Evolution of Tre-2/Bub2/Cdc16 (TBC) Rab GTPase-activating proteins

Gabernet-Castello, Carme; O’Reilly, Amanda; Dacks, Joel B.; Field, Mark C.

doi:10.1091/mbc.e12-07-0557

Cited by 53 publications

(59 citation statements)

References 56 publications

(82 reference statements)

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“…Additional examples include members of the Rab GTPase GAPs, the TBC family. Similar to AGAP, many of the TBC subfamilies (i.e., TBC-F through TBC-N) are found in multiple supergroups, but also seem to be absent from the majority of taxa (Elias et al 2012;Gabernet-Castello et al 2013). More strikingly, there are Rab GAPs and Rab GTPases, for example, TBC-RootA and RabTitan, respectively (Fig.…”

Section: Evolution Of the Endomembrane Systemmentioning

confidence: 93%

“…For example, the major coat proteins (COPI, COPII, clathrin, adaptins, retromer), Arf GAPs, the small GTPases (Arfs, Sar, Rabs), Syntaxins, EpsinR, and ESCRTs are all found in diverse eukaryotes, indicating once again that the LECA possessed a membranetrafficking system at least as complex as that of most living eukaryotes. Phylogenetic analysis of paralogous protein families such as Syntaxins, Longins, Adaptins, Rabs, Arf GAPs, and TBCs has shown broad conservation of organelle-specific paralogs Doolittle 2002, 2004;Vedovato et al 2009;Hirst et al 2011;Elias et al 2012;Gabernet-Castello et al 2013) suggesting that these are a part of the plesiomorphic state of eukaryotes, adding depth to the deduced level of reconstructed complexity of a membrane-trafficking system in the LECA.…”

Section: A Sophisticated Ancient Membrane-trafficking Machinery and Amentioning

confidence: 99%

“…Examples here ( Fig. 2A) include the Rab GAP TBC-ExA in excavates, TBC-PlA and TBC-PlB in plants, and many additional lineage-specific Rab paralogs (Elias et al 2012;Gabernet-Castello et al 2013). Moreover, as molecular cell biological investigations in nonopisthokont models become more sophisticated and depart from the simple validation of opisthokont models, exciting examples are being found in the other supergroups as well.…”

Section: Evolution Of the Endomembrane Systemmentioning

confidence: 99%

See 2 more Smart Citations

Missing Pieces of an Ancient Puzzle: Evolution of the Eukaryotic Membrane-Trafficking System

Schlacht¹,

Herman²,

Klute³

et al. 2014

Cold Spring Harbor Perspectives in Biology

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The membrane-trafficking system underpins cellular trafficking of material in eukaryotes and its evolution would have been a watershed in eukaryogenesis. Evolutionary cell biological studies have been unraveling the history of proteins responsible for vesicle transport and organelle identity revealing both highly conserved components and lineage-specific innovations. Recently, endomembrane components with a broad, but patchy, distribution have been observed as well, pieces that are missing from our cell biological and evolutionary models of membrane trafficking. These data together allow for new insights into the history and forces that shape the evolution of this critical cell biological system.

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Section: Evolution Of the Endomembrane Systemmentioning

confidence: 93%

Section: A Sophisticated Ancient Membrane-trafficking Machinery and Amentioning

confidence: 99%

Section: Evolution Of the Endomembrane Systemmentioning

confidence: 99%

See 1 more Smart Citation

Missing Pieces of an Ancient Puzzle: Evolution of the Eukaryotic Membrane-Trafficking System

