Evolutionary Dynamics of rDNAs and U2 Small Nuclear DNAs in Triportheus (Characiformes, Triportheidae): High Variability and Particular Syntenic Organization

Yano, Cássia Fernanda; Bertollo, Luiz Antônio Carlos; Rebordinos, Laureana; Merlo, Manuel Alejandro; Liehr, Thomas; Portela-Bens, Silvia; Cioffi, Marcelo de Bello

doi:10.1089/zeb.2016.1351

Cited by 35 publications

(30 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The synteny of 5S rRNA and U2 snRNA has been observed in other eukaryotes [Cross and Rebordinos, 2005;Manchado et al, 2006;Scacchetti et al, 2015;Yano et al, 2017]. The localization of the 5S rRNA, U2 snRNA, and histone gene families in the same chromosome arm in our study is in agreement with the trend of clustering of housekeeping genes, genes that are highly expressed and required for basal cellular function, proposed for the human genome [Lercher et al, 2002].…”

Section: Discussionsupporting

confidence: 91%

Chromosomal Mapping of Repeat DNA in Bergiaria westermanni (Pimelodidae, Siluriformes): Localization of 45S rDNA in B Chromosomes

Malimpensa

Traldi²,

Toyama³

et al. 2018

Cytogenet Genome Res

View full text Add to dashboard Cite

The occurrence of repetitive DNA in autosomes and B chromosomes of Bergiaria westermanni was examined using conventional and molecular cytogenetic techniques. This species exhibited 2n = 56 chromosomes, with intra- and interindividual variation in the number of heterochromatic B chromosomes (from 0 to 4). The 5S rDNA was localized in pairs 1 and 5, and histone probes (H1, H3, and H4) and U2 small nuclear RNA were syntenic with 5S rDNA in pair 5. Histone sequences were also located in chromosome pair 14. The (GATA)_n sequence was dispersed throughout the autosomes and B chromosomes, with clusters (microsatellite accumulation) in some chromosome regions. The telomeric probe revealed no signs of chromosomal rearrangements in the genome of B. westermanni. The 45S rDNA sites were detected in the terminal region of pair 27; these sites corresponded to a GC-rich heterochromatin block. In addition, 3 of the 4 B chromosomes also contained 45S rDNA copies. Silver nitrate staining in interphase nuclei provided indirect evidence of the expression of these rRNA genes in B chromosomes, indicating the probable origin of these elements. This report shows plasticity in the chromosomal localization of repeat DNA in B. westermanni and features a discussion of genomic diversification.

show abstract

Section: Discussionsupporting

confidence: 91%

Chromosomal Mapping of Repeat DNA in Bergiaria westermanni (Pimelodidae, Siluriformes): Localization of 45S rDNA in B Chromosomes

Malimpensa

Traldi²,

Toyama³

et al. 2018

Cytogenet Genome Res

View full text Add to dashboard Cite

show abstract

“…The karyotype and 5S rDNA-FISH results agreed with previous studies (Artoni et al, 2001;Diniz et al, 2009;Marquioni et al, 2013;Yano et al, 2016Yano et al, , 2017b. In all species, the double-FISH results demonstrate that U1 snRNA and 5S rRNA are colocalized in the ninth chromosome pair (Figure 3), including T. auritus, which belongs to the first lineage that diverged from the ancestor of the genus (Mariguela et al, 2016).…”

Section: Discussionsupporting

confidence: 89%

“…As expected, the transcribed region showed lower divergence among species, but even in that, T. auritus was the species with higher divergence. Besides nucleotide divergence, T. auritus also shows the most divergence cytogenetic data concerning number of 5S rDNA sites (Yano et al, 2017b). While all other species show one or two chromosome pairs bearing 5S rDNA sequences, T. auritus present 5 pairs (Figure 3B).…”

Section: Discussionmentioning

confidence: 89%

“…Triportheus has stood out in many cytogenetics studies, mainly because all species studied so far have a ZW sex chromosomes system well characterized (Artoni et al, 2001;Diniz et al, 2009;Yano et al, 2016Yano et al, , 2017a. In addition, the rDNAs (Diniz et al, 2009;Marquioni et al, 2013;Yano et al, 2017b) and the U2 snRNA genes (Yano et al, 2017b) have been also investigated, highlighting the variability in the syntenic configuration as well as in the number of sites of these multigene families, mainly regarding the 5S rDNAs (Yano et al, 2017b).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evolutionary Dynamics of Multigene Families in Triportheus (Characiformes, Triportheidae): A Transposon Mediated Mechanism?

