Evolution of the nasopharyngeal microbiota of beef cattle from weaning to 40 days after arrival at a feedlot

Timsit, Edouard; Workentine, Matthew L.; Schryvers, Anthony B.; Holman, Devin B.; Meer, Frank van der; Alexander, Trevor W.

doi:10.1016/j.vetmic.2016.03.020

Cited by 66 publications

(81 citation statements)

References 29 publications

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“…It is important to highlight that, in our study, DNS collected from the animals at the local farm were positive only for BCoV, thus the epidemiological status of the herd at arrival may be one of the reasons for the higher prevalence of this virus found in the DNS at T1. This datum supports the theory that the epidemiological status of arrival farms should be considered as a possible risk factor of BRD [4,20].…”

Section: Discussionsupporting

confidence: 86%

“…Overall, it is evident that the bovine nasopharyngeal microbiota undergoes changes following arrival at the feedlot and its evolution in beef cattle after arrival at a feedlot was also described by other researchers [4,20]. The feedlot environment, and its associated stressors, may provide conditions that allow for the proliferation of M. bovis in the nasopharynx within the first 14 days of feedlot placement [4].…”

Section: Discussionmentioning

confidence: 63%

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Prevalence of Pathogens Related to Bovine Respiratory Disease Before and After Transportation in Beef Steers: Preliminary Results

Cirone

Padalino

Tullio

et al. 2019

Animals

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Simple Summary: Bovine respiratory disease (BRD) affects the lower respiratory tract of cattle, causing high mortality. The syndrome has a multifactorial etiology and transport seems to favor pathogen proliferation. This study investigated the prevalence of different pathogens involved in BRD, in the nasal microbiota of beef steers collected before and after a long-distance journey. A total of 56 Limousine animals were included, travelling in three different shipments, on the same route from France to southern Italy in a semitrailer, on three different days from February to April. Prior to shipment (T0) and four days after arrival (T1), two deep nasopharyngeal swabs (DNS)/steer were collected and tested by bimolecular analysis. Neither bovine viral diarrhea virus (BVDV) nor bovine herpesvirus type 1 (BoHV-1) were detected. A higher prevalence of Histophilus somni was observed in the DNS collected during the third shipment in comparison with those registered during the first and the second one, probably due to a higher prevalence at departure. Conversely, the prevalence of bovine coronavirus, bovine respiratory syncytial virus, Mannheimia haemolytica, Mycoplasma bovis and Pasteurella multocida was higher on arrival in comparison with departure, confirming data reported in the literature. Overall, there were nasal microbiota changes in beef steers, with an increase in the prevalence of pathogens associated with BRD after travelling.Abstract: Bovine respiratory disease (BRD) is a serious health and economic problem in the beef industry, which is often associated with transportation and caused by different pathogens. The prevalence of bovine herpesvirus type 1 (BoHV-1), bovine adenovirus (BAdV), bovine viral diarrhea virus (BVDV), bovine coronavirus (BCoV), bovine respiratory syncytial virus (BRSV), bovine parainfluenza virus (BPiV), Pasteurella multocida, Mannheimia haemolytica, Histophilus somni, Mycoplasma bovis, in the nasal microbiota of beef steers before and after the same long-distance journey from France to southern Italy was documented. Fifty-six Limousine animals of three different shipments, travelling on three different days from February to April, were included. Prior to shipment (T0) and four days after arrival (T1), two DNS/animal were collected and tested by Real Time quantitative PCR (qPCR). Univariate logistic regression was carried out, considering time and day as fixed factors and the outcome of qPCR for each pathogen as a dependent categorical dichotomous variable (positive/negative, 1/0). The fact that the number of H. somni positive animals were found to be higher in the third shipment than the first and second one, indicating that this pathogen was already present before loading, is relevant. The prevalence of BCoV, BRSV, M. haemolytica, M. bovis, P. multocida was higher at T1 than T0, suggesting that other factors, such as stress and the epidemiological status of the arrival farm, played a role. The tested animals were not treated before and after transport, and our results are in Animals 20...

