Ex vivo/in vitro absorption of STW 5 (Iberogast®) and its extract components

Kelber, O; Wittwer, Arthur J.; Lapke, C.; Kroll, U.; Weiser, D; Okpanyi, S. N.; Heilmann, Jörg

doi:10.1016/j.phymed.2006.07.002

Cited by 12 publications

(7 citation statements)

References 26 publications

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“…We believe that our result have functional relevance as the smallest effective concentration was 200th of one therapeutic serving. Moreover, besides local actions of STW 5 in the colon an additional systemic effect appears likely 10 . Preliminary reports demonstrating efficacy of STW 5 in treatment of experimental colitis would support a systemic mode of action 28,29 .…”

Section: Discussionmentioning

confidence: 94%

“…Moreover, besides local actions of STW 5 in the colon an additional systemic effect appears likely. 10 Preliminary reports demonstrating efficacy of STW 5 in treatment of experimental colitis would support a systemic mode of action. 28,29 Based on our results in the Ussing chamber, topical and systemic actions are conceivable as apical and basolateral application were effective.…”

Section: Discussionmentioning

confidence: 98%

“…Studies in experimental models demonstrated a broad action profile of STW 5 including modulation of muscle activity in the stomach, 4 radical scavenging, 7 anti‐inflammatory 8 and antioxidative properties 9 . These actions very likely depend on local and systemic effects of STW 5 supported by the good bioavailability of lead substances characteristic for each of the extracts 10 . As for other multi‐herbal drug preparations, the concept of multitargeting may improve treatment options for gut diseases that are associated with multifactorial disorders 11 .…”

Section: Introductionmentioning

confidence: 99%

“…9 These actions very likely depend on local and systemic effects of STW 5 supported by the good bioavailability of lead substances characteristic for each of the extracts. 10 As for other multi-herbal drug preparations, the concept of multitargeting may improve treatment options for gut diseases that are associated with multifactorial disorders. 11 This is supported by clinical studies, which demonstrated that STW 5 improved the overall symptom score in irritable bowel (IBS) patients.…”

Section: Introductionmentioning

confidence: 99%

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The multi‐herbal drug STW 5 (Iberogast^®) has prosecretory action in the human intestine

Krueger

Gruber

Bühner

et al. 2009

Neurogastroenterology Motil

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There is growing evidence that STW 5 (Iberogast), fixed combination of hydroethanolic herbal extracts), besides being effective in functional dyspepsia, also improves symptoms in irritable bowel syndrome (IBS). Clinical data indicate that modulation of mucosal secretion is a promising approach to treat intestinal disorders associated with IBS. We therefore explored the effect of STW 5 on secretion in the human intestine and the mechanisms by which it acts. The Ussing chamber technique was used to measure mucosal secretion in human intestinal mucosa/submucosa preparations and in human epithelial cell line T84. In addition, we recorded STW 5 effects on human enteric neurons with voltage sensitive dye imaging. In human tissue and T84 cells STW 5 induced a dose-dependent increase in ion secretion that was significantly reduced by the Na-K-Cl cotransporter blocker bumetanide, the adenylate cyclase inhibitor MDL-12 330, the non-specific and selective cystic fibrosis transmembrane conductance regulator (CFTR) inhibitors glibenclamide and CFTR(inh)-172, respectively, and the blocker of calcium dependent Cl(-) channels (ClCa) SITS (4-acetamido-4-isothiocyanatostilbene-2,2-disulphonic acid). It was unaffected by amiloride, a blocker of epithelial Na(+) channels. In human tissue, the nerve blocker tetrodotoxin significantly suppressed the STW 5 response. STW 5 evoked an increased spike discharge in 51% of human submucous neurons. Results suggest that STW 5 is a secretogogue in the human intestine by direct epithelial actions and through activation of enteric neurons. The prosecretory effect is due to increased epithelial Cl(-) fluxes via CFTR and Ca-dependent ClCa channels. STW 5 may be a novel option to treat secretory disorders associated with IBS and constipation.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The multi‐herbal drug STW 5 (Iberogast^®) has prosecretory action in the human intestine

Krueger

Gruber

Bühner

et al. 2009

Neurogastroenterology Motil

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“…The low volume of the absorption compartment, in relation of the absorption surface of the gut and the large external drug depot, preventing a shift in drug concentrations in the external medium during the experiments, is also an advantage of this model. The everted gut sac model has been used for quantitative information about resorption of drugs like phytopharmaceuticals (32), toxins (33) or for the assessment of co-factors affecting the bioavailability of other drugs (34).…”

