Evolved patterns and rates of water loss and ion regulation in laboratory-selected populations of<i>Drosophila melanogaster</i>

Folk, Donna G.; Bradley, Timothy J.

doi:10.1242/jeb.00498

Cited by 35 publications

(51 citation statements)

References 44 publications

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“…Drosophila melanogaster (Albers and Bradley, 2004;Folk and Bradley, 2003) and related Drosophila species (Gibbs and Matzkin, 2001;Hoffmann, 2003) have been the focus of most previous works on dipteran water balance. Studies with Drosophila demonstrate higher dehydration resistance (Gibbs and Matzkin, 2001;Matzkin et al, 2007), reduced water loss rates (Gibbs and Matzkin, 2001) and reduced metabolism in xeric-adapted species (Gibbs et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Repeated bouts of dehydration deplete nutrient reserves and reduce egg production in the mosquito Culex pipiens

Benoit

Patrick

Desai

et al. 2010

Journal of Experimental Biology

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SUMMARYIn this study of the mosquito, Culex pipiens, we examined the impact of multiple bouts of dehydration and rehydration on survival, depletion of metabolic reserves and egg production in both non-diapausing and diapausing females. Mosquitoes provided with access to sugar during rehydration survived longer than those allowed to rehydrate without sugar, and their survival was similar to that of mosquitoes of the same age that were not dehydrated. Among mosquitoes not provided with sugar, each dehydration bout reduced the mosquito's dry mass -an effect likely to be due to the utilization of carbohydrates and lipid reserves. The toll on glycogen and lipid reserves is likely to be especially costly for diapausing mosquitoes that are dependent on these stored reserves for winter survival. Egg production in both non-diapausing and post-diapausing C. pipiens was also reduced in response to multiple bouts of dehydration. Although egg quality was not compromised, the number of eggs produced was reduced. Both non-diapausing and diapausing females can compensate for the nutrient loss due to dehydration by sugar feeding but the opportunity to feed on sugar is likely to be rarely available in the overwintering habitat of diapausing females, thus the impact of dehydration may be especially pronounced in overwintering populations of C. pipiens.

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Section: Discussionmentioning

confidence: 99%

Repeated bouts of dehydration deplete nutrient reserves and reduce egg production in the mosquito Culex pipiens

Benoit

Patrick

Desai

et al. 2010

Journal of Experimental Biology

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“…In D. melanogaster, desiccation resistance is improved by decreasing the rate of water loss or by increasing the water content of the body (1,17,32), and under desiccation conditions desiccated flies with reduced capa levels retained markedly larger abdomens, indicating a potentially higher hemolymph volume, compared with parental controls (Fig. S6A).…”

Section: Capa Neuropeptides Are Released Not During Desiccation and Coldmentioning

confidence: 99%

Insect capa neuropeptides impact desiccation and cold tolerance

Terhzaz

Teets

Cabrero

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

107

128

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The success of insects is linked to their impressive tolerance to environmental stress, but little is known about how such responses are mediated by the neuroendocrine system. Here we show that the capability (capa) neuropeptide gene is a desiccation-and cold stressresponsive gene in diverse dipteran species. Using targeted in vivo gene silencing, physiological manipulations, stress-tolerance assays, and rationally designed neuropeptide analogs, we demonstrate that the Drosophila melanogaster capa neuropeptide gene and its encoded peptides alter desiccation and cold tolerance. Knockdown of the capa gene increases desiccation tolerance but lengthens chill coma recovery time, and injection of capa peptide analogs can reverse both phenotypes. Immunohistochemical staining suggests that capa accumulates in the capa-expressing Va neurons during desiccation and nonlethal cold stress but is not released until recovery from each stress. Our results also suggest that regulation of cellular ion and water homeostasis mediated by capa peptide signaling in the insect Malpighian (renal) tubules is a key physiological mechanism during recovery from desiccation and cold stress. This work augments our understanding of how stress tolerance is mediated by neuroendocrine signaling and illustrates the use of rationally designed peptide analogs as agents for disrupting protective stress tolerance.environmental stress | insects | neuropeptides | capa | desiccation and cold tolerance

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“…The Journal of Experimental Biology (2014) doi:10.1242/jeb.090571 reduced in desiccated D. melanogaster (Folk and Bradley, 2003), and work with wild desiccation-resistant Drosophila populations showed single-feature polymorphisms in several gene groups. These included tubule ion transport genes, e.g.…”

Section: Reviewmentioning

confidence: 99%

Cell signalling mechanisms for insect stress tolerance

Davies

Cabrero

Overend

et al. 2014

Journal of Experimental Biology

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Insects successfully occupy most environmental niches and this success depends on surviving a broad range of environmental stressors including temperature, desiccation, xenobiotic, osmotic and infection stress. Epithelial tissues play key roles as barriers between the external and internal environments and therefore maintain homeostasis and organismal tolerance to multiple stressors. As such, the crucial role of epithelia in organismal stress tolerance cannot be underestimated. At a molecular level, multiple cell-specific signalling pathways including cyclic cAMP, cyclic cGMP and calcium modulate tissue, and hence, organismal responses to stress. Thus, epithelial cell-specific signal transduction can be usefully studied to determine the molecular mechanisms of organismal stress tolerance in vivo. This review will explore cell signalling modulation of stress tolerance in insects by focusing on cell signalling in a fluid transporting epithelium -the Malpighian tubule. Manipulation of specific genes and signalling pathways in only defined tubule cell types can influence the survival outcome in response to multiple environmental stressors including desiccation, immune, salt (ionic) and oxidative stress, suggesting that studies in the genetic model Drosophila melanogaster may reveal novel pathways required for stress tolerance.

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Evolved patterns and rates of water loss and ion regulation in laboratory-selected populations ofDrosophila melanogaster

Cited by 35 publications

References 44 publications

Repeated bouts of dehydration deplete nutrient reserves and reduce egg production in the mosquito Culex pipiens

Repeated bouts of dehydration deplete nutrient reserves and reduce egg production in the mosquito Culex pipiens

Insect capa neuropeptides impact desiccation and cold tolerance

Cell signalling mechanisms for insect stress tolerance

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