Ex-situ conservation and morpho-biochemical study of exotic germplasm of potato

Khan, Arifa S.; Erum, Shazia; Riaz, Naveeda; Shinwari, Muhammad Ibrar; Shinwari, Maryum Ibrar

doi:10.30848/pjb2022-3(39)

Cited by 2 publications

(2 citation statements)

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“…Specifically, in the presence of mannitol, the highest levels of mortality are observed, supporting similar observations in Uncaria tomentosa (Cruz-Mora et al, 2011) and Arnica montana (Petrova et al, 2020), where tissue oxidation and death are manifested. A determining factor in mortality is the concentration of the osmotic substance, especially in the case of mannitol; high concentrations are associated with increases in mortality, consistent with similar findings reported by Bello-Bello et al ( 2014) and Khan et al (2022). At the end of the storage period, sucrose proves to be the osmotic substance with the lowest percentage of mortality, followed by sorbitol.…”

Section: Mortalitysupporting

confidence: 88%

“…5D; 6B). In general, the inclusion of osmotic substances such as sucrose, mannitol (Silva and Scherwinski-Pereira, 2011;Akdemir et al, 2013), andsorbitol (Díaz-Narváez et al, 2015;Elhomosany and El-Wahab, 2020;Petrova et al, 2020;Benelli et al, 2022;Khan et al, 2022), as well as the reduction of nutrient concentration (Mohanty et al, 2013;Castilla-Valdés et al, 2020) in the culture medium, influence the subculturing interval and storage time.…”

Section: Mortalitymentioning

confidence: 99%

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Cinchona officinalis L. in vitro conservation by means of slow-growth storage and cryopreservation techniques

Armijos-Gonzalez,

Ramón-Contento,

Cueva-Agila

2023

Preprint

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Cinchona officinalis has experienced anthropogenic pressures for nearly 400 years, such as overexploitation, habitat fragmentation, and the subsequent reduction of genetic diversity. Additionally, the challenge of regeneration in its natural environment makes it a vulnerable species. In this context, various treatments for the in vitro conservation of explants were evaluated in the present study. For short-term conservation, the effects of osmotic substances such as sorbitol, mannitol, and sucrose at different concentrations were assessed. Different concentrations of MS and B5 culture media were also examined for their impact on the growth, budding, mortality, and rooting of explants over 12 months without subcultures. For long-term conservation by cryopreservation, two techniques were tested: vitrification and encapsulation-dehydration. Short-term preservation of explants in sorbitol resulted in low mortality, minimal growth, and limited development of new shoots compared to preservation in sucrose or mannitol, although tissues could be recovered successfully from all storage conditions. After cryopreservation and 45 days of recovery, explants with the lowest mortality (4%) were from the control treatment, cultivated in a medium with sucrose which proved useful as a cryoprotectant, followed by the encapsulation-dehydration treatment. In conclusion, it is possible to conserve C. officinalis tissues in the short-term using in vitro techniques, while further assays are needed for long-term conservation.

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Section: Mortalitysupporting

confidence: 88%