Evolutionary divergence of the rye Pm17 and Pm8 resistance genes reveals ancient diversity

Singh, Simarjeet; Hurni, Severine; Ruinelli, Michela; Brunner, Susanne; Sánchez‐Martín, Javier; Krukowski, Patricia; Peditto, David; Buchmann, Gabriele; Zbinden, Helen; Keller, Beat

doi:10.1007/s11103-018-0780-3

Cited by 98 publications

(79 citation statements)

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“…In wheat, arrays of powdery mildew R genes ( Pm genes and temporarily designated genes) and alleles have been documented over 30 years, and in this review we have provided an updated list based on current knowledge (Table ). Of these powdery mildew resistance genes documented in the wheat gene pool, only six ( Pm2 , Pm3 , Pm8 , Pm17 , Pm21 , and Pm60 ) have been cloned to date, all encoding proteins of the NLR class (Yahiaoui et al , 2004; Cao et al , 2011; Hurni et al , 2013; Sanchez‐Martin et al , 2016; Xing et al , 2017; He et al , 2018; Singh et al , 2018; Zou et al , 2018). The gene‐for‐gene interaction always results in the host having a full resistance rating to the pathogen.…”

Section: Resistance Types For Powdery Mildewmentioning

confidence: 99%

“…These 17 alleles share 97% homology; the Pm3h , Pm3i , and Pm3j alleles are identical to Pm3d , Pm3c, and Pm3b , respectively (Bougot et al , 2002; Huang et al , 2004; Yahiaoui et al , 2006; Bhullar et al , 2009; 2010b). A recent comparative analysis showed that the UP3 sequence, a 184 bp fragment that specifically detects Pm3 loci (Yahiaoui et al , 2004; Hurni et al , 2013), was highly conserved in Pm3 alleles as well as in two rye ( Secale cereale )‐derived wheat powdery mildew race‐specific resistance genes Pm17 and Pm8 (Singh et al , 2018). This suggests Pm17 and Pm8 from the 1RS translocation are putatively allelic and are orthologues of Pm3 .…”

Section: Resistance Types For Powdery Mildewmentioning

confidence: 99%

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Mechanisms of powdery mildew resistance of wheat – a review of molecular breeding

et al. 2020

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Powdery mildew (Blumeria graminis f. sp. tritici) results in serious economic loss in wheat production. Exploration of plant resistance to wheat powdery mildew over several decades has led to the discovery of a wealth of resistance genes and quantitative trait loci (QTLs). We have provided a comprehensive summary of over 200 powdery mildew genes (permanently and temporarily designated genes) and QTLs reported in common bread wheat. This highlights the diverse and rich resistance sources that exist across all 21 chromosomes. To manage different data for breeders, here we also present a bridged mapping result from previously reported powdery mildew resistance genes and QTLs with the application of a published integrated wheat map. This will provide important insights to empower further breeding of powdery mildew resistant wheat via marker‐assisted selection (MAS).

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Section: Resistance Types For Powdery Mildewmentioning

confidence: 99%

Section: Resistance Types For Powdery Mildewmentioning

confidence: 99%

Mechanisms of powdery mildew resistance of wheat – a review of molecular breeding

et al. 2020

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“…A small number of these genes have been cloned and most encode nucleotide‐binding, leucine‐rich repeat (NLR) immune receptors, i.e. Pm2 (Sánchez‐Martín et al ., 2016), the Pm3 allelic series (Brunner et al ., 2010), Pm5e (Xie et al ., 2020), Pm8 (Hurni et al ., 2013), Pm17 (Singh et al ., 2018), Pm21 (He et al ., 2018; Xing et al ., 2018), Pm41 (M. Li et al ., 2020) and Pm60 (Zou et al ., 2018). However, a tandem kinase‐pseudokinase, Pm24 (Lu et al ., 2020), also provides powdery mildew resistance as do broad‐spectrum adult plant resistance genes Pm38 and Pm46 that each provide partial resistance to powdery mildew and rust diseases in adult plants and encode an ABC transporter and an altered sugar transporter, respectively (Krattinger et al ., 2009; Moore et al ., 2015).…”

Section: Introductionmentioning

confidence: 99%

A highly differentiated region of wheat chromosome 7AL encodes a Pm1a immune receptor that recognizes its corresponding AvrPm1a effector from Blumeria graminis

