Evolutionary genomics analysis reveals gene expansion and functional diversity of arylalkylamine <i>N</i>‐acetyltransferases in the Culicinae subfamily of mosquitoes

Tang, Yu; Chen, Huaqing; Lin, Zhengzhong; Zhang, Lei; Upadhyay, Archana; Liao, Chenghong; Merkler, David J.; Han, Qian

doi:10.1111/1744-7917.13100

Cited by 5 publications

(2 citation statements)

References 53 publications

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“…AANAT appears to be subject to specific selection pressures in different groups. For example, the functional diversification of the insect AANAT family has been shaped by positive selection [46]. The whole-gene replication process of AANAT in bony fishes may be attributed to its rapid evolution that facilitates genetic changes in response to environmental stress [47].…”

Section: Discussionmentioning

confidence: 99%

Adaptive Evolution of the Greater Horseshoe Bat AANAT: Insights into the Link between AANAT and Hibernation Rhythms

Zhao,

Wang,

Liu

et al. 2024

Animals

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Arylalkylamine N-acetyltransferase (AANAT) is a crucial rate-limiting enzyme in the synthesis of melatonin. AANAT has been confirmed to be independently duplicated and inactivated in different animal taxa in order to adapt to the environment. However, the evolutionary forces associated with having a single copy of AANAT remain unclear. The greater horseshoe bat has a single copy of AANAT but exhibits different hibernation rhythms in various populations. We analyzed the adaptive evolution at the gene and protein levels of AANAT from three distinct genetic lineages in China: northeast (NE), central east (CE), and southwest (SW). The results revealed greater genetic diversity in the AANAT loci of the NE and CE lineage populations that have longer hibernation times, and there were two positive selection loci. The catalytic capacity of AANAT in the Liaoning population that underwent positive selection was significantly higher than that of the Yunnan population (p < 0.05). This difference may be related to the lower proportion of α helix and the variation in two interface residues. The adaptive evolution of AANAT was significantly correlated with climate and environment (p < 0.05). After controlling for geographical factors (latitude and altitude), the evolution of AANAT by the negative temperature factor was represented by the monthly mean temperature (r = −0.6, p < 0.05). The results identified the gene level variation, functional adaptation, and evolutionary driving factors of AANAT, provide an important foundation for further understanding the adaptive evolution of the single copy of AANAT in pteropods, and may offer evidence for adaptive hibernation rhythms in bats.

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Section: Discussionmentioning

confidence: 99%

Adaptive Evolution of the Greater Horseshoe Bat AANAT: Insights into the Link between AANAT and Hibernation Rhythms

Zhao,

Wang,

Liu

et al. 2024

Animals

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“…GNAT proteins acetylate different substrates and play a role in bacterial antibiotic resistance (Czub et al, 2018). Arylalkylamine N-acetyltransferase (aaNAT) belonging to the GNAT family has received attention due to its involvement in regulating the synthesis of neuromediators in insects (Tang et al, 2022). Hence, it could be hypothesized S. marcescens pathogenesis in insects may well be carried out via neurotoxicity.…”

Section: Discussionmentioning

confidence: 99%

The man, the plant, and the insect: shooting host specificity determinants in Serratia marcescens pangenome

Shikov,

Merkushova,

Savina

et al. 2023

Front. Microbiol.

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IntroductionSerratia marcescens is most commonly known as an opportunistic pathogen causing nosocomial infections. It, however, was shown to infect a wide range of hosts apart from vertebrates such as insects or plants as well, being either pathogenic or growth-promoting for the latter. Despite being extensively studied in terms of virulence mechanisms during human infections, there has been little evidence of which factors determine S. marcescens host specificity. On that account, we analyzed S. marcescens pangenome to reveal possible specificity factors.MethodsWe selected 73 high-quality genome assemblies of complete level and reconstructed the respective pangenome and reference phylogeny based on core genes alignment. To find an optimal pipeline, we tested current pangenomic tools and obtained several phylogenetic inferences. The pangenome was rich in its accessory component and was considered open according to the Heaps’ law. We then applied the pangenome-wide associating method (pan-GWAS) and predicted positively associated gene clusters attributed to three host groups, namely, humans, insects, and plants.ResultsAccording to the results, significant factors relating to human infections included transcriptional regulators, lipoproteins, ABC transporters, and membrane proteins. Host preference toward insects, in its turn, was associated with diverse enzymes, such as hydrolases, isochorismatase, and N-acetyltransferase with the latter possibly exerting a neurotoxic effect. Finally, plant infection may be conducted through type VI secretion systems and modulation of plant cell wall synthesis. Interestingly, factors associated with plants also included putative growth-promoting proteins like enzymes performing xenobiotic degradation and releasing ammonium irons. We also identified overrepresented functional annotations within the sets of specificity factors and found that their functional characteristics fell into separate clusters, thus, implying that host adaptation is represented by diverse functional pathways. Finally, we found that mobile genetic elements bore specificity determinants. In particular, prophages were mainly associated with factors related to humans, while genetic islands-with insects and plants, respectively.DiscussionIn summary, functional enrichments coupled with pangenomic inferences allowed us to hypothesize that the respective host preference is carried out through distinct molecular mechanisms of virulence. To the best of our knowledge, the presented research is the first to identify specific genomic features of S. marcescens assemblies isolated from different hosts at the pangenomic level.

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Function, structure, evolution, regulation of a potent drug target, arylalkylamine N-acetyltransferase

Zhang¹,

Tang²,

Merkler³

et al. 2023

Regulation of Downstream Targets

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Evolutionary genomics analysis reveals gene expansion and functional diversity of arylalkylamine N‐acetyltransferases in the Culicinae subfamily of mosquitoes

Cited by 5 publications

References 53 publications

Adaptive Evolution of the Greater Horseshoe Bat AANAT: Insights into the Link between AANAT and Hibernation Rhythms

Adaptive Evolution of the Greater Horseshoe Bat AANAT: Insights into the Link between AANAT and Hibernation Rhythms

The man, the plant, and the insect: shooting host specificity determinants in Serratia marcescens pangenome

Function, structure, evolution, regulation of a potent drug target, arylalkylamine N-acetyltransferase

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