Ex vivo decontamination of yeast-colonized dentures by iodine&amp;ndash;thiocyanate complexes

Sebaa, Sarra; Faltot, Maxime; Breucker, Sandra De; Boucherit-Otmani, Z.; Bafort, Françoise; Perraudin, J. P.; Courtois, Philippe

doi:10.2147/ccide.s165377

Cited by 4 publications

(5 citation statements)

References 24 publications

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“…[9,10]. While studying biological lactoperoxidase activity in the presence of an SCN − /I − mixture, a few articles have addressed the products generated under such and such experimental conditions [11,12,21,27]; several have showed that a more stable oxidative complex was formed in specific pH and ionic strength conditions [25,[27][28][29]. We found OSCN − after LPO oxidation of SCN − and I 2 after LPO oxidation of I − .…”

Section: Influence Of the Substrate Concentrations On Antifungal Actimentioning

confidence: 70%

Effect of the Nature and Relative Concentration of Substrate, Water Mineralization, and Storage Temperature on the Oxidants Produced by Lactoperoxidase and on Their Antifungal Activity against Penicillium expansum and Botrytis cinerea

et al. 2019

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Lactoperoxidase is an enzyme that generates oxidants with antimicrobial activity in presence of a (pseudo)halogen and hydrogen peroxide, but various factors can drastically reduce the antimicrobial activity of the lactoperoxidase system. Spectroscopic, ionic chromatography, and 13C-NMR methods showed that the oxidants generated by lactoperoxidase are OSCN− in the presence of SCN− and I2 in the presence of I−. Neither of them, however, inhibited Penicillium expansum, one of the causal agents of fruit mold. When a mixture of SCN− and I− was used, no OSCN−, OCN−, I2, or interhalogen I2SCN− was produced. However, its long-term stability, NH2-oxidizing capacity, and antifungal activity against P. expansum argue in favor of an I−-derived oxidant. Strongly mineralized water optimized enzyme-catalyzed reactions with higher oxidant production. Storage at 4 °C resulted in long-term stability and extended antifungal activity against P. expansum. The relative iodide/thiocyanate concentrations turned out to be important, as better in vitro inhibition of Botrytis cinerea, the causal agent of apples’s grey mold, was obtained with a high KI + KSCN concentration, a KI/KSCN ratio of 4.5, and a (KI + KSCN)/H2O2 ratio of 1. The nature of the substrates, their relative concentrations, the medium, and the storage temperature modifed the antifungal activity of lactoperoxidase.

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Section: Influence Of the Substrate Concentrations On Antifungal Actimentioning

confidence: 70%

Effect of the Nature and Relative Concentration of Substrate, Water Mineralization, and Storage Temperature on the Oxidants Produced by Lactoperoxidase and on Their Antifungal Activity against Penicillium expansum and Botrytis cinerea

et al. 2019

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“…Human fungal infections including those caused by yeast and yeast-like fungi constitute a serious burden in Algeria [48][49][50][51][52][53][54][55][56][57]. At least 27 taxa are identified in care controls.…”

Section: Yeasts Within Human Healthmentioning

confidence: 99%

“…neoformans. Only one case mentioned a denture contaminated by C. albicans [57], the ex vivo decontamination of this yeastcolonized denture was realized by iodine-thiocyanate.…”

Section: Yeasts Within Human Healthmentioning

confidence: 99%

Inventoried Yeast Species in Algeria

Benkhalifa¹

2023

Biomedical Engineering

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In Algeria, the study of yeasts remained marginalized for a long time. One of the supposed causes would be the reduction of examples in the school system. In medicine, species are identified because of their pathogenicity. But in food production and other fields, yeasts are mentioned as quantification in the quality-control process as well as molds. In addition to homemade breads, traditions of fermentation involving yeasts are the process of other local products, such as dairy products, vinegars, beverages, and authentic cheeses. Yeasts affect fruits and plants but also increase yields and protect species from other pathogenic microorganisms. Some conscious researchers have looked into the prospecting of yeast showing their properties and evaluating their interest, adopting biotechnology approaches, and covering several environments. 131 taxa are listed in this first compilation with 27 species in human health, 30 in animal health, 27 from dairy products, 24 taxa isolated from soils, 41 from agricultural products, and 17 concerned environmental purposes. Biotechnologies concern 44 taxa in varied topics of biomass, ethanol, vinegar, enzymes, and esters. Sixteen selected natural products inhibit 14 yeast species. Many isolated strains are promising in agriculture, agri-food, and biotechnologies and present new economic prospects. The idea of national depository is proposed.

