Evolutionary origin of a monophasic Salmonella serovar, 9,12:l,v:-, revealed by IS200 profiles and restriction fragment polymorphisms of the fljB gene

Burnens, André P.; Stanley, John R.; Sechter, I; Nicolet, Jacques

doi:10.1128/jcm.34.7.1641-1645.1996

Cited by 30 publications

(9 citation statements)

References 25 publications

(24 reference statements)

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“…The atypical Salmonella enterica 1,4, [5],12:i:-emerged a few years ago in Reunion Island and because of the close genetic relationship, it is certainly a monophasic isolate of S. Typhimurium [8]. Many studies have already explained the relationships between serovars S.1,4, [5],12:i:-and Typhimurium through the presence of IS200 by DNA microarray [25,26].…”

Section: Discussionmentioning

confidence: 99%

Epidemiological Analysis of Salmonella Enterica Serovar Typhimurium and Serovar 1,4,[5],12:i:- Isolates Determined by Pulsed Field Gel Electrophoresis and Antibiotic Susceptibility: Comparison of Isolates from Broiler Chickens, Humans and the Environment in Reunion Island

Henry¹,

Chemaly²,

Granier³

et al. 2015

TOVSJ

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Salmonella enterica ssp. enterica is a leading cause of bacterial food-borne disease outbreaks worldwide and is also an economic burden particularly in Reunion Island because its population consumes large amounts of chicken and cooks 100% chicken sausages (35 kg per capita per year). The aim of this study was to investigate the epidemiology of Salmonella Typhimurium and Salmonella 1,4,[5],12:i:-from broiler chickens, humans and the environment by using pulsed field gel electrophoresis (PFGE) and antibiotic susceptibility and to assess the significance of broiler chicken meat as a source of human infection. A total of 157 Salmonella Typhimurium and 19 S. I 4,[5],12:i:-were collected and isolated from broiler chickens, humans and the environment between October 2007 and January 2009. The PFGE of Xba1 digested chromosomal DNA gave 30 distinct profiles for Salmonella Typhimurium and S. 1,4,[5],12:i:-. Salmonella Typhimurium was characterized by a main pulsotype (B54) and accounted for 32% of all isolates. This pulsotype included isolates from many sources such as broiler chickens, poultry houses, slaughterhouses, other animal species (ducks, pigs and rodents) and humans, suggesting that it had already colonized every step of the food chain. Antibiotic susceptibility tests showed that most isolates were resistant to ampicillin, streptomycin, sulfonamides and tetracycline. The similarity of PFGE profiles of isolates from various sources and particularly from poultry and humans underlined possible transmission of Salmonella from contaminated broiler meat, but most of the isolates remained drug-sensitive. Significance and impact of study: Efforts are needed to eliminate Salmonella from poultry meat destined for human consumption. This study has also shown the importance of monitoring antimicrobial resistance in bacteria associated with animals and humans.

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Section: Discussionmentioning

confidence: 99%

Epidemiological Analysis of Salmonella Enterica Serovar Typhimurium and Serovar 1,4,[5],12:i:- Isolates Determined by Pulsed Field Gel Electrophoresis and Antibiotic Susceptibility: Comparison of Isolates from Broiler Chickens, Humans and the Environment in Reunion Island

Henry¹,

Chemaly²,

Granier³

et al. 2015

TOVSJ

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“…It could represent ancestral forms which did not acquire, though evolution, a second flagellar antigen or the required switching mechanism. Alternatively, it could originate as mutants of biphasic Salmonella, which have lost either the switching mechanism or the ability to express the second flagellar antigen (Burnens et al, 1996).…”

Section: Characterization Of Monophasic Salmonella Enterica Subsp Enmentioning

confidence: 99%

Virulence Characterization of Salmonella Typhimurium I,4,[5],12:i:-, the New Pandemic Strain

Vieira-Pinto¹,

Themudo²,

Domı́nguez³

et al. 2012

Salmonella - Distribution, Adaptation, Control Measures and Molecular Technologies

