Evolution of soil biological properties after addition of glyphosate, diflufenican and glyphosate+diflufenican herbicides

Application of Diflufenican Herbicide on Soils Amended with Different Organic Wastes

“…These results are in agreement with those obtained by Tejada (2009), which observed the toxic effect of this herbicide in microcosm studies. Therefore, the study of soil enzymatic activities are an essential tool for knowing the state of soil pollution (Gianfreda et al, 2005;Tejada et al, 2007, Tejada, 2009 Column followed by the same letter(s) are not significantly different (p<0.05) was higher the clay loam texture soil than in sandy loam texture soil, the inhibition of soil enzymatic activities in soil was higher in the Calciorthid Xerollic soil than in the Typic Xerofluvent soil. The application of organic matter in unpolluted soils significantly increased enzymatic activities (Ros et al, 2003;Ferreras et al, 2006, Tejada et al, 2010.…”

“…Organic wastes were mechanically mixed with the soil, allowing the organic wastes to reach up to 25 cm from the soil surface At 3,15,45,90,120,150,180,210 and 250 days after applying the herbicide to soil, diflufenican was measured. To extract diflufenican we used the method described by Tejada (2009). Five grams of soil was shaken with 20 ml of 90:10 v/v methanol:H 2 O for 1 h at 25 ± 1º C. After allowing the soil to settle, pesticide concentrations were determined by HPLC using a column Merck C18 ultrasphere (250 x 4.6 mm x 5 µm and 80 Å).…”

Response of soil phosphatases to glyphosate and its formulations - Roundup (laboratory conditions)

“…The effect of glyphosate on soil enzymes involved in phosphorus metabolism gave conflicting results: inhibition (Sannino and Gianfreda 2001, Floch et al 2011, Yu et al 2011 and stimulation (Nakatani et al 2014, Cherni et al 2015. Tejada (2009) reported that the effect of glyphosate on phosphatases is higher in sandy soil than in clay soil. The results are in agreement with our previous study (Płatkowski and Telesiński 2015b).…”

Pseudomonas putida and Bacillus amyloliquefaciens alleviates the adverse effect of pesticides and poise soil enzymes activities in chickpea (Cicer arietinum L.) rhizosphere

“…On the application of herbicides the activity of acid and alkaline phosphatase is either stimulated (imazethapyr) or unchanged (aurora 40 WG and rimsulfuron) (Perucci et al 2000, Baćmaga et al 2012. Phosphatases activities were found to be inhibited by herbicide application under different conditions of soil physicochemical properties and pesticides dose (Min et al 2001, Tejada 2009). The acid and alkaline phosphatase activities may respond differently to application of insecticides.…”