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“…Mixtures of the two substrates, SCN − and I − , however, appear to lead to the formation of a more stable oxidative complex over time ( 65 , 66 , 87 – 90 ). Under well-defined in vitro experimental conditions (high ionic strength; KI:KSCN ratio, ~4.5; neutral or acidic buffer; presence of H 2 O 2 ), lactoperoxidase produces stable iodine-SCN − complexes, such as I 2 (SCN) − , objectified by carbon-13 (C13) nuclear magnetic resonance spectroscopy ( 66 ) with antimicrobial properties against Candida ( 67 ). Oxidant concentrations are then less toxic to epithelial cells in the mouth, as compared with chlorhexidine ( 67 ).…”

Section: Oxidant Production By Oral Peroxidasesmentioning

confidence: 99%

“…Under well-defined in vitro experimental conditions (high ionic strength; KI:KSCN ratio, ~4.5; neutral or acidic buffer; presence of H 2 O 2 ), lactoperoxidase produces stable iodine-SCN − complexes, such as I 2 (SCN) − , objectified by carbon-13 (C13) nuclear magnetic resonance spectroscopy ( 66 ) with antimicrobial properties against Candida ( 67 ). Oxidant concentrations are then less toxic to epithelial cells in the mouth, as compared with chlorhexidine ( 67 ). The resistance of epithelial cells may be explained by the presence of bacteria covering their surface and forming a protective biofilm, and/or by the detoxification of enzymatic activities in certain commensal species present in the oral environment ( 91 ).…”

Section: Oxidant Production By Oral Peroxidasesmentioning

confidence: 99%

Oral peroxidases: From antimicrobial agents to ecological actors (Review)

Courtois

2021

Mol Med Rep

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Sialoperoxidase and myeloperoxidase are the two main peroxidase enzymes found in the oral cavity. Sialoperoxidase is present in salivary secretions and in the biofilms that line the oral surfaces, while myeloperoxidase is abundant in the dento-gingival sulcus area. In the presence of hydrogen peroxide (H 2 O 2 ), oral peroxidases catalyze the oxidation of the pseudohalide anion thiocyanate (SCN − ) to hypothiocyanite (OSCN − ), a strong oxidant that serves an antimicrobial role. Furthermore, oral peroxidases consume bacteria-produced H 2 O 2 and could help inactivate toxic carcinogenic and genotoxic substances. Numerous in vitro studies have reported the antibacterial, antimycotic and antiviral role of peroxidases, suggesting possible applications in oral therapy. However, the use of oral hygiene products incorporating peroxidase systems has not yet been shown to be beneficial for the treatment or prevention of oral infections. This paradox reflects our incomplete knowledge of the physiological role of peroxidases in a complex environment, such as the oral region. While hygiene is crucial for restoring oral microbiota to a symbiotic state, there are no data to suggest that the addition of a peroxidase per se can create a dysbiotic state. Recent investigations have associated the presence of peroxidase activity with gram-positive cocci microbial flora, and its insufficiency with dysbiosis has been linked to pathologies, such as caries, periodontitis or infections of the oral mucosa. Therefore, oxidants generated by oral peroxidases appear to be an essential ecological determinant for oral health through the selection of a symbiotic microbiota capable of resisting oxidative stress. The objective of the present review was to update the current knowledge of the physiological aspects and applications of oral peroxidases in clinical practice.

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Ex vivo decontamination of yeast-colonized dentures by iodine–thiocyanate complexes

Cited by 4 publications

References 24 publications

Effect of the Nature and Relative Concentration of Substrate, Water Mineralization, and Storage Temperature on the Oxidants Produced by Lactoperoxidase and on Their Antifungal Activity against Penicillium expansum and Botrytis cinerea

Effect of the Nature and Relative Concentration of Substrate, Water Mineralization, and Storage Temperature on the Oxidants Produced by Lactoperoxidase and on Their Antifungal Activity against Penicillium expansum and Botrytis cinerea

Inventoried Yeast Species in Algeria

Oral peroxidases: From antimicrobial agents to ecological actors (Review)

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