et al. 2020

Self Cite

View full text Add to dashboard Cite

Triportheus (Characiformes, Triportheidae) is a freshwater fish genus with 18 valid species. These fishes are widely distributed in the major river drainages of South America, having commercial importance in the fishing market, mainly in the Amazon basin. This genus has diverged recently in a complex process of speciation carried out in different river basins. The use of repetitive sequences is suitable to trace the genomic reorganizations occured along the speciation process. In this work, the 5S rDNA multigene family has been characterized at molecular and phylogenetic level. The results showed that other multigene family has been found within the non-transcribed spacer (NTS): the U1 snRNA gene. Double-FISH with 5S and U1 probes were also performed, confirming the close linkage between these two multigene families. Moreover, evidences of different transposable elements (TE) were detected within the spacer, thus suggesting a transposon-mediated mechanism of 5S-U1 evolutionary pathway in this genus. Phylogenetic analysis demonstrated a species-specific grouping, except for Triportheus pantanensis, Triportheus aff. rotundatus and Triportheus trifurcatus. The evolutionary model of the 5S rDNA in Triportheus species has been discussed. In addition, the results suggest new clues for the speciation and evolutionary trend in these species, which could be suitable to use in other Characiformes species.

show abstract

“…Although scarce, the mapping of U2 snDNA sequences in different individuals has demonstrated that these sequences may be linked with other multigene families. According to Yano et al (2017), in four Triportheus Cope, 1872 species, the U2 snRNA genes are syntenic with both rDNAs (18S and 5S), while in Triportheus albus Cope, 1872, the U2 snRNA genes are syntenic with 18S rDNA and in other three Triportheus species, the U2 snRNA genes are not syntenic with rDNAs. The last described pattern is common in fish (Pelliccia et al, 2001;Manchado et al, 2006;Úbeda-Manzanaro et al, 2010;Utsunomia et al, 2014;Scacchetti et al, 2015;Silva et al, 2015).…”

Section: Original Articlementioning

confidence: 99%

Conserved number of U2 snDNA sites in Piabina argentea, Piabarchus stramineus and two Bryconamericus species (Characidae, Stevardiinae)

2018

View full text Add to dashboard Cite

The chromosomal location of 5S rRNA and U2 snRNA genes of Piabina argentea, Piabarchus stramineus and two Bryconamericus species from two different Brazilian river basins were investigated, in order to contribute to the understanding of evolutionary characteristics of these repetitive DNAs in the subfamily Stevardiinae. The diploid chromosome number was 2n = 52 for Bryconamericus cf. iheringii, Bryconamericus turiuba, Piabarchus stramineus and Piabina argentea. The 5S rDNA clusters were located on one chromosome pair in P. stramineus and B. cf. iheringii, and on two pairs in B. turiuba and P. argentea. The U2 snDNA clusters were located on the one pair in all species. Two-color FISH experiments showed that the co-localization between 5S rDNA and U2 snDNA in P. stramineus can represent a marker for this species. Thus, the present study demonstrated that the number of U2 snDNA clusters observed for the four species was conserved, but particular characteristics can be found in the genome of each species.A localização cromossômica dos genes de RNAr 5S e RNAsn U2 de Piabina argentea, Piabarchus stramineus e duas espécies de Bryconamericus provenientes de duas bacias hidrográficas foi investigada, com a intenção de contribuir com o entendimento de características evolutivas destes DNAs repetitivos na subfamília Stevardiinae. O número cromossômico diploide foi 2n = 52 para Bryconamericus cf. iheringii, Bryconamericus turiuba, Piabarchus stramineus e Piabina argentea. Os sítios de DNAr 5S foram localizados em um par cromossômico em P. stramineus e B. cf. iheringii, e em dois pares em B. turiuba e P. argentea. Os sítios de DNAsn U2 foram localizados em um par em todas as espécies. Experimentos de FISH com duas sondas mostraram que a co-localização entre os DNAr 5S e DNAsn U2 em P. stramineus pode representar um marcador para esta espécie. Portanto, o presente estudo demonstrou que o número de sítios de DNAsn U2 observado para as quatro espécies foi conservado, porém características particulares podem ser encontradas no genoma de cada espécie.Palavras-chave: DNA repetitivo, DNAr 5S, Cromossomo, Número diploide. Splicing.

show abstract

Evolutionary Dynamics of rDNAs and U2 Small Nuclear DNAs in Triportheus (Characiformes, Triportheidae): High Variability and Particular Syntenic Organization

Cited by 35 publications

References 56 publications

Chromosomal Mapping of Repeat DNA in <b><i>Bergiaria westermanni</i></b> (Pimelodidae, Siluriformes): Localization of 45S rDNA in B Chromosomes

Chromosomal Mapping of Repeat DNA in <b><i>Bergiaria westermanni</i></b> (Pimelodidae, Siluriformes): Localization of 45S rDNA in B Chromosomes

Evolutionary Dynamics of Multigene Families in Triportheus (Characiformes, Triportheidae): A Transposon Mediated Mechanism?

Conserved number of U2 snDNA sites in Piabina argentea, Piabarchus stramineus and two Bryconamericus species (Characidae, Stevardiinae)

Contact Info

Product

Resources

About