Evolutionary Genetic Analysis of the cytochrome b gene variation in the Salmo trutta fario with other salmons

Abstract

PCR amplification and direct sequencing of regions of the cytochrome b mitochondrial genes was carried out on Iranian populations of the salmo trutta fario species. Samples of salmo trutta fario were also examined and used as an out group in the phylogenetic analysis. Results based on 1192 bp indicated differentiation between fario morphs from Iran and other salmonids also Iran and other country had collected. Despite the large phenotypic differences within salmonids, very low genetic variation was found. On the basis of the cytochrome b sequences studied, Salmo carpio and Salmo fibreni, which have been described as good species, there were high homology between salmo trutta fario , salmo trutta and salmo trutta caspius (99%), but regards salmo salar the rate of homology 93%. Regards salmo trutta fario, in hatchery trout has not red colour strips time of hatchery in salmo trutta fario whereas has a bluish grey body colour, However, they are larger than Atlantic salmon, salmo trutta caspius and salmo trutta, more regular in shape, and less intensively pigmented, moreover there are red spots are always observed in populations, however, the shape and size of salmo trutta fario is different with salmo trutta caspius and salmo trutta but the among of homology were high (99%). The salmo trutta fario and salmo trutta caspius are living in the Rivers of North of Iran that connected to Caspian Sea; perhaps these species with themselves has been conjugated, and regards salmo salar is living in the Atlantic Ocean and other Sea and Rivers connected to it, that so far to salmo trutta fario populations in Iran. However we cannot report exactly that how much homology between sequences, because already there are not complete sequence in GeneBank regards salmo trutta caspius and salmo trutta fario.

The relation between growth hormone (GH) gene and Cytochrome b gene in three salmon types

“…(Accession number, JN241634.1), containing, five exons and six introns in the length. (Rezaei and Akhshabi, 2011 a and b;Rezaei and Akhshabi, 2012). The result of sequence in Salmo Salar (Johansen et al, 1989), however there were not report regards sequence of GH gene in S. t. fario, but also we can conclude the variation is low between S. t. fario , S. Salar and S. t. Caspius, because the result of mitochondrial DNA that related to maternal traits had been high homology between salmonids.…”

Comparison of phylogenetic analysis in the natural salmonids by using growth hormone (GH) gene

“…GH gene in bovine changed a single nucleotide polymorphism in fifth exon (Lucy et al, 1991;Zhang et al, 1992;Yao et al, 1996). The many genes were used for polymorphism of salmonids, mitochondrial DNA has been studied extensively in numerous fish species by restriction site analysis of the entire mitochondrial genome or by 'micro restriction' mapping and direct sequencing of the selectively amplified genes (Beckenbach, 1991;Bernatchez et al, 1992;Whitmore et al, 1992;Ovenden et al, 1993), that used some genes for this means, including, cytochrome b gene in salmo treutta fario (Rezaei and Akhshabi, 2012), cytochrome b in salmo trutta caspius (Jamshidi and Kalbasi, 2009), and other genes in mitochondrial DNA. The mitochondrial DNA more inherited maternal traits than paternal traits,but in GH gene more inherited paternal trait on the nuclear DNA genomics.…”