevacizumab nasal spray. [8][9][10][11][12] A phase 1 study 13 concluded that bevacizumab administered by nasal spray in HHT was safe regardless of dose.…”

ELLIPSE Study

“…Our results show that intranasal bevacizumab was very well tolerated immediately, 30 and 90 d after a one-day nasal spray administration, regardless of the dose. Preclinical studies have previously suggested that bevacizumab was well tolerated by the mucosa, 10 as have retrospective studies. 15 For safety reasons, however, it was deemed appropriate to begin with a single dose to test for tolerance.…”

“…Ex vivo studies showed that a large amount of the antibody was able to penetrate and cross the porcine nasal cavity mucosa. 10 The detection limit of the ELISA technique used to measure bevacizumab serum concentrations was 0.033 mg/L. 17 Bevacizumab was used without dilution and it has been shown that the drug is very stable, even after storage, making possible use in a nasal spray.…”

“…We therefore investigated bevacizumab transport through porcine nasal mucosa to determine antibody bioavailability and we observed evidence of absorption of bevacizumab into nasal mucosa. 10 Furthermore, several published cases reported a potential efficacy of bevacizumab nasal spray. [11][12][13][14][15] Our primary aim in the study reported here was to evaluate the safety in humans of a bevacizumab nasal spray.…”