Evolution of Sucrose Metabolism: The Dichotomy of Invertases and Beyond

Wan, Hongjian; Wu, Limin; Yang, Yaqin; Zhou, Guozhi; Ruan, Yong‐Ling

doi:10.1016/j.tplants.2017.11.001

Cited by 181 publications

(167 citation statements)

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“…However, among the seven INV genes, only one putative cell wall invertase (CWINV, Csa3G777580 ) showed high levels of expression and distinct expression patterns in the phloem of CVB and the phloem of PlVB, while other INV genes were ubiquitously expressed at relatively low levels (Figure S8c1–c7). Recently it was reported that CWINV very likely coevolved with the development of the functional vascular system of higher plants as a component of apoplastic phloem unloading (Wan et al ., ). On the whole, it seems to imply that all the above‐mentioned genes play significant roles in RFO catabolism in different regions of the fruit.…”

Section: Resultsmentioning

confidence: 97%

“…Recently it was reported that CWINV very likely coevolved with the development of the functional vascular system of higher plants as a component of apoplastic phloem unloading (Wan et al, 2018). On the whole, it seems to imply that all the above-mentioned genes play significant roles in RFO catabolism in different regions of the fruit.…”

Section: Expression Analysis Of Rfo Metabolismmentioning

confidence: 99%

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Transcriptomic and functional analysis of cucumber (Cucumis sativus L.) fruit phloem during early development

Sui

Nie

et al. 2018

The Plant Journal

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The phloem of the Cucurbitaceae has long been a subject of interest due to its complex nature and the economic importance of the family. As in a limited number of other families, cucurbit phloem is bicollateral, i.e. with sieve tubes on both sides of the xylem. To date little is known about the specialized functions of the internal phloem (IP) and external phloem (EP). Here, a combination of microscopy, fluorescent dye transport analysis, micro-computed tomography, laser capture microdissection and RNA-sequencing (RNA-Seq) were used to study the functions of IP and EP in the vascular bundles (VBs) of cucumber fruit. There is one type of VB in the peduncle, but four in the fruit: peripheral (PeVB), main (MVB), carpel (CVB) and placental (PlVB). The VBs are bicollateral, except for the CVB and PlVB. Phloem mobile tracers and C applied to leaves are transported primarily in the EP, and to a lesser extent in the IP. RNA-Seq data indicate preferential gene transcription in the IP related to differentiation/development, hormone transport, RNA or protein modification/processing/transport, and nitrogen compound metabolism and transport. The EP preferentially expresses genes for stimulus/stress, defense, ion transport and secondary metabolite biosynthesis. The MVB phloem is preferentially involved in photoassimilate transport, unloading and long-distance signaling, while the PeVB plays a more substantial role in morphogenesis and/or development and defense response. CVB and PlVB transcripts are biased toward development of reproductive organs. These findings provide an integrated view of the differentiated structure and function of the vascular tissue in cucumber fruit.

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Section: Resultsmentioning

confidence: 97%

Section: Expression Analysis Of Rfo Metabolismmentioning

confidence: 99%

Transcriptomic and functional analysis of cucumber (Cucumis sativus L.) fruit phloem during early development

Sui

Nie

et al. 2018

The Plant Journal

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“…Japonica (Os‐1, NP _001049936; Os‐2, XP _015625957.1; Os‐3, XP _015627031.1), Pleurocapsa sp. PCC 7319 (Pl‐1, WP _019507642.1), Prochlorococcus marinus (Pm‐1, WP _011131014.1; Pm‐2, WP _036892687.1), Physcomitrella patens (Pp‐1, XP _001754878.1; Pp‐2, XP _001758344.1) Stanieria cyanosphaera (Sc‐1, WP _041619725.1; Sc‐2, WP _015195523.1), Selaginella moellendorffii (Sm‐1, XP _002978791.1; Sm‐2, XP _002968256.1), Spirogyra pratensis (Sp‐1, GBSM 01000698.1; Sp‐2, GBSM 01023190.1; obtained from the Transcriptome Shotgun Assembly database of NCBI and referred to reference ), Synechococcus sp. WH 8020 (Sy‐8020‐1, WP _048348470.1), Synechococcus sp.…”