Hewitt

Müller²,

Molnár

et al. 2020

New Phytologist

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 Pm1a, the first powdery mildew resistance gene described in wheat, is part of a complex resistance (R) gene cluster located in a distal region of chromosome 7AL that has suppressed genetic recombination.  A nucleotide-binding, leucine-rich repeat (NLR) immune receptor gene was isolated using mutagenesis and R gene enrichment sequencing (MutRenSeq). Stable transformation confirmed Pm1a identity which induced a strong resistance phenotype in transgenic plants upon challenge with avirulent Blumeria graminis (wheat powdery mildew) pathogens.  A high-density genetic map of a B. graminis family segregating for Pm1a avirulence combined with pathogen genome resequencing and RNAseq identified AvrPm1a effector gene candidates. In planta expression identified an effector, with an N terminal Y/FxC motif, that induced a strong hypersensitive response when co-expressed with Pm1a in Nicotiana benthamiana.  Single chromosome enrichment sequencing (ChromSeq) and assembly of chromosome 7A suggested that suppressed recombination around the Pm1a region was due to a rearrangement involving chromosomes 7A, 7B and 7D. The cloning of Pm1a and its identification in a highly rearranged region of chromosome 7A provides insight into the role of chromosomal rearrangements in the evolution of this complex resistance cluster.

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“…6RL from rye cv. Prolific [9,10], JZHM [13], German White [11] and Kustro [14] have powdery mildew resistance genes. Hao et al [16] reported powdery mildew resistance gene Pm56 on 6RS from rye cv.…”

Section: Discussionmentioning

confidence: 99%

“…Two of the most successful translocations were 1BL.1RS and 1AL.1RS, which carry powdery mildew genes Pm8 and Pm17, respectively. Further molecular clarification of Pm8 and Pm17 revealed that they were orthologous resistance genes with different evolutionary history [10,11]. Friebe et al [12] localized the powdery mildew resistance gene Pm20, which was derived from the chromosome 6RL of S. cereale cv.…”

Section: Introductionmentioning

confidence: 99%

Molecular dissection of Secale africanum chromosome 6Rafr in wheat enabled localization of genes for resistance to powdery mildew and stripe rust

et al. 2020

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Background: Introgression of chromatin from Secale species into common wheat has for decades been a successful strategy for controlling the wheat diseases. The wild Secale species, Secale africanum Stapf., is a valuable source for resistance to foliar disease of wheat. A wheat-S. africanum chromosome 6R afr substitution line displayed resistance to both powdery mildew and stripe rust at the adult-plant stage. Results: Wheat-S. africanum chromosome 6R afr deletion and translocation lines were produced and identified by sequential non-denaturing fluorescence in situ hybridization (ND-FISH) using multiple Oligo-based probes. Different ND-FISH patterns were observed between S. cereale 6R and S. africanum 6R afr. With reference to the physical map of the draft genome sequence of rye inbred line Lo7, a comprehensive PCR marker analysis indicated that insertions and deletions had occurred by random exchange between chromosomes 6R and 6R afr. A survey of the wheat-S. africanum 6R afr lines for disease resistance indicated that a powdery mildew resistance gene(s) was present on the long arm of 6R afr at FL0.85-1.00, and that a stripe rust resistance gene(s) was located in the terminal region of 6R afr S at FL0.95-1.00. The wheat-S. africanum 6R afr introgression lines also displayed superior agronomic traits, indicating that the chromosome 6R afr may have little linkage drag in the wheat background. Conclusions: The combination of molecular and cytogenetic methods allowed to precisely identify the chromosome rearrangements in wheat-S. africanum 6R afr substitution, deletion and translocation lines, and compare the structural difference between chromosomes 6R and 6R afr. The wheat-S. africanum 6R afr lines containing gene(s) for powdery mildew and stripe rust resistance could be used as novel germplasm for wheat breeding by chromosome engineering.

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Evolutionary divergence of the rye Pm17 and Pm8 resistance genes reveals ancient diversity

Cited by 98 publications

References 57 publications

Mechanisms of powdery mildew resistance of wheat – a review of molecular breeding

Mechanisms of powdery mildew resistance of wheat – a review of molecular breeding

A highly differentiated region of wheat chromosome 7AL encodes a Pm1a immune receptor that recognizes its corresponding AvrPm1a effector from Blumeria graminis

Molecular dissection of Secale africanum chromosome 6Rafr in wheat enabled localization of genes for resistance to powdery mildew and stripe rust

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