Evolution of MNC and lymphocyte collection settings employing different Spectra Optia^® Leukapheresis systems

Abstract

The Spectra Optia CMNC device using lower centrifugal force (PF4·0) showed similar target cell yield and composition as well as collection efficiencies with superior performance parameters and lower PLT contamination compared to the MNC setting.

Low-Volume Leukapheresis in Non-Cytokine-Stimulated Donors for the Collection of Mononuclear Cells

“…But in contrast to our data, the perfect correlation of both variables could not be shown. Regarding the RF values, the values of Punzel et al [10] for WBCs, MNCs, and lymphocytes were nearly twice as high when compared to our values. The same applies to the RF values for CD45+, CD3+ and CD14+ cells presented by Strasser et al 2005 [15] and 2007 [18].…”

“…In correspondence with these results, Svensson et al [17] found a strong correlation between the concentrations of monocytes and lymphocytes in the blood pre-apheresis and the number of monocytes and lymphocytes collected. In contrast, Punzel et al [10] detected only a weak correlation between the CD3+ pre-apheresis values and the CD3+ cell yield in the product. Steininger et al [11] could show a significant correlation between the pre-apheresis cell count and the correspondent product cell yield for CD14+ but not for CD3+ cells.…”

“…All leukapheresis procedures were performed via peripheral vein access using the CMNC program (software version 11.3) of the Spectra Optia system (Terumo BCT) according to the instructions of the manufacturer and default settings described previously [10]. Unlike Punzel et al [10] the packing factor was unaltered and used at its default setting of 4.5.…”

“…Unlike Punzel et al [10] the packing factor was unaltered and used at its default setting of 4.5. For anticoagulation the acid-citrate-dextrose formula A (ACD-A) (Terumo BCT) was used without additional heparin.…”

“…However, there is hardly any published data about MNC collection with this program in non-stimulated healthy donors. The first study which compared the earlier introduced discontinuous MNC program on the Spectra Optia to the CMNC program for MNC collection indicated a higher efficiency of the CMNC program, resulting in a smaller product volume and a shorter procedure time by comparable MNC yield [10]. Notwithstanding the above, the CMNC program remains easier to monitor and can be controlled more precisely than the MNC program.…”