Section: Discussionmentioning

confidence: 99%

Structural and enzymatic analyses of Anabaena heterocyst‐specific alkaline invertase InvB

Xie

Cai

et al. 2018

FEBS Letters

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Anabaena sp. PCC 7120 encodes two alkaline/neutral invertases, namely InvA and InvB. Following our recently reported InvA structure, here we report the crystal structure of the heterocyst-specific InvB. Despite sharing an overall structure similar to InvA, InvB possesses a much higher catalytic activity. Structural comparisons of the catalytic pockets reveal that Arg430 of InvB adopts a different conformation, which may facilitate the deprotonation of the catalytic residue Glu415. We propose that the higher activity may be responsible for the vital role of InvB in heterocyst development and nitrogen fixation. Furthermore, phylogenetic analysis combined with activity assays also suggests the role of this highly conserved arginine in plants and cyanobacteria, as well as some proteobacteria living in highly extreme environments.

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“…It has also been found that the FINS1/FBP (fructose insensitive1 and putative fructose-1,6-bisphosphatase) protein plays a key role in the signaling pathway induced by fructose [38,83]. Some researchers have suggested the involvement of invertases (EC 3.2.1.26 -β-fructofuranosidase) in the reception of sugar signal [9,89,90]. The participation of invertase could be direct, in which case the signal would be passed directly to cascades of specific kinases.…”

Section: Receiving and Transmitting The Signals Triggered By Sugarsmentioning

confidence: 99%

Regulatory roles of sugars in plant growth and development

Ciereszko

2018

Acta Soc Bot Pol

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In recent years, several studies have focused on the factors and mechanisms that regulate plant growth and development, as well as the functioning of signaling pathways in plant cells, unraveling the involvement of sugars in the processes regulating such growth and development. Saccharides play an important role in the life of plants: they are structural and storage substances, respiratory substrates, and intermediate metabolites of many biochemical processes. Sucrose is the major transport form of assimilates in plants. Sugars can also play an important role in the defense reactions of plants. However, it has been shown that glucose, sucrose, or trehalose-6-phosphate (Tre6P) can regulate a number of growth and metabolic processes, acting independently of the basal functions; they can also act as signaling molecules. Changes in the concentration, qualitative composition, and transport of sugars occur continuously in plant tissues, during the day and night, as well as during subsequent developmental stages. Plants have developed an efficient system of perception and transmission of signals induced by lower or higher sugar availability. Changes in their concentration affect cell division, germination, vegetative growth, flowering, and aging processes, often independently of the metabolic functions. Currently, the mechanisms of growth regulation in plants, dependent on the access to sugars, are being increasingly recognized. The plant growth stimulating system includes hexokinase (as a glucose sensor), trehalose-6-phosphate, and TOR protein kinase; the lack of Tre6P or TOR kinase inhibits the growth of plants and their transition to the generative phase. It is believed that the plant growth inhibition system consists of SnRK1 protein kinases and C/S1 bZIP transcription factors. The signal transduction routes induced by sugars interact with other pathways in plant tissues (for example, hormonal pathways) creating a complex communication and signaling network in plants that precisely controls plant growth and development.

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Evolution of Sucrose Metabolism: The Dichotomy of Invertases and Beyond

Cited by 181 publications

References 63 publications

Transcriptomic and functional analysis of cucumber (Cucumis sativus L.) fruit phloem during early development

Transcriptomic and functional analysis of cucumber (Cucumis sativus L.) fruit phloem during early development

Structural and enzymatic analyses of Anabaena heterocyst‐specific alkaline invertase InvB

Regulatory roles of sugars in plant growth and development

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