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Please use Adobe Acrobat Reader to read this book chapter for free. Just open this same document with Adobe Reader. If you do not have it, you can download it here. You can freely access the chapter at the Web Viewer here. Salmonella-Distribution, Adaptation, Control Measures and Molecular Technologies 292 dangerous clinical expression of a Salmonella infection, Typhoid fever, affects 16 million people per year, with almost 500,000 fatal cases, as estimated by the World Health Organization (Pang et al., 1998). In fact, not all Salmonella isolates identified worldwide have such devastating consequences to human or animal health. Some of the Salmonella seroptypes or serovars are strictly adapted to primates (Salmonella Typhi, Salmonella Paratyphi, Salmonella Wien) being referred to as prototrophic for man; others are strictly adapted to some animal species (Salmonella Gallinarum-Pullorum, Salmonella Abortusovis, Salmonella Abortusequi), being referred to as prototrophic to animals; however, the vast majority of the serotypes are zoonotic, being able to infect both animals and humans. Salmonella varieties differentiation is based on its antigenic mosaic, in a complex combination of somatic (O), flagelar (H) and capsular (Vi) antigens. Serotyping according to the Kauffmann-White system, established in the middle of the last century, is still recognized as the reference method for discrimination of Salmonella varieties. Each combination of different antigens found in a particular Salmonella isolate (serotype or serovar) has a specific designation, following the international nomenclature based on one hundred years of scientific contributions, which sometimes originated peculiar designations (

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“…3 kb PCR product of the Escherichia coli 16S rrnB operon was amplified and purified as described previously [11]. For IS200 typing, a 557-bp PCR product of the IS200 insertion sequence was amplified from our own Salmonella serovar Infantis isolate K1469 with the primer pair 5k-CCTAACAGGCGCATACGATC-3k and 5k-ACATCTTGCGGTCTGGCAAC-3k [13]. A 30-cycle programme (1 min at 94 xC, 0 .…”

Section: Ribotyping and Is200 Typingmentioning

confidence: 99%

Molecular characterization of Salmonella enterica subsp. enterica serovar Typhimurium DT1 isolates

Lindqvist¹,

Heinikainen²,

Siitonen

et al. 2004

Epidemiol. Infect.

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Salmonella Typhimurium DT1 is endemic to Finland and has caused human outbreaks since the 1960s. Domestic DT1 isolates (n=235) from 1972 to 1999 from human cases, animals and other sources, as well as foreign DT1 isolates from human cases (n=20) were analysed by molecular methods. Pulsed-field gel electrophoresis (PFGE) yielded 38 XbaI profiles. Of these, XbaI profile 10 was seen in 49% (125/255) of the isolates. Twelve IS200 profiles were obtained; the most common IS200 profile D was seen in 64% (33/52) of the isolates. Two clusters were formed by compilation of the XhaI-, BlnI- and SpeI-PFGE and IS200 profiles and possession of the serovar-specific virulence plasmid. The major cluster contained eight IS200 profiles, including IS200 profile D and XhaI profile 10, and had no virulence plasmid, and can be regarded as typical of the endemic Typhimurium DT1 infection.

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Evolutionary origin of a monophasic Salmonella serovar, 9,12:l,v:-, revealed by IS200 profiles and restriction fragment polymorphisms of the fljB gene

Cited by 30 publications

References 25 publications

Epidemiological Analysis of Salmonella Enterica Serovar Typhimurium and Serovar 1,4,[5],12:i:- Isolates Determined by Pulsed Field Gel Electrophoresis and Antibiotic Susceptibility: Comparison of Isolates from Broiler Chickens, Humans and the Environment in Reunion Island

Epidemiological Analysis of Salmonella Enterica Serovar Typhimurium and Serovar 1,4,[5],12:i:- Isolates Determined by Pulsed Field Gel Electrophoresis and Antibiotic Susceptibility: Comparison of Isolates from Broiler Chickens, Humans and the Environment in Reunion Island

Virulence Characterization of Salmonella Typhimurium I,4,[5],12:i:-, the New Pandemic Strain

Molecular characterization of Salmonella enterica subsp. enterica serovar Typhimurium DT1 isolates

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