Ex-situ biodegradation of petroleum hydrocarbons using Alcanivorax borkumensis enzymes

Kadri, Tayssir; Magdouli, Sara; Rouissi, Tarek; Brar, Satinder Kaur

doi:10.1016/j.bej.2018.01.014

Cited by 41 publications

(12 citation statements)

References 47 publications

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“…The reduction of residual n-alkane content resulted in a decline of extracellular alkane monooxygenase activity due to less substrate bioavailability. A similar observation was reported by Olajuyigbe and Ehiosun (2016) and Kadri et al (2018a).…”

Section: Discussionsupporting

confidence: 91%

Microbial Biodegradation of Paraffin Wax in Malaysian Crude Oil Mediated by Degradative Enzymes

et al. 2020

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The deposition of paraffin wax in crude oil is a problem faced by the oil and gas industry during extraction, transportation, and refining of crude oil. Most of the commercialized chemical additives to prevent wax are expensive and toxic. As an environmentally friendly alternative, this study aims to find a novel thermophilic bacterial strain capable of degrading paraffin wax in crude oil to control wax deposition. To achieve this, the biodegradation of crude oil paraffin wax by 11 bacteria isolated from seawater and oil-contaminated soil samples was investigated at 70 • C. The bacteria were identified as Geobacillus kaustophilus N3A7, NFA23, DFY1, Geobacillus jurassicus MK7, Geobacillus thermocatenulatus T7, Parageobacillus caldoxylosilyticus DFY3 and AZ72, Anoxybacillus geothermalis D9, Geobacillus stearothermophilus SA36, AD11, and AD24. The GCMS analysis showed that strains N3A7, MK7, DFY1, AD11, and AD24 achieved more than 70% biodegradation efficiency of crude oil in a short period (3 days). Notably, most of the strains could completely degrade C 37-C 40 and increase the ratio of C 14-C 18 , especially during the initial 2 days incubation. In addition, the degradation of crude oil also resulted in changes in the pH of the medium. The degradation of crude oil is associated with the production of degradative enzymes such as alkane monooxygenase, alcohol dehydrogenase, lipase, and esterase. Among the 11 strains, the highest activities of alkane monooxygenase were recorded in strain AD24. A comparatively higher overall alcohol dehydrogenase, lipase, and esterase activities were observed in strains N3A7, MK7, DFY1, AD11, and AD24. Thus, there is a potential to use these strains in oil reservoirs, crude oil processing, and recovery to control wax deposition. Their ability to withstand high temperature and produce degradative enzymes for long-chain hydrocarbon degradation led to an increase in the short-chain hydrocarbon ratio, and subsequently, improving the quality of the oil.

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Section: Discussionsupporting

confidence: 91%

Microbial Biodegradation of Paraffin Wax in Malaysian Crude Oil Mediated by Degradative Enzymes

et al. 2020

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“…SZN1 and Alcanivorax sp. SZN2 belong to genera already described as capable of degrading hydrocarbons [31,32], they do not cluster closely with species with hydrocarbon degrading activity. Similarly, Pseudoalteromonas sp.…”

Section: Heavy Metal Immobilizationmentioning

confidence: 88%

Degradation of Hydrocarbons and Heavy Metal Reduction by Marine Bacteria in Highly Contaminated Sediments

et al. 2020

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Investigations on the ability of bacteria to enhance removal of hydrocarbons and reduce heavy metal toxicity in sediments are necessary to design more effective bioremediation strategies. In this study, five bacterial strains, Halomonas sp. SZN1, Alcanivorax sp. SZN2, Pseudoalteromonas sp. SZN3, Epibacterium sp. SZN4, and Virgibacillus sp. SZN7, were isolated from polluted sediments from an abandoned industrial site in the Gulf of Naples, Mediterranean Sea, and tested for their bioremediation efficiency on sediment samples collected from the same site. These bacteria were added as consortia or as individual cultures into polluted sediments to assess biodegradation efficiency of polycyclic aromatic hydrocarbons and heavy metal immobilisation capacity. Our results indicate that these bacteria were able to remove polycyclic aromatic hydrocarbons, with a removal rate up to ca. 80% for dibenzo-anthracene. In addition, these bacteria reduced arsenic, lead, and cadmium mobility by promoting their partitioning into less mobile and bioavailable fractions. Microbial consortia generally showed higher performance toward pollutants as compared with pure isolates, suggesting potential synergistic interactions able to enhance bioremediation capacity. Overall, our findings suggest that highly polluted sediments select for bacteria efficient at reducing the toxicity of hazardous compounds, paving the way for scaled-up bioremediation trials.

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“…Sua eficiência como catalisador pode ser explicada através da concentração de sua atividade (U/ml), sendo determinado a partir da velocidade de reação, ou seja, a atividade enzimática corresponde à quantidade de enzima que catalisa para a formação de produto por unidade de tempo (Costa, 2016). Para reações catalisadas, a velocidade de reação pode ser acelerada para a ordem de milhões de vezes, por exemplo, a enzima orotidina monofosfato descarboxilase diminui o tempo de reação por ela catalisada de 78 milhões de anos para 25 milissegundos (Kadri, et al 2018). Nos estudos dos processos enzimáticos, para remediação de sedimentos e solos contaminados por petróleo, a cinética tem importante destaque (Tan, et al 2021).…”

Section: Cinética Das Enzimasunclassified

Processos enzimáticos na biorremediação e fitorremediação de petróleo em sedimentos de manguezal: uma revisão

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Filho

et al. 2021

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Diversos vazamentos de petróleo em mares e oceanos ocorreram nas últimas décadas, afetando principalmente estuários e manguezais. Acidentes ambientais envolvendo o petróleo, seus derivados e os resíduos gerados têm sido uma preocupação crescente, por conta dos hidrocarbonetos do petróleo ser considerados contaminantes orgânicos tóxicos e de difícil degradação. Os avanços biotecnológicos têm levado os pesquisadores a adotar a biorremediação e fitorremediação como alternativas potencialmente eficazes para solos e sedimentos contaminados e o uso enzimas têm sido adotado como uma estratégia além do uso de plantas e microrganismos na biorremediação. Este estudo consiste em uma revisão bibliográfica baseada em livros, artigos, teses, dissertações e sites, com o intuito de reunir e avaliar os processos enzimáticos na fitorremediação e fitorremediação em ambientes impactados por atividades do petróleo.

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Ex-situ biodegradation of petroleum hydrocarbons using Alcanivorax borkumensis enzymes

Cited by 41 publications

References 47 publications

Microbial Biodegradation of Paraffin Wax in Malaysian Crude Oil Mediated by Degradative Enzymes

Microbial Biodegradation of Paraffin Wax in Malaysian Crude Oil Mediated by Degradative Enzymes

Degradation of Hydrocarbons and Heavy Metal Reduction by Marine Bacteria in Highly Contaminated Sediments

Processos enzimáticos na biorremediação e fitorremediação de petróleo em sedimentos de manguezal: uma revisão

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