Evolutionary Genetics of Microbial Symbiosis

Baldo, Laura; Werren, John H.

doi:10.3390/genes12030327

Cited by 4 publications

(1 citation statement)

References 11 publications

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“…2016 , Radhakrishnan et al. 2020 , Baldo and Werren 2021 ). These signaling pathways comprise a well-conserved network known as the ‘common symbiosis signalling pathway’ (CSSP) ( Oldroyd 2013 , Radhakrishnan et al.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Proteomics at Early Stages of the Symbiotic Interaction Between Oryza sativa and Nostoc punctiforme Reveals Novel Proteins Involved in the Symbiotic Crosstalk

Álvarez

Brenes‐Álvarez

Molina-Heredia

et al. 2022

Plant and Cell Physiology

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Symbiosis between cyanobacteria and plants is considered pivotal for biological nitrogen deposition in terrestrial ecosystems. Despite extensive knowledge of the ecology of plant-cyanobacterium symbioses, little is known about the molecular mechanisms involved in recognition between partners. Here, we conducted a quantitative sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS) pipeline to analyse protein changes in Oryza sativa and Nostoc punctiforme during early events of symbiosis. We found differentially expressed proteins in both organisms linked to several biological functions, including signal transduction, adhesion, defence-related proteins, and cell wall modification. In Nostoc punctiforme we found increased expression in 62 proteins that have been previously described in other Nostoc-plant symbioses, reinforcing the robustness of our study. Our findings reveal new proteins activated in the early stages of the Nostoc-Oryza symbiosis that might be important for the recognition between the plant and the host. Oryza mutants in genes in the common symbiosis signalling pathway (CSSP) show a reduced colonization efficiency providing first insights on the involvement of the CSSP for the accommodation of N. punctiforme inside the plant cells. This information may have long-term implications for a greater understanding of the symbiotic interaction between Nostoc and land plants.

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Section: Introductionmentioning

confidence: 99%

Quantitative Proteomics at Early Stages of the Symbiotic Interaction Between Oryza sativa and Nostoc punctiforme Reveals Novel Proteins Involved in the Symbiotic Crosstalk

Álvarez

Brenes‐Álvarez

Molina-Heredia

et al. 2022

Plant and Cell Physiology

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Characterizing the microbiome composition of the nares, oropharynx, and hand of monozygotic twins and its interplay with Staphylococcus aureus carriage

Dalman,

Simison,

Nielson

et al. 2023

Preprint

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Background Staphylococcus aureus is a gram-positive bacterium commonly found in the nares and oropharynx of one in three individuals and has the potential to cause significant health problems. With antibiotic resistant strains causing 11,000 deaths yearly and ~ 2% of the population nasally colonized with MRSA, a search for predictive markers and causal relationships between carriage have been long-sought goals. Within our study, we leveraged monozygotic twin participants in concert with spatial microbiome analyses to characterize impacts of S. aureus on composition. Results: We recruited 147 monozygotic twin pairs and characterized three sites: nares, oropharynx, and hand microbiomes using 16S rRNA v3-v4 sequencing in addition to S. aureus carriage status. Prevalence of S. aureus was highest in the oropharynx followed by nares and hand with concordance between twin pairs highest in the nares, followed by oropharynx. All microbiome sites were significantly modified by the presence of S. aureus as illustrated by beta diversity and DESeq2 analyses. Microbiome composition was most similar in twins’ nares that were S. aureus culture positive concordant, whereas twins that were culture negative concordant had the most similarity in the Oropharynx. Of significance, Moraxella nonliquefacians and Capnocytophaga were inversely associated with S. aureus in the nares and oropharynx, respectively. Based on Maaslin2 analyses, we identify that S. aureus prevalence significantly affected pathways associated with Biosynthesis of Secondary Metabolites, Infectious Diseases, Amino Acid Metabolism, Cellular Processes and Signaling, and Immune System Diseases only in the nares microbiome. Conclusions: This improved understanding of S. aureus colonization on nares, oropharynx, and hand microbiomes in monozygotic twin pairs is a further step towards unraveling the degree to which the microbiome are influenced by host genetics and S. aureus carriage.

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Of Cockroaches and Symbionts: Recent Advances in the Characterization of the Relationship between Blattella germanica and Its Dual Symbiotic System

Latorre

Domínguez-Santos

García-Ferris

et al. 2022

Life

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Mutualistic stable symbioses are widespread in all groups of eukaryotes, especially in insects, where symbionts have played an essential role in their evolution. Many insects live in obligate relationship with different ecto- and endosymbiotic bacteria, which are needed to maintain their hosts’ fitness in their natural environment, to the point of even relying on them for survival. The case of cockroaches (Blattodea) is paradigmatic, as both symbiotic systems coexist in the same organism in two separated compartments: an intracellular endosymbiont (Blattabacterium) inside bacteriocytes located in the fat body, and a rich and complex microbiota in the hindgut. The German cockroach Blattella germanica is a good model for the study of symbiotic interactions, as it can be maintained in the laboratory in controlled populations, allowing the perturbations of the two symbiotic systems in order to study the communication and integration of the tripartite organization of the host–endosymbiont–microbiota, and to evaluate the role of symbiotic antimicrobial peptides (AMPs) in host control over their symbionts. The importance of cockroaches as reservoirs and transmission vectors of antibiotic resistance sequences, and their putative interest to search for AMPs to deal with the problem, is also discussed.

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Evolutionary Genetics of Microbial Symbiosis

Abstract: Symbiosis is the living together of dissimilar organisms [...]

Cited by 4 publications

References 11 publications

Quantitative Proteomics at Early Stages of the Symbiotic Interaction Between Oryza sativa and Nostoc punctiforme Reveals Novel Proteins Involved in the Symbiotic Crosstalk

Quantitative Proteomics at Early Stages of the Symbiotic Interaction Between Oryza sativa and Nostoc punctiforme Reveals Novel Proteins Involved in the Symbiotic Crosstalk

Characterizing the microbiome composition of the nares, oropharynx, and hand of monozygotic twins and its interplay with Staphylococcus aureus carriage

Of Cockroaches and Symbionts: Recent Advances in the Characterization of the Relationship between Blattella germanica and Its Dual Symbiotic System

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