Evolving treatment paradigms in recurrent and metastatic head and neck squamous cell carcinoma: the emergence of immunotherapy

Faraji, Farhoud; Cohen, Ezra E.W.; Guo, Theresa

doi:10.21037/tcr-23-1

Cited by 1 publication

(1 citation statement)

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“…Despite the use of multimodal therapy, the local-regional recurrence rate for advanced HNSCC can be as high as 50% [6]. Notably, recurrent and metastatic disease emerges as a major cause of mortality in HNSCC [7]. Despite remarkable advances in surgery, radiation, and medical therapy over the past few decades, the prognosis for HN-SCC remains poor, with a 5-year overall survival rate ranging from 25% for hypopharyngeal cancer to 59% for laryngeal cancer [8].…”

Section: Introductionmentioning

confidence: 99%

A Tissue Engineered 3D Model of Cancer Cell Invasion for Human Head and Neck Squamous-Cell Carcinoma

Stöth,

Mineif,

Sauer

et al. 2024

CIMB

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Head and neck squamous-cell carcinoma (HNSCC) is associated with aggressive local invasiveness, being a main reason for its poor prognosis. The exact mechanisms underlying the strong invasive abilities of HNSCC remain to be elucidated. Therefore, there is a need for in vitro models to study the interplay between cancer cells and normal adjacent tissue at the invasive tumor front. To generate oral mucosa tissue models (OMM), primary keratinocytes and fibroblasts from human oral mucosa were isolated and seeded onto a biological scaffold derived from porcine small intestinal submucosa with preserved mucosa. Thereafter, we tested different methods (single tumor cells, tumor cell spots, spheroids) to integrate the human cancer cell line FaDu to generate an invasive three-dimensional model of HNSCC. All models were subjected to morphological analysis by histology and immunohistochemistry. We successfully built OMM tissue models with high in vivo–in vitro correlation. The integration of FaDu cell spots and spheroids into the OMM failed. However, with the integration of single FaDu cells into the OMM, invasive tumor cell clusters developed. Between segments of regular epithelial differentiation of the OMM, these clusters showed a basal membrane penetration and lamina propria infiltration. Primary human fibroblasts and keratinocytes seeded onto a porcine carrier structure are suitable to build an OMM. The HNSCC model with integrated FaDu cells could enable subsequent investigations into cancer cell invasiveness.

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Section: Introductionmentioning

confidence: 99%