Evolution of resistance to cationic biocides in Streptococcus mutans and Enterococcus faecalis

Kitagawa, Haruaki; Izutani, Naomi; Kitagawa, Ranna; Maezono, Hazuki; Yamaguchi, Manabu; Imazato, Satoshi

doi:10.1016/j.jdent.2016.02.008

Cited by 74 publications

(84 citation statements)

References 33 publications

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“…Regarding QAC-based biomedical materials, there is an urgent need to evaluate whether the long-term use of such technologies results in reduced microbial susceptibility to the QAC and the emergence of QAC-resistant strains. A recent study investigated whether S. mutans and E. faecalis could develop resistance to the cationic biocides [409]. There were no changes in the minimum inhibitory concentration of MDPB to S. mutans and E. faecalis even after ten passages, suggesting that no antimicrobial resistance has been developed.…”

Section: Qacs and Antimicrobial Resistancementioning

confidence: 99%

Quaternary ammonium-based biomedical materials: State-of-the-art, toxicological aspects and antimicrobial resistance

Jiao

Niu

et al. 2017

Progress in Polymer Science

483

317

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a b s t r a c tMicrobial infections affect humans worldwide. Many quaternary ammonium compounds have been synthesized that are not only antibacterial, but also possess antifungal, antiviral and anti-matrix metalloproteinase capabilities. Incorporation of quaternary ammonium moieties into polymers represents one of the most promising strategies for preparation of antimicrobial biomaterials. Various polymerization techniques have been employed to prepare antimicrobial surfaces with quaternary ammonium functionalities; in particular, syntheses involving controlled radical polymerization techniques enable precise control over macromolecular structure, order and functionality. Although recent publications report exciting advances in the biomedical field, some of these technological developments have also been accompanied by potential toxicological and antimicrobial resistance challenges. Recent evidenced-based data on the biomedical applications of antimicrobial quaternary ammonium-containing biomaterials that are based on randomized human clinical trials, the golden standard in contemporary medicinal science, are included in the present review. This should help increase visibility, stimulate debates and spur conversations within a wider scientific community on the implications and plausibility for future developments of quaternary ammonium-based antimicrobial biomaterials.Published by Elsevier B.V.

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Section: Qacs and Antimicrobial Resistancementioning

confidence: 99%

Quaternary ammonium-based biomedical materials: State-of-the-art, toxicological aspects and antimicrobial resistance

Jiao

Niu

et al. 2017

Progress in Polymer Science

483

317

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“…Accordingly, Kitagawa et al. () recently reported development of enhanced tolerance toward CHX in Enterococcus faecalis as shown by increased minimum inhibitory concentrations (MICs) after 10 passages of treatment and regrowth. Likewise, Wang et al.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, it has been recommended to limit the use of CHX to "those applications with a clear patient benefit" (i.e., intensive care) in order to reduce the risk of inducing acquired resistance toward CHX or even cross-resistances toward antibiotics (Kampf, 2016). Accordingly, Kitagawa et al (2016) recently reported development of enhanced tolerance toward CHX in Enterococcus faecalis as shown by increased minimum inhibitory concentrations (MICs) after 10 passages of treatment and regrowth. Likewise, found increased MICs in E. faecalis, Streptococcus gordonii, Fusobacterium nucleatum, and Porphyromonas gingivalis after 10 suchlike passages.…”

mentioning

confidence: 99%

Antimicrobial efficacy of alternative compounds for use in oral care toward biofilms from caries‐associated bacteria in vitro

Kara¹,

Muehler²,

Enax

et al. 2018

MicrobiologyOpen

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For caries‐active patients, antimicrobial measures may be useful in addition to mechanical biofilm removal. The aim of this study was to investigate the antimicrobial efficacy of alternative compounds for use in oral care from two main categories (i.e., preservatives and natural compounds) toward biofilms from caries‐associated bacteria as compared to oral care gold‐standards chlorhexidine digluconate ( CHX ), cetylpyridinium chloride ( CPC ), and zinc. Compounds were screened in initial Streptococcus mutans biofilms. Then, the most effective compounds were further investigated in mature S. mutans and polymicrobial biofilms comprising Actinomyces naeslundii , Actinomyces odontolyticus, and S. mutans . Here, distinct treatment periods and concentrations were evaluated. Biofilms were visualized by scanning electron microscopy and bacterial membrane damage was evaluated by means of flow cytometry and staining with SYBR Green and propidium iodide. Citrus extract was the only compound exhibiting similar antimicrobial efficacy in initial S. mutans biofilms (>5 log 10 ) as compared to CHX and CPC , but its effect was clearly inferior in mature S. mutans and polymicrobial biofilms. Flow cytometric data suggested that the mechanism of antimicrobial action of citrus extract may be based on damage of bacterial membranes similar to CHX and CPC . From all alternative compounds investigated in this study, citrus extract exhibited the highest antimicrobial efficacy toward in vitro biofilms from caries‐associated bacteria, but still was less effective than oral care gold‐standard antiseptics CHX and CPC . Nevertheless, citrus extract may be a valuable antimicrobial compound for use in oral care for caries‐active patients.

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“…Since then, the benefit of NaOCl against bacteria on dentin surfaces has also been demonstrated [15][16][17] . QACs, such as cetylpyridinium chloride or benzalkonium chloride, are widely used as antibacterial agents 18) . QACs are proposed to interact with negatively charged bacterial cell surfaces, causing a disturbance to the charge balance and damage to the cell membrane 5) .…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity and dentin bonding ability of combined use of Clearfil SE Protect and sodium hypochlorite

et al. 2018

Self Cite

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The aim of this study was to evaluate the antibacterial activity and dentin bonding ability of a commercial self-etch adhesive Clearfil SE Protect (Kuraray Noritake Dental, Tokyo, Japan) in combination with sodium hypochlorite (NaOCl). Agar disc diffusion tests and measurement of minimum inhibitory/bactericidal concentrations (MIC/MBC) against Streptococcus mutans were performed to evaluate antibacterial effects. The mixture solution of 5.25% NaOCl and the primer of Clearfil SE Protect demonstrated less antibacterial activity than primer only. In microtensile bond strength tests using non-carious human molars, pretreatment with 5.25% NaOCl aqueous solution had no influence on the bond strength of Clearfil SE Protect. These results indicate that pretreatment with NaOCl does not influence the bonding ability of Clearfil SE Protect, while their combined use does not enhance cavity disinfecting effects.

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Evolution of resistance to cationic biocides in Streptococcus mutans and Enterococcus faecalis

Cited by 74 publications

References 33 publications

Quaternary ammonium-based biomedical materials: State-of-the-art, toxicological aspects and antimicrobial resistance

Quaternary ammonium-based biomedical materials: State-of-the-art, toxicological aspects and antimicrobial resistance

Antimicrobial efficacy of alternative compounds for use in oral care toward biofilms from caries‐associated bacteria in vitro

Antibacterial activity and dentin bonding ability of combined use of Clearfil SE Protect and sodium hypochlorite

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