Evolutionarily conserved function of the sacred lotus (Nelumbo nucifera Gaertn.) CER2-LIKE family in very-long-chain fatty acid elongation

Yang, Xianpeng; Wang, Zhou-Ya; Feng, Tao; Li, Juanjuan; Huang, Longyu; Yang, Baiming; Zhao, Huayan; Jenks, Matthew A.; Yang, Pingfang; Lü, Shiyou

doi:10.1007/s00425-018-2934-6

Cited by 11 publications

(6 citation statements)

References 68 publications

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“…Due to its low accumulation of C 22 and C 24 VLCFAs [ 34 ], the yeast mutant strain BY4741 Δfah1 was used to investigate the function of KCS20. The CsKCS20 protein was expressed in BY4741 Δfah1 yeast, and the yeast fatty acyl chains were quantified by gas chromatography–mass spectrometry (GC–MS) ( Fig.…”

Section: Resultsmentioning

confidence: 99%

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Function and transcriptional regulation of CsKCS20 in the elongation of very-long-chain fatty acids and wax biosynthesis in Citrus sinensis flavedo

Wang

Yang

Chen

et al. 2022

Horticulture Research

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Cuticular wax on plant aerial surfaces plays a vital role in the defense against various stresses, and the genes related to wax metabolism have been well documented in several model plants. However, there is very limited research on the key enzymes and transcription factors (TFs) associated with carbon chain distribution and wax biosynthesis in citrus fruit. In this study, an analysis of wax metabolites indicated that even carbon-chain (C24-C28) metabolites are the dominant wax components in citrus fruit, and a 3-ketoacyl-CoA synthase (KCS) family gene (CsKCS20) plays an important role in the carbon chain distribution during wax biosynthesis in a wax-deficient mutant (MT). Expression of CsKCS20 in yeast indicated that CsKCS20 can catalyze the biosynthesis of C22 and C24 very-long-chain fatty acids (VLCFAs). In addition, transcriptome and sequence analysis indicated that the differential expression of CsKCS20 between the wild-type (WT) and MT fruit can be partly attributed to the regulation of CsMYB96, which was further confirmed by yeast one-hybrid (Y1H) assays, electrophoretic mobility shift assays (EMSAs) and dual luciferase assays. The functions of CsMYB96 and CsKCS20 in wax biosynthesis were further validated by heterologous expression in Arabidopsis. In summary, this study elucidates the important roles of CsKCS20 and CsMYB96 in regulating VLCFA elongation and cuticular wax biosynthesis, which provides new directions for the improvement of citrus fruit wax quality in genetic breeding programs.

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Section: Resultsmentioning

confidence: 99%

“…The coding sequence of CsKCS20 was obtained from cDNA from “Newhall” navel orange and cloned into the p426 yeast expression vector. The fatty acid methyl esters (FAMEs) of the yeast strain were analyzed as described by Yang et al [ 34 ]. Individual FAMEs were determined by the characteristic ion fragments.…”

Section: Methodsmentioning

confidence: 99%

Function and transcriptional regulation of CsKCS20 in the elongation of very-long-chain fatty acids and wax biosynthesis in Citrus sinensis flavedo

Wang

Yang

Chen

et al. 2022

Horticulture Research

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“…A. thaliana has 21 KCS genes (Joubès et al., 2008). Characterization of the members of the KCS family showed their diverse chain‐length specificities (Yang et al., 2018). We identified KCS4 to be differentially regulated during PZ progression.…”

Section: Discussionmentioning

confidence: 99%

Glycerolipid profile differences between perennial and annual stem zones in the perennial model plant Arabis alpina

et al. 2021

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The perennial life style is a successful ecological strategy, and Arabis alpina is a recently developed model Brassicaceae species for studying it. One aspect, poorly investigated until today, concerns the differing patterns of allocation, storage, and metabolism of nutrients between perennials and annuals and the yet unknown signals that regulate this process. A. alpina has a complex lateral stem architecture with a proximal vegetative perennial (PZ) and a distal annual flowering zone (AZ) inside the same stems. Lipid bodies (LBs) with triacylglycerols (TAGs) accumulate in the PZ. To identify potential processes of lipid metabolism linked with the perennial lifestyle, we analyzed lipid species in the PZ versus AZ. Glycerolipid fractions, including neutral lipids with mainly TAGs, phospholipids, and glycolipids, were present at higher levels in the PZ as compared to AZ or roots. Concomitantly, contents of specific long‐chain and very long‐chain fatty acids increased during formation of the PZ. Corresponding gene expression data, gene ontology term enrichment, and correlation analysis with lipid species pinpoint glycerolipid‐related genes to be active during the development of the PZ. Possibilities that lipid metabolism genes may be targets of regulatory mechanisms specifying PZ differentiation in A. alpina are discussed.

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“…However, heterologous expression of KCS6 in yeast was found to only produce fatty acids up to 28 carbons [ 45 ]. The characterization of the CER2-LIKEs gene family in arabidopsis, but also in rice, lotus, maize or poplar, has revealed that proteins encoded by these genes physically interact with the FAE components and play a key role in specifically modifying the chain-length specificity of KCS6, enabling the production of VLCFA longer than 28 carbons [ 40 , 42 , 70 , 71 , 72 , 73 ].…”

Section: Vlcfa-derived Surface Lipids Constitute the Border Between Plants And Its Surrounding Environmentmentioning

confidence: 99%

Biosynthesis and Functions of Very-Long-Chain Fatty Acids in the Responses of Plants to Abiotic and Biotic Stresses

Batsale

Bahammou

Fouillen

et al. 2021

Cells

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Very-long-chain fatty acids (i.e., fatty acids with more than 18 carbon atoms; VLCFA) are important molecules that play crucial physiological and structural roles in plants. VLCFA are specifically present in several membrane lipids and essential for membrane homeostasis. Their specific accumulation in the sphingolipids of the plasma membrane outer leaflet is of primordial importance for its correct functioning in intercellular communication. VLCFA are found in phospholipids, notably in phosphatidylserine and phosphatidylethanolamine, where they could play a role in membrane domain organization and interleaflet coupling. In epidermal cells, VLCFA are precursors of the cuticular waxes of the plant cuticle, which are of primary importance for many interactions of the plant with its surrounding environment. VLCFA are also major components of the root suberin barrier, which has been shown to be fundamental for nutrient homeostasis and plant adaptation to adverse conditions. Finally, some plants store VLCFA in the triacylglycerols of their seeds so that they later play a pivotal role in seed germination. In this review, taking advantage of the many studies conducted using Arabidopsis thaliana as a model, we present our current knowledge on the biosynthesis and regulation of VLCFA in plants, and on the various functions that VLCFA and their derivatives play in the interactions of plants with their abiotic and biotic environment.

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Evolutionarily conserved function of the sacred lotus (Nelumbo nucifera Gaertn.) CER2-LIKE family in very-long-chain fatty acid elongation

Cited by 11 publications

References 68 publications

Function and transcriptional regulation of CsKCS20 in the elongation of very-long-chain fatty acids and wax biosynthesis in Citrus sinensis flavedo

Function and transcriptional regulation of CsKCS20 in the elongation of very-long-chain fatty acids and wax biosynthesis in Citrus sinensis flavedo

Glycerolipid profile differences between perennial and annual stem zones in the perennial model plant Arabis alpina

Biosynthesis and Functions of Very-Long-Chain Fatty Acids in the Responses of Plants to Abiotic and Biotic Stresses

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