Evolutionary Divergence of the Mouse and Human Lgn1/SMA Repeat Structures

Growney, Joseph D.; Scharf, Jeremiah M.; Kunkel, Louis M.; Dietrich, William F.

doi:10.1006/geno.1999.6111

Cited by 24 publications

(15 citation statements)

References 70 publications

(143 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These include the "unique" Naip genes on the borders, Naip2 and Naip1, as well as four of the five Naip genes that lie in the central amplification, namely Naip5, Naip7, Naip6, and Naip3 ( Fig. 1; Growney et al 2000). Our analysis of these genomic sequences indicates that intron 13, which is present in all copies of Naip, exhibits gene-specific polymorphisms and can be grouped into two classes.…”

Section: Comparison Of Naip Repeat Structure Among Inbred Mouse Strainsmentioning

confidence: 80%

“…However, up to this point, we have not been able precisely to precisely physically map these recombinant loci relative to the genes in the Naip array (Scharf et al 1996;Endrizzi et al 1999;Growney et al 2000). The C57BL/6J map allowed us to physically localize each recombinant locus in the Naip array.…”

Section: Improved Genetic Mapping Of Lgn1mentioning

confidence: 98%

“…We identified 21 clones from two distinct BAC libraries (see Methods; Table 2). We ordered the clones into a complete physical map of the C57BL/6J Naip array using markers developed in mapping the 129 interval (Table 1) (Dietrich et al 1994(Dietrich et al , 1995Scharf et al 1996Scharf et al , 1998Endrizzi et al 1999;Growney et al 2000). Our reasoning behind our ability to order the clones and markers is outlined as follows.…”

Section: Construction Of C57bl/6j Physical Mapmentioning

confidence: 99%

“…Distinct genes present in both intervals have been attributed to the primary effects of each phenotype. While one or more closely related Naip (neuronal apoptosis inhibitory protein) paralogs has been identified as a candidate gene for Lgn1 Growney et al 2000), SMN is responsible for the majority of SMA cases Rodrigues et al 1995). However, Naip remains a candidate modifying gene for SMA disease severity (Roy et al 1995;Morrison 1996;Chang et al 1997).…”

mentioning

confidence: 99%

“…However, polymorphisms between the strains used in our genetic mapping (A/J and C57BL/6J) and the strains used in our physical mapping (129) have prevented us from refining the Lgn1 genetic interval within the central portions of the Naip array (Dietrich et al 1995;Scharf et al 1996;Endrizzi et al 1999;Growney et al 2000). Because we have several animals from our cross that are recombinant within the Naip array, it seemed likely that additional attempts to physically map the location of recombinant genetic markers contained in the central repetitive Naip array would allow us to improve our genetic mapping of Lgn1.…”

mentioning

confidence: 99%

See 4 more Smart Citations

High-resolution Genetic and Physical Map of theLgn1Interval in C57BL/6J ImplicatesNaip2orNaip5inLegionella pneumophilaPathogenesis

Growney¹,

Dietrich²

2000

Genome Res.

Self Cite

View full text Add to dashboard Cite

Prior genetic and physical mapping has shown that theNaip gene cluster on mouse chromosome 13D1-D3 contains a gene, Lgn1, that is responsible for determining the permissivity of ex vivo macrophages to Legionella pneumophila replication. We have identified differences in the structure of the Naip array among commonly used inbred mouse strains, although these gross structural differences do not correlate with differences in L. pneumophilapermissiveness. A physical map of the region employing clones of the C57BL/6J haplotype confirms that there are fewer copies ofNaip in this strain than are in the physical map of the 129 haplotype. We have also refined the genetic location ofLgn1, leaving only Naip2 and Naip5as candidates for Lgn1. Our genetic map suggests the presence of two hotspots of recombination within the Naiparray, indicating that the 3′ portion of Naip may be involved in the genomic instability at this locus.[The sequence data described in this paper have been submitted to the GenBank data library under accession nos. AF240489–AF240530.]

show abstract

Section: Comparison Of Naip Repeat Structure Among Inbred Mouse Strainsmentioning

confidence: 80%

Section: Improved Genetic Mapping Of Lgn1mentioning

confidence: 98%

Section: Construction Of C57bl/6j Physical Mapmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

High-resolution Genetic and Physical Map of theLgn1Interval in C57BL/6J ImplicatesNaip2orNaip5inLegionella pneumophilaPathogenesis

Growney¹,

Dietrich²

2000

Genome Res.

Self Cite

View full text Add to dashboard Cite

show abstract

NAIPs: Building an innate immune barrier against bacterial pathogens

Kofoed

Vance

2012

BioEssays

View full text Add to dashboard Cite

The innate immune system of mammals encodes several families of immune detector proteins that monitor the cytosol for signs of pathogen invasion. One important but poorly understood family of cytosolic immunosurveillance proteins is the NLR (nucleotide-binding domain, leucine-rich repeat containing) proteins. Recent work has demonstrated that one subfamily of NLRs, the NAIPs (NLR family, apoptosis inhibitory proteins), are activated by specific interaction with bacterial ligands, such as flagellin. NAIP activation leads to assembly of a large multiprotein complex called the inflammasome, which initiates innate immune responses by activation of the Caspase-1 protease. NAIPs therefore appear to detect pathogen molecules via a simple and direct receptor-ligand mechanism. Interestingly, other NLR family members appear to detect pathogens indirectly, perhaps by responding to host cell "stress" caused by the pathogen. Thus, the NLR family may have evolved surprisingly diverse mechanisms for detecting pathogens.

show abstract