Evolutionary Analysis of MBW Function by Phenotypic Rescue in Arabidopsis thaliana

Zhang, Bipei; Hülskamp, Martin

doi:10.3389/fpls.2019.00375

Cited by 27 publications

(16 citation statements)

References 65 publications

(104 reference statements)

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“…For example, the meristem identity transition transcription factor gene, LMI2 (Pastore et al, 2011), resides near a DHS that was deactivated during seed coat cell maturation ( Figure 1B ). Similar to previous observations (Sullivan et al, 2014), the majority of observed DHSs were static during development, such as those flanking TTG1 , which encodes a WDR protein that regulates seed coat mucilage release (Zhang and Hülskamp, 2019) ( Figure 1B ). The regulatory landscape of seed coat cells differed significantly from the landscape of root nonhair cells, another epidermal cell type, as well as from whole roots ( Figures 1B and 5 ).…”

Section: Resultssupporting

confidence: 88%

Mapping and Dynamics of Regulatory DNA in Maturing Arabidopsis thaliana Siliques

et al. 2019

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The genome is reprogrammed during development to produce diverse cell types, largely through altered expression and activity of key transcription factors. The accessibility and critical functions of epidermal cells have made them a model for connecting transcriptional events to development in a range of model systems. In Arabidopsis thaliana and many other plants, fertilization triggers differentiation of specialized epidermal seed coat cells that have a unique morphology caused by large extracellular deposits of polysaccharides. Here, we used DNase I-seq to generate regulatory landscapes of A. thaliana seeds at two critical time points in seed coat maturation (4 and 7 DPA), enriching for seed coat cells with the INTACT method. We found over 3,000 developmentally dynamic regulatory DNA elements and explored their relationship with nearby gene expression. The dynamic regulatory elements were enriched for motifs for several transcription factors families; most notably the TCP family at the earlier time point and the MYB family at the later one. To assess the extent to which the observed regulatory sites in seeds added to previously known regulatory sites in A. thaliana, we compared our data to 11 other data sets generated with 7-day-old seedlings for diverse tissues and conditions. Surprisingly, over a quarter of the regulatory, i.e. accessible, bases observed in seeds were novel. Notably, plant regulatory landscapes from different tissues, cell types, or developmental stages were more dynamic than those generated from bulk tissue in response to environmental perturbations, highlighting the importance of extending studies of regulatory DNA to single tissues and cell types during development.

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Section: Resultssupporting

confidence: 88%

Mapping and Dynamics of Regulatory DNA in Maturing Arabidopsis thaliana Siliques

et al. 2019

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“…In addition to the regulation of the flavonoid pathway, genes of the regulatory complex MBW are also responsible for a number of functions in the plant organism [52], for example, such as the formation of spines analyzed by us [27]. Therefore, the MBW genes, especially bHLH and MYB, are highly variable [47].…”

Section: Discussionmentioning

confidence: 99%

The Development of the Genic SSR Markers for Analysis of Genetic Diversity in Gooseberry Cultivars

et al. 2021

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Gooseberry is one of the most promising and underrated small fruit crops. There is a need to improve and genotype the existing cultivars, and this work requires the use of new efficient DNA marking techniques. Marker-assisted selection (MAS) is a modern approach for work with genetic resources. However, the genetic determinants of key qualitative traits are poorly studied. Therefore, we developed SSR markers located in flavonoid biosynthesis genes that can affect the resistance of plants to biotic and abiotic stresses to study the genetic diversity of gooseberry cultivars grown in the Russian Federation and varying in genetic and geographical origin. We have genotyped 22 gooseberry cultivars using a set of 19 of our original microsatellite markers and two neutral previously published ones. A total of 53 alleles were found. Nine of the 21 analyzed loci were polymorphic. The most polymorphic loci of flavonoid biosynthesis were found in the DFR gene (RucDFR1-2, RucDFR1-3, RucDFR2-1), their mean PIC (polymorphism information content) being 0.65, comparable to the PIC values of neutral markers. Our set of gene-targeted SSR markers showed that most of all the studied gooseberry cultivars differed in origin, based on which they were divided into three groups: European cultivars, Russian cultivars, and interspecies hybrids. Thus, the polymorphic markers can be used for cultivar identification and certification as well as for the marker-assisted selection of gooseberry plants having different origins and phenotypic traits.

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“…As indicated in the name, MBW, the complex is generally comprised of three distinguished transcription factors including MYB, bHLH and WDR [reviewed in Xu et al., ]. Based on the characterizations of different Arabidopsis mutants at phenotypic, genetic and molecular levels, the MBW complex is known to regulate two groups of traits in this model plant including: (1) plant tissue colour (anthocyanin biosynthesis and seed coat colour) and (2) epidermal cell fate determination (root hair and trichome morphogenesis) [Zhang and Hulskamp, ; Shibata and Sugimoto, ]. In Arabidopsis, MYB is a large gene family which contains more than 180 members [Yanhui et al., ; Dubos et al., ; Nguyen et al., ].…”

Section: Mbw Complexmentioning

confidence: 99%

“…The bHLH transcription factors can be TRANSPARENT TESTA8 (TT8), GLABROUS3 (GL3), ENHANCER OF GLABRA3 (EGL3) or MYC1 [Zhang et al., ; Baudry et al., ; Gou et al., ; Pesch et al., ]. Although there are several MYB and bHLH transcription factors in each group, the WDR is only encoded by one single copy gene, TRANSPARENT TESTA GLABRA1 ( TTG1 ) [Koornneef, ; Nguyen et al., ; Zhang and Hulskamp, ]. The DNA‐binding proteins (MYB and bHLH) together with TTG1 comprise a tertiary transcription factor complex which can generally activate the downstream genes to regulate the mentioned traits in Arabidopsis plant [Xu et al., ; Miller et al., ; Zhang and Hulskamp, ].…”

Section: Mbw Complexmentioning

confidence: 99%

The MYB–bHLH–WDR interferers (MBWi) epigenetically suppress the MBW's targets

Nguyen

Tran

Nguyen

2019

Biology of the Cell

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Active repressors have been evidenced to function in different plant growth and development programs including hormonal signalling pathways. In Arabidopsis, the MYB-bHLH-WDR (MBW) complex is known to regulate different phenotypic traits such as anthocyanin biosynthesis, seed coat colour, trichome and root hair patterning. A number of transcription factors have been identified to play a negative role in the regulation of these traits via the interruption of MBW formation and function. Since these transcription factors work to interfere with the MBW complex, this review suggests their general name as MBW interferers (MBWi). Recent studies have shed light on the molecular mechanism of these MBWi and this review is aiming to provide a precise view of these MBWi. Moreover, from these, a new characteristic of active repressors is also updated. 284www.biolcell.net | Volume (111) | Pages 284-291 285

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Evolutionary Analysis of MBW Function by Phenotypic Rescue in Arabidopsis thaliana

Cited by 27 publications

References 65 publications

Mapping and Dynamics of Regulatory DNA in Maturing Arabidopsis thaliana Siliques

Mapping and Dynamics of Regulatory DNA in Maturing Arabidopsis thaliana Siliques

The Development of the Genic SSR Markers for Analysis of Genetic Diversity in Gooseberry Cultivars

The MYB–bHLH–WDR interferers (MBWi) epigenetically suppress the MBW's targets

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