Evolutionary, epidemiological, demographical, and geographical dissection of porcine bocavirus in China and America

Zhang, Qiangzhe; Zhang, Caihong; Gao, Ming; He, Xiping; Diao, Yinghua; Goyal, Sagar M.; Mor, Sunil K.; Huang, Jinhai

doi:10.1016/j.virusres.2014.09.012

Cited by 14 publications

(16 citation statements)

References 28 publications

(29 reference statements)

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“…This is similar to previous studies which reported that 12.59% (50/397) (Cheng et al, 2010), 11.4% (46/403) (Zhang et al, 2015a) and 16.5% (55/333) (Lau et al, 2011) of Chinese pigs samples were positive. Among the genogroups, PBoV G1, G2 and G3 had been reported to have varying prevalence rates ranging from 1.5 to 88% for G1, 4.8 to 64.4% for G2 and 8.7 to 81.3% for G3 in pig herds from different countries including China (Jiang et al, 2014;Xiao, Halbur, and Opriessnig, 2013;Zhang et al, 2015a). In the current study, the detection rate of PBoV G1, G2 and G3 was 1.3%, 2.6% and 12.3%, respectively.…”

Section: Discussionsupporting

confidence: 92%

Development and validation of a multiplex conventional PCR assay for simultaneous detection and grouping of porcine bocaviruses

Zheng

Liu

Opriessnig

et al. 2016

Journal of Virological Methods

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Porcine bocavirus (PBoV), a newly described porcine parvovirus, has received attention because it can be commonly identified in clinically affected pigs including pigs with post-weaning multisystemic wasting syndrome (PWMS) and pigs with diarrhea. In recent years, novel PBoVs have been identified and were classified into three genogroups, but the ability to detect and classify these novel PBoVs is not comprehensive to date. In this study, a multiplex conventional PCR assay for simultaneous detection and grouping of PBoVs was developed by screening combinations of mixed primer pairs followed by optimization of the PCR conditions. This method exclusively amplifies targeted fragments of 531bp from the VP1 gene of PBoV G1, 291bp from the NP1 gene of PBoV G2, and 384bp from the NP1/VP1 gene of PBoV G3. The assay has a detection limit of 1.0×10(3)copies/μL for PBoV G1 4.5×10(3) for PBoV G2 and 3.8×10(3) for PBoV G3 based on testing mixed purified plasmid constructs containing the specific viral target fragments. The performance of the multiplex PCR assay was comparable to that of the single PCRs which used the same primer pairs. Using the newly established multiplex PCR assay, 227 field samples including faeces, serum and tissue samples from pigs were investigated. All three PBoV genogroups were detected in the clinical samples with a detection rate of 1.3%, 2.6% and 12.3%, respectively for PBoV G1, G2 and G3. Additionally, coinfections with two or more PBoV were detected in 1.7% of the samples investigated. These results indicate the multiplex PCR assay is specific, sensitive and rapid, and can be used for the detection and differentiation of single and multiple infections of the three PBoV genogroups in pigs.

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Section: Discussionsupporting

confidence: 92%

Development and validation of a multiplex conventional PCR assay for simultaneous detection and grouping of porcine bocaviruses

Zheng

Liu

Opriessnig

et al. 2016

Journal of Virological Methods

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“…According to the qPCR detection results of 281 clinical samples, 148 samples (148/281, 52.67%) were positive for PBoV1/2, 117 samples (117/281, 41.63%) were positive for PBoV3/4/5, 55 samples (55/281, 19.57%) were positive for both PBoV1/2 and PBoV3/4/5, and PBoVs were detected on 32 pig farms in 17 cities except Pingdingshan (Table 1), with 86.89% (32/37) of pig farms harboring PBoVs, indicating that PBoV was currently circulating in swine herds in central China. Overall, the prevalence of PBoV was 74.73% in central China (210/281), and it was much higher than that of previous reports (Zhang et al 2015;Zhou et al 2018b) which showed that PBoV mainly distributed in the east and south coastal areas of China. The results suggested that PBoV has broadly distributed among swine farms in diarrheal piglets in China.…”

Section: Resultscontrasting

confidence: 64%

“…To date, PBoVs have been reported in pigs in 20 provinces or regions in China with the prevalence between 7.3% and 64% (Wang et al, 2014;Zhang et al, 2015;Zhou et al, 2018). However, little is known about the presence of PBoV in central China.…”

