Evolutionary and biogeographic history of the black fly Simulium wayani (Diptera: Simuliidae) on the island of Timor

Adler, Peter H.; Takaoka, Hiroyuki; Sofian‐Azirun, Mohd; Suana, I Wayan

doi:10.1016/j.actatropica.2019.02.017

Cited by 2 publications

(5 citation statements)

References 20 publications

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“…Simulium (N.) wayani has four pupal gill filaments. Our results support the hypothesis that S. (N.) wayani might have evolved from an ancestral six-filamented population of S. (N.) aureohirtum, proposed by Takaoka, Sofian-Azirun [52], perhaps along with members of the S. (N.) ornatipes complex [1].…”

Section: Discussionsupporting

confidence: 90%

“…The sequences of S. ( N. ) wayani were nested within one of the S. ( N. ) aureohirtum subgroups with low genetic distances (minimum = 0.66%), although S. ( N. ) aureohirtum is readily distinguished from S. ( N. ) wayani by the number of pupal gill filaments, suggesting that S. ( N. ) wayani is closely related to the S. ( N. ) aureohirtum subgroup. Chromosomal analyses indicate, however, that S. ( N. ) wayani is closely related to the S. ( N. ) ornatipes complex of mainland Australia [ 1 ], indicating that further barcode studies should include the S. ( N. ) ornatipes complex. Takaoka [ 51 ] inferred that species of the S. ruficorne group dispersed eastward from Sumatra in Indonesia to the Australasian Region while reducing the pupal gill filaments from eight ( S. ( N. ) glattharri Takaoka & Davies) to four ( S. ( N. ) ornatipes ) through six ( S. ( N. ) aureohirtum ).…”

Section: Discussionmentioning

confidence: 99%

“…Author details 1 Tropical Infectious Diseases Research and Education Centre (TIDREC), Universiti Malaya, Kuala Lumpur, Malaysia. 2 Institute for Advanced Studies, Universiti Malaya, Kuala Lumpur, Malaysia.…”

Section: Abbreviationsmentioning

confidence: 99%

“…Black flies (Diptera: Simuliidae) are medically important haematophagous insects for humans, domestic animals and wildlife, due to their pestiferous biting habits and vectorial roles in transmitting various parasites. They are the sole vector of the filarial nematode Onchocerca volvulus , which causes river blindness, the second leading infectious cause of blindness in the world [ 1 ]. They also transmit other Onchocerca species, Mansonella filarial parasites and Leucocytozoon and Trypanosoma protozoa [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…1 Simulium rangatense is represented by a type specimen 2 Specimens were collected from type localities making the S. timorense clade paraphyletic. However, in the BI tree, the S. nobile and S. timorense clades were well separated.…”

mentioning

confidence: 99%

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DNA barcoding of black flies (Diptera: Simuliidae) in Indonesia

Hew,

Ya’cob,

Adler

et al. 2023

Parasites Vectors

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Background DNA barcoding is a valuable taxonomic tool for rapid and accurate species identification and cryptic species discovery in black flies. Indonesia has 143 nominal species of black flies, but information on their biological aspects, including vectorial capacity and biting habits, remains underreported, in part because of identification problems. The current study represents the first comprehensive DNA barcoding of Indonesian black flies using mitochondrial cytochrome c oxidase subunit I (COI) gene sequences. Methods Genomic DNA of Indonesian black fly samples were extracted and sequenced, producing 86 COI sequences in total. Two hundred four COI sequences, including 118 GenBank sequences, were analysed. Maximum likelihood (ML) and Bayesian inference (BI) trees were constructed and species delimitation analyses, including ASAP, GMYC and single PTP, were performed to determine whether the species of Indonesian black flies could be delineated. Intra- and interspecific genetic distances were also calculated and the efficacy of COI sequences for species identification was tested. Results The DNA barcodes successfully distinguished most morphologically distinct species (> 80% of sampled taxa). Nonetheless, high maximum intraspecific distances (3.32–13.94%) in 11 species suggested cryptic diversity. Notably, populations of the common taxa Simulium (Gomphostilbia) cheongi, S. (Gomphostilbia) sheilae, S. (Nevermannia) feuerborni and S. (Simulium) tani in the islands of Indonesia were genetically distinct from those on the Southeast Asian mainland (Malaysia and Thailand). Integrated morphological, cytogenetic and nuclear DNA studies are warranted to clarify the taxonomic status of these more complex taxa. Conclusions The findings showed that COI barcoding is a promising taxonomic tool for Indonesian black flies. The DNA barcodes will aid in correct identification and genetic study of Indonesian black flies, which will be helpful in the control and management of potential vector species. Graphical abstract

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%