Evolution of mobile genetic element composition in an epidemic methicillin-resistant Staphylococcus aureus: temporal changes correlated with frequent loss and gain events

Jamrozy, Dorota; Coll, Francesc Español i; Mather, Alison E.; Harris, Simon R.; Harrison, Ewan M.; MacGowan, Alasdair; Karas, Andreas; Elston, T.; Török, M. Estée; Parkhill, Julian; Peacock, Sharon J.

doi:10.1186/s12864-017-4065-z

Cited by 41 publications

(40 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These procedures were performed using the binaryPGLMM function of the R package “rr2” and the phyloglm function of the R package “phylolm” (Ho & Ané, 2014; Ives & Garland, 2010, 2014; Ives, Helmus, & Ves, 2011; Paradis, Claude, & Strimmer, 2004). In the binaryPGLMM function, s 2 is the scaling component of the variance in the PGLMM, where s 2 = 0 suggests no phylogenetic signal and a high s 2 value implies strong phylogenetic signal (Jamrozy et al., 2017). In the phyloglm function, alpha is the phylogenetic correlation parameter (an alpha value close to 0 suggests strong phylogenetic signal, alpha = 1 indicates a phylogenetic signal of trait evolution consistent with the expectation under Brownian motion, and an alpha value close to infinity implies low phylogenetic signal) (Blumstein et al., 2015; Gallien et al., 2016; O'Meara, Graham, Pellis, & Burghardt, 2015).…”

Section: Methodsmentioning

confidence: 99%

Are dominant plant species more susceptible to leaf‐mining insects? A case study at Saihanwula Nature Reserve, China

Dai

Long

et al. 2018

Ecology and Evolution

View full text Add to dashboard Cite

Dominant species significantly affect interspecific relationships, community structure, and ecosystem function. In the field, dominant species are often identified by their high importance values. Selective foraging on dominant species is a common phenomenon in ecology. Our hypothesis is that dominant plant groups with high importance values are more susceptible to leaf‐mining insects at the regional level. Here, we used the Saihanwula National Nature Reserve as a case study to examine the presence–absence patterns of leaf‐mining insects on different plants in a forest‐grassland ecotone in Northeast China. We identified the following patterns: (1) After phylogenetic correction, plants with high importance values are more likely to host leafminers at the species, genus, or family level. (2) Other factors including phylogenetic isolation, life form, water ecotype, and phytogeographical type of plants have different influences on the relationship between plant dominance and leafminer presence. In summary, the importance value is a valid predictor of the presence of consumers, even when we consider the effects of plant phylogeny and other plant attributes. Dominant plant groups are large and susceptible targets of leaf‐mining insects. The consistent leaf‐mining distribution pattern across different countries, vegetation types, and plant taxa can be explained by the “species‐area relationship” or the “plant apparency hypothesis.”

show abstract

Section: Methodsmentioning

confidence: 99%

Are dominant plant species more susceptible to leaf‐mining insects? A case study at Saihanwula Nature Reserve, China

Dai

Long

et al. 2018

Ecology and Evolution

View full text Add to dashboard Cite

show abstract

“…The copB and mco genes were carried by all isolates belonging to CC30, by 50% of isolates from CC22 and by a single CC8 isolate. The plasmid carrying the copB-mco operon was also recently reported to be carried by 43-70% of bloodstream infection isolates of S. aureus (mostly CC22) from the UK and Ireland sampled between 2001 and 2010 (3). There is evidence of extensive loss and gain of the pSCBU (p2-hm) plasmid (3) highlighting the mobility of the copB-mco locus within populations of S. aureus .…”

Section: Discussionmentioning

confidence: 86%

“…The copB - mco copper-hypertolerance locus is carried either on a replicating plasmid or on a plasmid integrated into the chromosome (2, 3, 5). The role of MGE-encoded copper resistance genes in MRSA was studied using the copB - mco locus-carrying plasmid pSCBU, (3). Plasmid pSCBU, also known as P2-hm, was previously found to be carried by a population of MRSA CC22 bloodstream isolates from the UK and Ireland (3).…”

Section: Resultsmentioning

confidence: 99%

“…The role of MGE-encoded copper resistance genes in MRSA was studied using the copB - mco locus-carrying plasmid pSCBU, (3). Plasmid pSCBU, also known as P2-hm, was previously found to be carried by a population of MRSA CC22 bloodstream isolates from the UK and Ireland (3). For the purposes of this study, pSCBU was introduced into CC22 strain 14-2533T (Table S1).…”

Section: Resultsmentioning

confidence: 99%

“…A copper-hypertolerance locus ( copB - mco ) has been described in some clinically relevant strains of S. aureus , carried either on a replicating plasmid or on a plasmid integrated into the chromosome (Fig. 1A) (2, 3, 5). The copB gene encodes a second copper exporting P1-type ATPase (CopB) and mco encodes a multicopper oxidase implicated in copper homeostasis and the oxidative stress response (23).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mobile genetic element-encoded hypertolerance to copper protectsStaphylococcus aureusfrom killing by host phagocytes

Zapotoczna

Pelicioli-Riboldi

Moustafa

et al. 2018

Preprint

View full text Add to dashboard Cite

22Pathogens are exposed to toxic levels of copper during infection and copper tolerance 23 may be a general virulence mechanism used by bacteria to resist host defences. In support of 24 this, inactivation of copper-exporter genes has been found to reduce the virulence of bacterial 25 pathogens in vivo. Here we investigate the role of copper-hypertolerance in methicillin 26 resistant Staphylococcus aureus. We show that a copper-hypertolerance locus (copB-mco), 27 carried on a mobile genetic element, is prevalent in a collection of invasive S. aureus strains 28 and more widely among clonal complex 22, 30 and 398 strains. The copB and mco genes 29 encode a copper efflux pump and a multicopper oxidase, respectively. Isogenic mutants 30 lacking copB or mco had impaired growth in subinhibitory concentrations of copper. Transfer 31 of a copB-mco encoding plasmid to a naive clinical isolate resulted in a gain of copper-32 hypertolerance and enhanced bacterial survival inside primed macrophages. The copB and 33 mco genes were upregulated within infected macrophages and their expression was 34 dependent on the copper sensitive operon repressor CsoR. Isogenic copB and mco mutants 35were impaired in their ability to persist intracellularly in macrophages and were less resistant 36 to phagocytic killing in human blood than the parent strain. The importance of copper-37 regulated genes in resistance to phagocytic killing was further elaborated using mutants 38 expressing a copper-insensitive variant of CsoR. Our findings suggest that the gain of mobile 39 genetic elements carrying copper-hypertolerance genes contributes to the evolution of 40 virulent strains of S. aureus, better equipped to resist killing by host immune cells. 41 42 43

show abstract

Acquired Resistance from Gene Transfer

Grohmann

Kohler

Vaishampayan

2019

Antibiotic Drug Resistance

View full text Add to dashboard Cite

Evolution of mobile genetic element composition in an epidemic methicillin-resistant Staphylococcus aureus: temporal changes correlated with frequent loss and gain events

Cited by 41 publications

References 67 publications

Are dominant plant species more susceptible to leaf‐mining insects? A case study at Saihanwula Nature Reserve, China

Are dominant plant species more susceptible to leaf‐mining insects? A case study at Saihanwula Nature Reserve, China

Mobile genetic element-encoded hypertolerance to copper protectsStaphylococcus aureusfrom killing by host phagocytes

Acquired Resistance from Gene Transfer

Contact Info

Product

Resources

About