Section: Introductionmentioning

confidence: 99%

Detection and genetic characteristic of porcine bocavirus in central China

Zheng¹,

Cui²,

Han³

et al. 2020

Preprint

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To investigate the epidemic profile and genetic diversity of porcine bocavirus (PBoV), 281 clinical samples including 236 intestinal tissue samples and 45 fecal samples were collected from diarrheal piglets in 37 different pig farms of central China, and two SYBR Green I-based quantitative PCR assays were developed to detect PBoV1/2 and PBoV3/4/5 respectively. The results showed the detection limits of two assays were 1.66 × 101 genome copies/μl of PBoV1/2 and 3.3 × 101 copies/μL of PBoV 3/4/5. 148 (52.67%) of the 281 clinical samples were positive for PBoV1/2, 117 (41.63%) were positive for PBoV3/4/5, 55 (19.57%) were positive for both PBoV1/2 and PBoV3/4/5, and 86.49% (32/37) of the pig farms were positive for PBoV. Subsequently, complete genomic sequences of two PBoV strains (designated CH/HNZM and PBoV-TY) from two different farms were sequenced. The phylogenetic analysis demonstrated that the two PBoV strains obtained in this study belonged to the PBoV2 group and had a close relationship with other 12 PBoV2 strains, but differed genetically from PBoV1, PBoV3/4/5 and 7 other bocaviruses. CH/HNZM and PBoV-TY were closely related to the PBoV strain GD18 (KJ755666) which may be derived from PBoV strains 0912/2012 (MH558677) and 57AT-HU (KF206160) through the recombination analysis. Compared with reference strain ZJD (HM053694)-China, a higher amino acid variation was found in the NS1 protein of CH/HNZM and PBoV-TY. These results extend our understanding of the molecular epidemiology and evolution of PBoV.

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“…Previous molecular epidemiological studies showed that pigs had different PBoV infection rate and different geographical distribution of the PBoV subgroups (Zhai et al, 2010;Jiang et al, 2014;Zhang et al, 2015a). Our studies showed that PBoV infection was also present in the piglets in Xinjiang China with an overall infection rate of 5.77%.…”

Section: Discussionsupporting

confidence: 47%

Molecular detection and genetic diversity of porcine bocavirus in piglets in China

Meng¹,

Qiao²,

Gong³

et al. 2018

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Porcine bocavirus (PBoV) is a recently discovered, non-enveloped and single-stranded DNA virus that can infect pigs. In order to understand PBoV infection and its genetic characterization in piglets in Xinjiang China, PBoV was detected by PCR in 156 clinical samples from 1-month-old piglets. PBoV was detected in 9 clinical samples, with a prevalence rate of 5.77% (9/156). Then nonstructural protein NS1 gene was amplified, sequenced and used for phylogenetic analysis. The results showed that the prevalence rate in the sick piglets was 9.33% (7/75), which is significantly higher than that in the healthy piglets (2.47%, 2/81). The nucleotide sequences of NS1 gene share high identities (96.1-99.2%) within the same groups of PBoVs. Phylogenetic analysis based on complete nucleotide sequence of NS1 gene showed that PBoV strains can be classified into three genetic groups, among which group I contains PBoV1 strains, group II contains PBoV2 strains, and group III contains PBoV3, PBoV4 and PBoV5 strains. Porcine/XJ-12, porcine/XJ-27, porcine/XJ-65, and porcine/XJ-145 had close genetic distance with subgroup 1, belonging to group I; strains porcine/XJ-79 and porcine/XJ-134 were clustered with subgroup 2, belonging to group II, while porcine/XJ-8, porcine/XJ-52 and porcine/XJ-96 were clustered with subgroup 3, which belonged to group III. This study demonstrated for the first time that PBoV strains in Xinjiang belong to three subgroups of three different genetic groups, indicating a substantial genetic diversity of the epidemic strains circulating in China, which provided the useful epidemiological data for scientific control and prevention of this disease in farm pigs.

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Evolutionary, epidemiological, demographical, and geographical dissection of porcine bocavirus in China and America

Cited by 14 publications

References 28 publications

Development and validation of a multiplex conventional PCR assay for simultaneous detection and grouping of porcine bocaviruses

Development and validation of a multiplex conventional PCR assay for simultaneous detection and grouping of porcine bocaviruses

Detection and genetic characteristic of porcine bocavirus in central China

Molecular detection and genetic diversity of porcine bocavirus in piglets in China

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