Evolutionary Relationships of the Triatoma matogrossensis Subcomplex, the Endemic Triatoma in Central-Western Brazil, Based on Mitochondrial DNA Sequences

Abstract

The phylogenetic relationships among species of Triatoma matogrossensis subcomplex ( T. baratai, T. guazu, T. matogrossensis, T. sordida, T. vandae, and T. williami) was addressed by using fragments of cytochrome oxidase I (COI), 16S rDNA (16S), and cytochrome b (Cytb) through Bayesian and parsimony analyses. We did not recover a monophyletic T. matogrossensis subcomplex, and their members were found clustered in three strongly supported clades, as follows: i) T. jurbergi + T. matogrossensis + T. vandae + T. garciabesi + T. sordida; ii) with T. guasayana as the sister group of clade (i); and iii) T. williami + T. guazu, however not closely related to the clade formed by the previously mentioned species. The other two endemic species from Central-Western Brazil, T. baratai and T. costalimai, were not recovered with strong clade support as related to other members of this subcomplex. Results call for a further revision in the classification of the subcomplexes within the genus Triatoma.

Mitochondrial Genes Reveal Triatoma jatai as a Sister Species to Triatoma costalimai (Reduviidae: Triatominae)

“…Gardim and others 10 and Justi and others 5 have already brought up the non-monophyly of the Matogrossensis subcomplex. Hence, T. jatai shall be considered for reevaluating this group.…”

“…4,10,14 However, Mas-Coma and Bargues 13 brought up some limitation for inferences on closely related taxa based on mitochondrial genes, particularly when dealing with sympatric or parapatric species without a clear barrier of isolation. These limitations rely mainly on introgression (sometimes followed by mitochondrial selection), complex population structure, and sex-biased gene flow.…”

“…9 Nucleotide data for COI were transformed into amino acid sequences to check the alignment for the presence of pseudogenes. 10 Combined phylogenetic analyses using both genes were run under a Bayesian framework in MrBayes (version 3.1.45). 11 We conducted two independent runs of 1 million generations using four Markov chains and sampling trees every 100 generations (discarding the first 25%).…”

Spermatogenesis and nucleolar behavior in Triatoma vandae and Triatoma williami (Hemiptera, Triatominae)

“…costalimai, T. deaneorum, T. guazu, T. jatai, T. jurbergi, T. matogrossensis, T. vandae, and T. williami (Schofield and Galvão, 2009;Obara et al, 2012;Gonçalves et al, 2013). Gardim et al (2013), in their phylogenetic reconstruction based on COI, Cytb, and 16S, did not identify the matogrossensis subcomplex as a monophyletic group, and placed its species into three different clades: 1) T. jurbergi + T. matogrossensis + T. vandae; 2) T. guasayana as the sister group of clade 1; and 3) T. williami + T. guazu. To further clarify the relationships of these species, the present study aims to describe spermatogenesis in T. vandae and the nucleolar behavior T. vandae and T. williami, with a cytotaxonomic focus.…”

Description of the diploid chromosome set of Triatoma pintodiasi (Hemiptera, Triatominae)

“…The species of the Rubrovaria subcomplex were initially grouped through morphological analyses: T. rubrovaria and T. carcavalloi categorized in the Rubrovaria subcomplex; T. circummaculata and T. limai in the Circummaculata complex; and T. klugi in the Oliverai complex (Dujardin et al, 2002). Almeida et al (2009) grouped T. circummaculata in subcomplex Rubrovaria based on phylogenetic and molecular data, and Gardim et al (2013) and Justi et al (2014) identified the subcomplex Rubrovaria as monophyletic. However, new approaches, such as experimental hybrid crosses and cytogenetic analyzes, are important and necessary to assist in understanding the evolutionary development of these vectors.…”