Ex-Vivo Recording of LEUS-Generated Neural Responses from Mouse Brain Slices Using a Microelectrode Array (MEA) System

Castellanos, Ivan Suarez; N’Djin, W. Apoutou; Vion-Bailly, Jérémy; Perier, Magali; Huberfeld, Gilles; Birer, Alain; Carpentier, Alexandre; Chapelon, Jean‐Yves

doi:10.1109/ultsym.2018.8579847

Cited by 2 publications

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“…Recorded responses were shown to depend on neuronal activities since blocking voltage-gated sodium channels sustaining action potentials suppressed the response, as previously reported (Suarez-Castellanos et al, 2018). In our analysis, we found that the FUS-generated LFPs were entirely suppressed after 12 min of perfusion with TTX-supplemented aCSF as evidenced by the disappearance of the FUS-elicited fEPSP, thus suggesting that FUS-induced fEPSP are synaptically evoked.…”

Section: Discussionsupporting

confidence: 85%

Spatio-temporal characterization of causal electrophysiological activity stimulated by single pulse focused ultrasound: an ex vivo study on hippocampal brain slices

Castellanos¹,

Dossi²,

Vion-Bailly³

et al. 2021

J. Neural Eng.

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Objective. The brain operates via generation, transmission and integration of neuronal signals and most neurological disorders are related to perturbation of these processes. Neurostimulation by focused ultrasound (FUS) is a promising technology with potential to rival other clinically used techniques for the investigation of brain function and treatment of numerous neurological diseases. The purpose of this study was to characterize spatial and temporal aspects of causal electrophysiological signals directly stimulated by short, single pulses of FUS on ex vivo mouse hippocampal brain slices. Approach. Microelectrode arrays (MEAs) are used to study the spatio-temporal dynamics of extracellular neuronal activities both at the single neuron and neural networks scales. Hence, MEAs provide an excellent platform for characterization of electrical activity generated, modulated and transmitted in response to FUS exposure. In this study, a novel mixed FUS/MEA platform was designed for the spatio-temporal description of the causal responses generated by single 1.78 MHz FUS pulses in ex vivo mouse hippocampal brain slices. Main results. Our results show that FUS pulses can generate local field potentials (LFPs), sustained by synchronized neuronal post-synaptic potentials, and reproducing network activities. LFPs induced by FUS stimulation were found to be repeatable to consecutive FUS pulses though exhibiting a wide range of amplitudes (50–600 μV), durations (20–200 ms), and response delays (10–60 ms). Moreover, LFPs were spread across the hippocampal slice following single FUS pulses thus demonstrating that FUS may be capable of stimulating different neural structures within the hippocampus. Significance. Current knowledge on neurostimulation by ultrasound describes neuronal activity generated by trains of repetitive ultrasound pulses. This novel study details the causal neural responses produced by single-pulse FUS neurostimulation while illustrating the distribution and propagation properties of this neural activity along major neural pathways of the hippocampus.

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Section: Discussionsupporting

confidence: 85%

Spatio-temporal characterization of causal electrophysiological activity stimulated by single pulse focused ultrasound: an ex vivo study on hippocampal brain slices

Castellanos¹,

Dossi²,

Vion-Bailly³

et al. 2021

J. Neural Eng.

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“…Despite this limitation, some researchers have achieved those recordings [ 83 , 87 , 97 ]. LIFUS (15-30 mW/cm 2 ) increases the firing rate of CA1 hippocampal neurons by inhibiting K + currents [ 83 ] (Fig.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Potential of Ultrasound Neuromodulation in Decreasing Neuropathic Pain: Clinical and Experimental Evidence

Pérez-Neri

González-Aguilar

Sandoval

et al. 2021

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Background: For more than seven decades, ultrasound has been used as an imaging and diagnostic tool. Today, new technologies - as focused ultrasound (FUS) neuromodulation - has revealed some innovative, potential applications. However, those applications have been barely studied to deal with neuropathic pain (NP), a cluster of chronic pain syndromes with a restricted response to conventional pharmaceuticals. Objective: To analyze the therapeutic potential of low-intensity (LIFUS) and high-intensity (HIFUS) FUS for managing NP. Methods: We performed a narrative review, including clinical and experimental ultrasound neuromodulation studies published in three main database repositories. Discussion: Evidence shows that FUS may influence several mechanisms relevant for neuropathic pain managing as modulation of ion channels, glutamatergic neurotransmission, cerebral blood flow, inflammation and neurotoxicity, neuronal morphology and survival, nerve regeneration, and remyelination. Some experimental models have shown that LIFUS may reduce allodynia after peripheral nerve damage. At the same time, a few clinical studies support its beneficial effect on reducing pain in nerve compression syndromes. In turn, Thalamic HIFUS ablation can reduce NP from several etiologies with minor side-effects, but some neurological sequelae might be permanent. HIFUS is also effective useful in lowering non-neuropathic pain in several disorders. Conclusion: Although an emerging set of studies brings new evidence on the therapeutic potential of both LIFUS and HIFUS for managing NP with minor side-effects, we need more controlled clinical trials to conclude about its safety and efficacy.

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Ex-Vivo Recording of LEUS-Generated Neural Responses from Mouse Brain Slices Using a Microelectrode Array (MEA) System

Cited by 2 publications

References 0 publications

Spatio-temporal characterization of causal electrophysiological activity stimulated by single pulse focused ultrasound: an ex vivo study on hippocampal brain slices

Spatio-temporal characterization of causal electrophysiological activity stimulated by single pulse focused ultrasound: an ex vivo study on hippocampal brain slices

Therapeutic Potential of Ultrasound Neuromodulation in Decreasing Neuropathic Pain: Clinical and Experimental Evidence

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