Evolutionary history of H5 highly pathogenic avian influenza viruses (clade 2.3.4.4c) circulating in Taiwan during 2015–2018

Huang, Chih-Wei; Chen, Li-Hsuan; Lee, Donghun; Liu, Yu-Pin; Li, Wan-Chen; Lee, Ming-Shiuh; Chen, Yen-Ping; Lee, Fan; Chiou, Chwei-Jang; Lin, Yu‐Feng

doi:10.1016/j.meegid.2021.104885

Cited by 8 publications

(12 citation statements)

References 48 publications

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“…The highly pathogenic avian influenza (HPAI) virus A/goose/Guangdong/1/96 H5N1 (Gs/GD) lineage caused global epidemics not only in migratory birds and domestic poultry but also in the human population. Taiwan had previously encountered an outbreak of HPAI clade 2.3.4.4 derived from Gs/GD lineage in poultry [ 3 ]. Clade 2.3.4.4 has evolved since 2008 and had several reassortant neuraminidase (NA) genes with N2, N3, N5, N6, and N8 variants.…”

Section: Introductionmentioning

confidence: 99%

“…Phylogenetic analysis based on the sequence of hemagglutinin (HA) and M gene reveals that HPAI H5Nx viruses in October 2014 were related to two HPAI H5N8 viruses identified from wild birds in Japan. Two subsequent outbreaks in late 2014 and 2016 resulted from seven H5Nx reassortant genes that spread through Taiwan and eight novel introduced internal genes mostly derived from the gene pool of avian influenza virus circulating in wild birds in Asia [ 3 ]. In several outbreaks in past decades, it has been reported that the sources of infection were novel HPAI clade 2.3.4.4 viruses.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of Avian Influenza H5N6 Virus-like Particles as a Broad-Spectrum Vaccine Candidate against H5Nx Viruses

Tai

Cheng

Wang

et al. 2022

Viruses

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Highly pathogenic avian influenza (HPAI) clade 2.3.4.4 viruses have been reported to be the source of infections in several outbreaks in the past decades. In a previous study, we screened out a broad-spectrum virus strain, H5N6-Sichuan subtype, by using a lentiviral pseudovirus system. In this project, we aimed to investigate the potential of H5N6 virus-like particles (VLPs) serving as a broad-spectrum vaccine candidate against H5Nx viruses. We cloned the full-length M1 gene and H5, N6 genes derived from the H5N6-Sichuan into pFASTBac vector and generated the VLPs using the baculovirus-insect cell system. H5N6 VLPs were purified by sucrose gradient centrifugation, and the presence of H5, N6 and M1 proteins was verified by Western blot and SDS-PAGE. The hemagglutination titer of H5N6 VLPs after purification reached 5120 and the particle structure remained as viewed by electron microscopy. The H5N6 VLPs and 293T mammalian cell-expressed H5+N6 proteins were sent for mice immunization. Antisera against the H5+N6 protein showed 80 to 320 neutralizing antibody titers to various H5Nx pseudoviruses. In contrast, H5N6 VLPs not only elicited higher neutralizing antibody titers, ranging from 640 to 1280, but also induced higher IL-2, IL-4, IL-5, IFN-γ and TNF production, thus indicating that H5N6 VLPs may be a potential vaccine candidate for broad-spectrum H5Nx avian influenza vaccines.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Investigation of Avian Influenza H5N6 Virus-like Particles as a Broad-Spectrum Vaccine Candidate against H5Nx Viruses

Tai

Cheng

Wang

et al. 2022

Viruses

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“… 52 , 53 , 54 , 55 Full genome sequencing provides further opportunities for phylodynamic modeling, that explores the joint dynamics of epidemiological and evolutionary processes to determine the origin and spread of human, animal, and plant pathogens. 56 , 57 , 58 Phylodynamic modeling has made significant progress in recent years due to increasingly available genomic data and advances in statistical modeling but there remain many challenges to the widespread application of phylodynamic models for biosecurity. These challenges include accounting for evolutionary complexities such as changing mutation rates, selection, reassortment, and recombination, as well as epidemiological complexities such as stochastic population dynamics, and host population structure.…”

Section: Emerging Advances In Biosecuritymentioning

confidence: 99%

Emerging advances in biosecurity to underpin human, animal, plant, and ecosystem health

Hulme¹,

Beggs²,

Binny³

et al. 2023

iScience

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“…Following migratory bird flyways, the virus spread to Japan, the USA and Taiwan by the end of that year [18][19][20] . In 2015, Taiwan experienced a devastating epidemic in domestic birds and culled over 5 million birds to curb localised outbreaks 21 . This marked the first significant transmission of HPAI GsGd viruses in the country 22,23 .…”

Section: Introductionmentioning

confidence: 99%

From emergence to endemicity: highly pathogenic H5 avian influenza viruses in Taiwan

Li,

Ko,

Hughes

et al. 2024

Preprint

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A/goose/Guangdong/1/96-like (GsGd) highly pathogenic avian influenza (HPAI) H5 viruses cause severe outbreaks in poultry when introduced. Since emergence in 1996, control measures in most countries have suppressed local GsGd transmission following introductions, making persistent transmission in domestic birds rare. However, geographical expansion of clade 2.3.4.4 sublineages has raised concern about establishment of endemic circulation, while mechanistic drivers leading to endemicity remain unknown. We reconstructed the evolutionary history of GsGd sublineage, clade 2.3.4.4c, in Taiwan using a time-heterogeneous rate phylogeographic model. During Taiwan’s initial epidemic wave (January 2015 - August 2016), we inferred that localised outbreaks had multiple origins from rapid spread between counties/cities nationwide. Subsequently, outbreaks predominantly originated from a single county, Yunlin, where persistent transmission harbours the trunk viruses of the sublineage. Endemic hotspots determined by phylogeographic reconstruction largely predicted the locations of re-emerging outbreaks in Yunlin. The transition to endemicity involved a shift to chicken-dominant circulation, following the initial bidirectional spread between chicken and domestic waterfowl. Our results suggest that following their emergence in Taiwan, source-sink dynamics from a single county have maintained GsGd endemicity, pointing to where control efforts should be targeted to eliminate the disease.

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Evolutionary history of H5 highly pathogenic avian influenza viruses (clade 2.3.4.4c) circulating in Taiwan during 2015–2018

Cited by 8 publications

References 48 publications

Investigation of Avian Influenza H5N6 Virus-like Particles as a Broad-Spectrum Vaccine Candidate against H5Nx Viruses

Investigation of Avian Influenza H5N6 Virus-like Particles as a Broad-Spectrum Vaccine Candidate against H5Nx Viruses

Emerging advances in biosecurity to underpin human, animal, plant, and ecosystem health

From emergence to endemicity: highly pathogenic H5 avian influenza viruses in Taiwan

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