Evolutionary “hide and seek” between bacterial flagellin and the plant immune system

Stringlis, Ioannis A.; Pieterse, Corné M. J.

doi:10.1016/j.chom.2021.03.010

Cited by 11 publications

(8 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of particular interest is the gene FLAGELLIN-SENSITIVE 2 ( FLS2 ) overlapping with 39 top SNPs in our study (Data Set 11). FLS2 encodes for a leucine-rich repeat receptor-like kinase first identified in the perception of the bacterial elicitor flagellin (Gómez-Gómez and Boller 2000) and then confirmed as key in microbe-associated molecular patterns (MAMP)-triggered immunity (MTI) (Stringlis and Pieterse 2021). Although FLS2 detects flagellin from both pathogenic and beneficial bacteria by the 22-amino-acid N-terminal epitope flg22 (Stringlis et al 2018), FLS2 was never proposed as a candidate gene in GWAS of response to bacterial pathogens (https://aragwas.1001genomes.org/#/gene/AT5G46330), despite the identification of more than 100 amino acid changes in FLS2 among >1,000 worldwide accessions (Bai et al 2022).…”

Section: Resultsmentioning

confidence: 99%

The genetic architecture of adaptation to leaf and root bacterial microbiota inArabidopsis thaliana

Roux

Frachon

Bartoli

2022

Preprint

View full text Add to dashboard Cite

Understanding the role of host genome in modulating microbiota variation is a need to shed light into the holobiont theory and overcome the current limits on the description of host-microbiota interactions at the genomic and molecular levels. However, the host genetic architecture structuring microbiota is only partly described in plants. In addition, most association genetic studies on microbiota are often carried out outside the native habitats where the host evolve and the identification of signatures of local adaptation on the candidate genes has been overlooked. To fill these gaps and dissect the genetic architecture driving adaptive plant-microbiota interactions, we adopted a Genome-Environmental-Association (GEA) analysis on 141 whole-genome sequenced natural populations of Arabidopsis thaliana characterized in situ for their leaf and root bacterial communities and a large range of environmental descriptors (i.e. climate, soil and plant communities). Across 194 microbiota traits, a much higher fraction of among-population variance was explained by the host genetics than by ecology, with the plant neighborhood as the main ecological driver of microbiota variation. Importantly, the relative importance of host genetics and ecology expressed a phylogenetic signal at the family and genus level. In addition, the polygenic architecture of adaptation to bacterial communities was highly flexible between plant compartments and seasons. Relatedly, signatures of local adaptation were stronger on QTLs of the root microbiota in spring. Finally, we provide evidence that plant immunity, in particular the FLS2 gene, is a major source of adaptive genetic variation structuring bacterial assemblages in A. thaliana.

show abstract

Section: Resultsmentioning

confidence: 99%

The genetic architecture of adaptation to leaf and root bacterial microbiota inArabidopsis thaliana

Roux

Frachon

Bartoli

2022

Preprint

View full text Add to dashboard Cite

show abstract

“…In plants, FLS2 appears to be particularly vulnerable to evolutionary selection pressures. More than 100 amino acid changes in FLS2 have been identified using genome mining in >1,000 Arabidopsis accessions, indicating that FLS2 sequence variants seem to have evolved an ability to detect various flg22 variants to ensure proper immune activation (Parys et al, 2021; Stringlis and Pieterse, 2021). The diversified variants of flg22 evade the recognition of FLS2, while receptor FLS2 recognizes more flg22 variants through variation in amino acid, which shows the co‐evolution between plants and microbes.…”

Section: Main Factors Affecting the Root Microbiotamentioning

confidence: 99%

The root microbiome: Community assembly and its contributions to plant fitness

Bai

Liu

Qiu

et al. 2022

JIPB

163

View full text Add to dashboard Cite

show abstract

“…The relationship between pathogens and hosts is not unlike an 'arms race' that reciprocally drives the immune system (mentioned above) and resistance to escalate because of the wide adaptability and heightened virulence of plant pathogens (Stringlis and Pieterse, 2021;Wang et al, 2021). However, plants could recruit some mutualistic microbes as partners to enhance their own immunity and to suppress pathogen infection.…”

Section: Acquisition Of Plant Stress Resistance From the Plant-symbiont-pathogen Relationshipmentioning

confidence: 99%

Transcriptomic and Metabolomic Approaches Deepen Our Knowledge of Plant–Endophyte Interactions

et al. 2022

View full text Add to dashboard Cite

In natural systems, plant–symbiont–pathogen interactions play important roles in mitigating abiotic and biotic stresses in plants. Symbionts have their own special recognition ways, but they may share some similar characteristics with pathogens based on studies of model microbes and plants. Multi-omics technologies could be applied to study plant–microbe interactions, especially plant–endophyte interactions. Endophytes are naturally occurring microbes that inhabit plants, but do not cause apparent symptoms in them, and arise as an advantageous source of novel metabolites, agriculturally important promoters, and stress resisters in their host plants. Although biochemical, physiological, and molecular investigations have demonstrated that endophytes confer benefits to their hosts, especially in terms of promoting plant growth, increasing metabolic capabilities, and enhancing stress resistance, plant–endophyte interactions consist of complex mechanisms between the two symbionts. Further knowledge of these mechanisms may be gained by adopting a multi-omics approach. The involved interaction, which can range from colonization to protection against adverse conditions, has been investigated by transcriptomics and metabolomics. This review aims to provide effective means and ways of applying multi-omics studies to solve the current problems in the characterization of plant–microbe interactions, involving recognition and colonization. The obtained results should be useful for identifying the key determinants in such interactions and would also provide a timely theoretical and material basis for the study of interaction mechanisms and their applications.

show abstract

Evolutionary “hide and seek” between bacterial flagellin and the plant immune system

Cited by 11 publications

References 11 publications

The genetic architecture of adaptation to leaf and root bacterial microbiota inArabidopsis thaliana

The genetic architecture of adaptation to leaf and root bacterial microbiota inArabidopsis thaliana

The root microbiome: Community assembly and its contributions to plant fitness

Transcriptomic and Metabolomic Approaches Deepen Our Knowledge of Plant–Endophyte Interactions

Contact Info

Product

Resources

About