Section: Discussionmentioning

confidence: 99%

Pharmacokinetic aspects of reduced nicotinamide adenin dinucleotide (NADH) in rats

Rex

Fink²

2008

Front Biosci

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Nicotinamide adenine dinucleotide (NADH) plays a major role in cellular metabolism and mitochondrial dysfunction and is thought that NAD+/NADH decrease neuronal degeneration and improve behavioral deficits. This potential use of NAD+ or NADH as neuroprotective drugs requires an insight on the pharmacokinetic properties of these compounds. For this reason, we assessed the absorption of NADH in the small intestine in vitro using the everted gut sac technique. We show an enteral absorption of the intact NADH molecule. In the gut sac, NADH had a concentration-independent absorption rate of about 5 percent and the in vivo laser-induced fluorescence spectroscopy revealed a relatively quick absorption of NADH starting after a few minute reaching a plateau (about 5 percent ) after 20-30 minutes. Theses results show that, should NADH be protected against the acidic conditions of the stomach, NADH is absorbed principally in the small intestine.

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Adenosine A2A receptor contributes to the anti-inflammatory effect of the fixed herbal combination STW 5 (Iberogast®) in rat small intestinal preparations

Michael

Abdel-Aziz²,

Weiser³

et al. 2011

Naunyn-Schmiedeberg's Arch Pharmacol

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STW 5 (Iberogast®), an established herbal combination, was effective in randomized, double blind clinical studies in functional dyspepsia and irritable bowel syndrome. Since STW 5 was found to influence intestinal motility and has anti-inflammatory properties, this study investigated the expression of adenosine receptors and characterized their role in the control of the anti-inflammatory action of STW 5 and its fresh plant component STW 6 in inflammation-disturbed rat small intestinal preparations. The inflammation was induced by intraluminal instillation of 2,4,6-trinitrobenzene sulfonic acid (TNBS, 0.01 M). The effects of coincubation with selective receptor agonists and antagonists, STW 5, STW 6, or combinations of these compounds on acetylcholine (ACh)-evoked contraction of ileum/jejunum preparations were tested. Adenosine receptor mRNA expression was examined by reverse transcription-polymerase chain reaction (RT-PCR). In untreated preparations, RT-PCR revealed the presence of all adenosine receptor subtypes. Suppressed expression was detected for all subtypes in inflamed tissues, except for A(2B)R mRNA, which was unaffected. STW 5 reversed these effects and enhanced A(2A)R expression above control levels. Radioligand binding assays confirm the affinity of STW 5 to the A(2A)R, and the A(2A)R antagonist was able to prevent the effect of STW 5 on TNBS-induced attenuation of the ACh contraction. Our findings provide evidence that STW 5, but not STW 6 interacts with A(2A)R, which is involved in the anti-inflammatory action of STW 5. STW 6 did not contribute to adenosine A(2A)R-mediated anti-inflammatory effect of STW 5. Other signaling pathways could be involved in the mechanism of action of STW 6.

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Ex vivo/in vitro absorption of STW 5 (Iberogast®) and its extract components

Cited by 12 publications

References 26 publications

The multi‐herbal drug STW 5 (Iberogast^®) has prosecretory action in the human intestine

The multi‐herbal drug STW 5 (Iberogast^®) has prosecretory action in the human intestine

Pharmacokinetic aspects of reduced nicotinamide adenin dinucleotide (NADH) in rats

Adenosine A2A receptor contributes to the anti-inflammatory effect of the fixed herbal combination STW 5 (Iberogast®) in rat small intestinal preparations

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Ex vivo/in vitro absorption of STW 5 (Iberogast®) and its extract components

Cited by 12 publications

References 26 publications

The multi‐herbal drug STW 5 (Iberogast®) has prosecretory action in the human intestine

The multi‐herbal drug STW 5 (Iberogast®) has prosecretory action in the human intestine

Pharmacokinetic aspects of reduced nicotinamide adenin dinucleotide (NADH) in rats

Adenosine A2A receptor contributes to the anti-inflammatory effect of the fixed herbal combination STW 5 (Iberogast®) in rat small intestinal preparations

Contact Info

Product

Resources

About

The multi‐herbal drug STW 5 (Iberogast^®) has prosecretory action in the human intestine

The multi‐herbal drug STW 5 (Iberogast^®) has prosecretory action in the human intestine