Evolution of Plant Homeobox Genes

Mukherjee, Krishanu; Brocchieri, Luciano

doi:10.1002/9780470015902.a0022865

Cited by 4 publications

(3 citation statements)

References 56 publications

(81 reference statements)

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“…However, despite our efforts, the role of ZmHB87 and ZmCPP8 on the regulation of ZmPEPC1 gene expression is still not clear and requires further studies. ZmHB87 is a member of the homeobox TF family reported to form homoand heter-odimers (Meijer et al, 2000;Mukherjee and Brocchieri, 2010). Nevertheless, under our experimental conditions, ZmHB87 did not form homodimers, did not interact with the other TFs analyzed, and did not show any trans-activation activity on the ZmPEPC1 upstream region.…”

Section: Discussionmentioning

confidence: 58%

ZmOrphan94 Transcription Factor Downregulates ZmPEPC1 Gene Expression in Maize Bundle Sheath Cells

et al. 2021

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Spatial separation of the photosynthetic reactions is a key feature of C4 metabolism. In most C4 plants, this separation requires compartmentation of photosynthetic enzymes between mesophyll (M) and bundle sheath (BS) cells. The upstream region of the gene encoding the maize PHOSPHOENOLPYRUVATE CARBOXYLASE 1 (ZmPEPC1) has been shown sufficient to drive M-specific ZmPEPC1 gene expression. Although this region has been well characterized, to date, only few trans-factors involved in the ZmPEPC1 gene regulation were identified. Here, using a yeast one-hybrid approach, we have identified three novel maize transcription factors ZmHB87, ZmCPP8, and ZmOrphan94 as binding to the ZmPEPC1 upstream region. Bimolecular fluorescence complementation assays in maize M protoplasts unveiled that ZmOrphan94 forms homodimers and interacts with ZmCPP8 and with two other ZmPEPC1 regulators previously reported, ZmbHLH80 and ZmbHLH90. Trans-activation assays in maize M protoplasts unveiled that ZmHB87 does not have a clear transcriptional activity, whereas ZmCPP8 and ZmOrphan94 act as activator and repressor, respectively. Moreover, we observed that ZmOrphan94 reduces the trans-activation activity of both activators ZmCPP8 and ZmbHLH90. Using the electromobility shift assay, we showed that ZmOrphan94 binds to several cis-elements present in the ZmPEPC1 upstream region and one of these cis-elements overlaps with the ZmbHLH90 binding site. Gene expression analysis revealed that ZmOrphan94 is preferentially expressed in the BS cells, suggesting that ZmOrphan94 is part of a transcriptional regulatory network downregulating ZmPEPC1 transcript level in the BS cells. Based on both this and our previous work, we propose a model underpinning the importance of a regulatory mechanism within BS cells that contributes to the M-specific ZmPEPC1 gene expression.

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Section: Discussionmentioning

confidence: 58%

ZmOrphan94 Transcription Factor Downregulates ZmPEPC1 Gene Expression in Maize Bundle Sheath Cells

et al. 2021

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“…This DNA-binding domain is composed of three helices of which the second and third helices adopt a helix-turn-helix motif involved in DNA recognition and binding (Mukherjee and Brocchieri, 2010).…”

Section: Resultsmentioning

confidence: 99%

Molecular cloning and expression analysis of zinc finger-homeodomain transcription factor TaZFHD1 in wheat

Abu-Romman

2014

South African Journal of Botany

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A zinc finger-homeodomain (ZFHD) gene was isolated from Triticum aestivum using in silico cloning and reverse transcription (RT-PCR) approaches. The full length cDNA of T. aestivum, designed as TaZFHD1 (GenBank accession No. KF697362), was 1345 bp long. The open reading frame (ORF) of TaZFHD1 was 1164 bp and was predicted to encode 387 amino-acid protein that contained putative zinc finger domain in the N-terminus and a DNA binding homeodomain in the C-terminus. Analysis of the deduced amino acid sequence of TaZFHD1 revealed that it had high homology to other plants ZFHD proteins. The expression of TaZFHD1 was analyzed during spike development and after exposure to different hormonal stimuli using semi-quantitative RT-PCR analysis. TaZFHD1 was differentially expressed during spike development, with a preferential expression during 'half emerged', 'completely emerged', and 'half anthesis' stages. Methyl jasmonate (MeJA), abcissic acid (ABA) and ethylene (ET) upregulated the expression of TaZFHD1. However, there was no remarkable difference in TaZFHD1 expression after treatment with salicylic acid (SA). These results might indicate that TaZFHD1 may be involved in wheat anthesis and pollination and in JA-, ABA-, and ET-mediated signaling pathways.

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“…Homeobox domain is present in 14 classes of transcription factors in plants. They are involved in wide spectrum of processes (signal transduction networks involved in response to abscisic acid, auxin and drought, general growth regulation, in shoot and floral meristem development, stem-cell specification and proliferation, flowering time regulation directly repress gibberellin and lignin biosynthetic gene expression and in the adaptation to drought and disease resistance to fungal pathogens and many more (138). More recently Khan et al (139) investigated involvement of homeobox genes in Brassica rapa under various stress conditions.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptomics of sevenImpatiensspecies

Šurinová

Stočes²,

Dostálek

et al. 2021

Preprint

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Impatiens is a genus containing more than 1000 species. Thanks to its size, it is a unique system for studying species diversification in natural populations. This study focused on the characterization of novel transcriptomes from seven Impatiens species originating from Nepal. Leave transcriptome of Impatines balsamina L. , I. racemosa DC. , I. bicornuta Wall , I. falcifer Hook , I. devendrae Pusalkar, I. scullyi Hook and I. scabrida DC were sequenced and compared. Reads were de novo assembled and aligned to 92 035-226 081 contigs. We identified 14 728 orthology groups shared among all the species and 3 020 which were unique to a single species. In single species, 2536-3009 orthology groups were under selection from which 767 were common for all species. Six of the seven investigated species shared 77% of gene families with I. bicornuta being the most distinct species. Specific gene families involved in response to different environmental stimuli were closely described. Impatiens bicornuta selection profile shared selection on zing finger protein structures and flowering regulation and stress response proteins with the other investigated species. Overall, the study showed substantial similarity in patterns of selections on transcribed genes across the species suggesting similar evolutionary pressures. This suggests that the species group may have evolved via adaptive radiation.

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Evolution of Plant Homeobox Genes

Cited by 4 publications

References 56 publications

ZmOrphan94 Transcription Factor Downregulates ZmPEPC1 Gene Expression in Maize Bundle Sheath Cells

ZmOrphan94 Transcription Factor Downregulates ZmPEPC1 Gene Expression in Maize Bundle Sheath Cells

Molecular cloning and expression analysis of zinc finger-homeodomain transcription factor TaZFHD1 in wheat

Comparative transcriptomics of sevenImpatiensspecies

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