Schlacht¹,

Herman²,

Klute³

et al. 2014

Cold Spring Harbor Perspectives in Biology

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“…The GAPs for Rab GTPases contain a TBC Rab-binding domain. 52 Shigella flexneri can resist autophagy-mediated defense in infected epithelial cells and translocates the effector VirA, which is vital for intracellular bacterial persistence, into host cells by a type III secretion apparatus. 53 A recent study 54 demonstrated that VirA possesses TBC-like GAP activity for Rab1, and also found that deletion of VirA and deficiency of GAP activity in Shigella flexneri mutants caused 420% of infected cells to form autophagosomes containing intracellular bacteria.…”

mentioning

confidence: 99%

Regulation of autophagy by the Rab GTPase network

2014

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Autophagy (macroautophagy) is a highly conserved intracellular and lysosome-dependent degradation process in which autophagic substrates are enclosed and degraded by a double-membrane vesicular structure in a continuous and dynamic vesicle transport process. The Rab protein is a small GTPase that belongs to the Ras-like GTPase superfamily and regulates the vesicle traffic process. Numerous Rab proteins have been shown to be involved in various stages of autophagy. Rab1, Rab5, Rab7, Rab9A, Rab11, Rab23, Rab32, and Rab33B participate in autophagosome formation, whereas Rab9 is required in non-canonical autophagy. Rab7, Rab8B, and Rab24 have a key role in autophagosome maturation. Rab8A and Rab25 are also involved in autophagy, but their role is unknown. Here, we summarize new findings regarding the involvement of Rabs in autophagy and provide insights regarding future research on the mechanisms of autophagy regulation.

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“…1) have recently been reported. Analysis of both Rab proteins and their TBC-GAPs identified several uncharacterized Rab and Rab-GAP homologs that are ancient but without homologs in humans (Elias et al 2012;Gabernet-Castello et al 2013). Finally, an analysis of the ArfGAP protein family (Schlacht et al 2013) expanded the number of subfamilies from 10 to 11, with the new subfamily (ArfGAPC2) being absent from humans but present in diverse eukaryotic lineages, including plants and important agricultural pathogens like oomycetes.…”

Section: Evolution and Cell Biology Of The Endocytic Systemmentioning

confidence: 99%

The Cell Biology of the Endocytic System from an Evolutionary Perspective

Wideman

Leung

Field

et al. 2014

Cold Spring Harbor Perspectives in Biology

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Evolutionary cell biology can afford an interdisciplinary comparative view that gives insights into both the functioning of modern cells and the origins of cellular systems, including the endocytic organelles. Here, we explore several recent evolutionary cell biology studies, highlighting investigations into the origin and diversity of endocytic systems in eukaryotes. Beginning with a brief overview of the eukaryote tree of life, we show how understanding the endocytic machinery in a select, but diverse, array of organisms provides insights into endocytic system origins and predicts the likely configuration in the last eukaryotic common ancestor (LECA). Next, we consider three examples in which a comparative approach yielded insight into the function of modern cellular systems. First, using ESCRT-0 as an example, we show how comparative cell biology can discover both lineage-specific novelties (ESCRT-0) as well as previously ignored ancient proteins (Tom1), likely of both evolutionary and functional importance. Second, we highlight the power of comparative cell biology for discovery of previously ignored but potentially ancient complexes (AP5). Finally, using examples from ciliates and trypanosomes, we show that not all organisms possess canonical endocytic pathways, but instead likely evolved lineage-specific mechanisms. Drawing from these case studies, we conclude that a comparative approach is a powerful strategy for advancing knowledge about the general mechanisms and functions of endocytic systems.

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Evolution of Tre-2/Bub2/Cdc16 (TBC) Rab GTPase-activating proteins

Abstract: Small GTPases control many functions in cells, and the TBC GTPase-activating protein family modulates the activity of the largest G protein subfamily, Rabs. A reconstruction of the evolutionary history of TBC GAPs provides new insight into the evolution of eukaryotic cells.

Cited by 53 publications

References 56 publications

Missing Pieces of an Ancient Puzzle: Evolution of the Eukaryotic Membrane-Trafficking System

Missing Pieces of an Ancient Puzzle: Evolution of the Eukaryotic Membrane-Trafficking System

Regulation of autophagy by the Rab GTPase network

The Cell Biology of the Endocytic System from an Evolutionary Perspective

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