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 63%

Prevalence of Pathogens Related to Bovine Respiratory Disease Before and After Transportation in Beef Steers: Preliminary Results

Cirone

Padalino

Tullio

et al. 2019

Animals

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“…While M. haemolytica is accepted as a key pathogen in BRD (Bell et al, 2014), there is no such acceptance for M. bovirhinis. Indeed, in a recent study of the nasopharyngeal microbiota of mostly healthy cattle, Mycoplasma dispar and M. bovirhinis made up around 53% of the total bacterial population (Timsit et al, 2016). While not commonly reported, P. multocida is a recognised cause of bovine mastitis (Makovec and Ruegg, 2003).…”

Section: Discussionmentioning

confidence: 99%

A Pasteurella multocida strain affecting nulliparous heifers and calves in different ways

Turni

Dayao

Aduriz

et al. 2016

Veterinary Microbiology

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A Pasteurella multocida strain affecting nulliparous heifers and calves in different ways.Veterinary Microbiology http://dx.doi.org/10. 1016/j.vetmic.2016.08.022 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ABSTRACTPasteurella multocida isolates from dairy cattle on a farm in Spain were associated with pneumonia of calves (six isolates) and mastitis of heifers (five isolates). The objective was to determine if the P. multocida isolates retrieved from both disease scenarios were the same strain or whether more than one strain was present. The isolates were identified by a species-specific polymerase chain (PCR) assay, serotyped by the Heddleston scheme and then typed by a number of molecular genotyping assays including multi-locus sequence typing (MLST). The 11 isolates were confirmed as P. multocida but failed to react with any of the 16 Heddleston antisera. The PCR targeting the genes associated with the lipopolysaccharide outer core biosynthesis locus assigned all the isolates to L3 -the type that containsHeddleston serovars 3 and 4. The MLST analysis showed all isolates belonging to ST 79 within the clonal complex of ST13. Only one of the isolates showed a slight different profile by the repetitive extragenic palindromic PCR. The conclusion was that the same strain was associated with pneumonia in calves and mastitis in heifers.

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“…It is widely recognized that the upper respiratory tract microbiome provides a first line of defense against foreign invaders through competition, and interaction, with potential mucosal pathogens [11]. While the composition and development of the nasopharyngeal microbiota in feedlot cattle has been recently described [5, 10, 12, 13], there are no published studies describing lower respiratory tract microbial communities in feedlot cattle. Interestingly, until recently the lower respiratory tract in healthy humans has traditionally been considered as sterile using culture-dependent or conventional molecular techniques [14, 15].…”

Section: Introductionmentioning

confidence: 99%

Relationship between nasopharyngeal and bronchoalveolar microbial communities in clinically healthy feedlot cattle

et al. 2017

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BackgroundThe importance of upper airway structure in the susceptibility of the lower respiratory tract to colonization with potential pathogens is well established. With the advent of rapid, high throughput, next generation sequencing, there is a growing appreciation of the importance of commensal microbial populations in maintaining mucosal health, and a realization that bacteria colonize anatomical locations that were previously considered to be sterile. While upper respiratory tract microbial populations have been described, there are currently no published studies describing the normal microbial populations of the bovine lower respiratory tract. Consequently, we have little understanding of the relationship between upper and lower respiratory tract microbiota in healthy cattle. The primary objective of our study was to characterize the composition, structure and relationship of the lower and upper respiratory microbial communities in clinically healthy feedlot cattle. Nasopharyngeal swabs (NPS), and bronchoalveolar lavage (BAL) fluid, were collected from clinically healthy feedlot calves (n = 8). Genomic DNA from each sample was extracted, and the V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified and sequenced using Illumina Miseq platform.ResultsAcross all samples, the most predominant phyla were Proteobacteria, Actinobacteria and Firmicutes. The most common genera were Rathayibacter, Mycoplasma, Bibersteinia and Corynebacterium. The microbial community structure was distinct between these two biogeographical sites. Most of the bacterial genera identified in the BAL samples were also present in the NPS, but biogeographical-specific genera were enriched in both the NPS (Rathayibacter) and BAL (Bibersteinia) samples. There were strong associations between the presence of certain taxa at each specific location, and strong correlations between the presence of specific taxa in both the NPS and BAL samples.ConclusionsThe correlation between the presence of specific taxa in both the NPS and BAL samples, supports the notion of a mutualistic interrelationship between these microbial communities. Future studies, in large cohorts of animals, are needed to determine the role and clinical importance of the relationships of respiratory tract microbial communities with health, productivity, and susceptibility to the development of respiratory disease, in growing cattle.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-017-1042-2) contains supplementary material, which is available to authorized users.

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Evolution of the nasopharyngeal microbiota of beef cattle from weaning to 40 days after arrival at a feedlot

Cited by 66 publications

References 29 publications

Prevalence of Pathogens Related to Bovine Respiratory Disease Before and After Transportation in Beef Steers: Preliminary Results

Prevalence of Pathogens Related to Bovine Respiratory Disease Before and After Transportation in Beef Steers: Preliminary Results

A Pasteurella multocida strain affecting nulliparous heifers and calves in different ways

Relationship between nasopharyngeal and bronchoalveolar microbial communities in clinically healthy feedlot cattle

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