Evolution of IncA/C
 bla
 CMY-2
 -Carrying Plasmids by Acquisition of the
 bla
 NDM-1
 Carbapenemase Gene

Carattoli, Alessandra; Villa, Laura; Poirel, Laurent; Bonnin, Rémy A.; Nordmann, Patrice

doi:10.1128/aac.05116-11

Cited by 133 publications

(92 citation statements)

References 36 publications

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“…Similar to the aforementioned IncN2 bla NDM-1 genetic contexts, the IncA/C bla NDM-1 genetic context was also observed within two different species within the same patient (patient 4). Further, it is very similar to the most frequently reported IncA/C bla NDM-1 genetic context of the Enterobacteriaceae family (8,14,31). The combination of IncA/C bla NDM-1 genetic context identification (Fig.…”

Section: Figsupporting

confidence: 54%

Genetic Contexts of bla _NDM-1 in Patients Carrying Multiple NDM-Producing Strains

Wailan

Sartor

Zowawi

et al. 2015

Antimicrob Agents Chemother

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hThe carbapenem resistance determinant bla NDM-1 has been found in various Gram-negative bacteria and upon different plasmid replicon types (Inc). Here, we present four patients within two hospitals in Pakistan harboring between two and four NDM-1-producing Gram-negative bacilli of different species coresident in their stool samples. We characterize the bla NDM-1 genetic contexts of these 11 NDM-1-producing Gram-negative bacilli in addition to other antimicrobial resistance mechanisms, plasmid replicon profiles, and sequence types (STs) in order to understand the underlying acquisition mechanisms of carbapenem resistance within these bacteria. Two common plasmid types (IncN2 and IncA/C) were identified to carry bla NDM-1 among the six different bacterial species isolated from the four patients. Two of these strains were novel Citrobacter freundii ST 20 and ST 21. The same IncN2-type bla NDM-1 genetic context was found in all four patients and within four different species. The IncA/C-type bla NDM-1 genetic context was found in two different species and in two of the four patients. Combining genetic context characterization with other molecular epidemiology methods, we were able to establish the molecular epidemiological links between genetically unrelated bacterial species by linking their acquisition of an IncN2 or IncA/C plasmid carrying bla NDM-1 for carbapenem resistance. By combining plasmid characterization and in-depth genetic context assessment, this analysis highlights the importance of plasmids in antimicrobial resistance. It also provides a novel approach for investigating the underlying mechanisms of bla NDM-1 -related spread between bacterial species and genera via plasmids.

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Section: Figsupporting

confidence: 54%

Genetic Contexts of bla _NDM-1 in Patients Carrying Multiple NDM-Producing Strains

Wailan

Sartor

Zowawi

et al. 2015

Antimicrob Agents Chemother

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“…Molecular epidemiological studies indicated that the IncA/Ctype broad-host-range plasmids associated with mosaic genetic structures (e.g., ISAba1) are mainly responsible for spreading bla NDM-1 among the Enterobacteriaceae (4,33). Moreover, the IncFII, IncN, IncH, and IncL/M types of plasmids have also been associated with bla NDM (34)(35)(36).…”

Section: Resultsmentioning

confidence: 99%

Colistin-Nonsusceptible Pseudomonas aeruginosa Sequence Type 654 with bla _NDM-1 Arrives in North America

Mataseje¹,

Peirano

Church

et al. 2016

Antimicrob Agents Chemother

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gThis study describes 3 different bla NDM-1 genetic platforms in 3 different species obtained from the same patient who was directly transferred to an institution in Calgary, Alberta, Canada, following a prolonged hospital stay in India. The bla NDM-1 in the Escherichia coli isolate was located on a 176-kb IncA/C plasmid contained within an ISCR1 region. The bla NDM-1 in the Providencia rettgeri isolate was located on a 117-kb IncT plasmid contained within Tn3000, while the bla NDM-1 in the Pseudomonas aeruginosa isolate was located on the chromosome within an ISCR3 region. This report highlights the plasticity of the genetic regions and environments associated with bla NDM-1 . To the best of our knowledge, this is the first report of P. aeruginosa with bla NDM-1 identified in North America and the first report of bla OXA-181 in P. rettgeri. The P. aeruginosa isolate belonged to the international high-risk sequence type 654 clone and was nonsusceptible to colistin. This case emphasizes the need for the use of appropriate infection prevention and control measures and vigilant screening for carbapenemresistant Gram-negative bacteria in patients with a history of travel to areas of endemicity, such as the Indian subcontinent.

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“…The bla NDM-1 gene was originally identified among Klebsiella pneumoniae isolates from infections originating in India. However, it was quickly realized that this gene was plasmid borne, was present on multiple plasmid types ( [164][165][166][167], and was rapidly spreading between bacteria (168). The first report of bla NDM in Salmonella involved a patient with a Klebsiella pneumoniae infection that was resistant to carbapenems (169).…”

Section: Role Of Plasmids In Salmonella Genome Evolutionmentioning

confidence: 99%

Salmonella Pathogenicity and Host Adaptation in Chicken-Associated Serovars

Foley

Johnson

Ricke

et al. 2013

Microbiol Mol Biol Rev

263

218

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SUMMARY Enteric pathogens such as Salmonella enterica cause significant morbidity and mortality. S. enterica serovars are a diverse group of pathogens that have evolved to survive in a wide range of environments and across multiple hosts. S. enterica serovars such as S . Typhi, S . Dublin, and S . Gallinarum have a restricted host range, in which they are typically associated with one or a few host species, while S . Enteritidis and S . Typhimurium have broad host ranges. This review examines how S. enterica has evolved through adaptation to different host environments, especially as related to the chicken host, and continues to be an important human pathogen. Several factors impact host range, and these include the acquisition of genes via horizontal gene transfer with plasmids, transposons, and phages, which can potentially expand host range, and the loss of genes or their function, which would reduce the range of hosts that the organism can infect. S . Gallinarum, with a limited host range, has a large number of pseudogenes in its genome compared to broader-host-range serovars. S. enterica serovars such as S . Kentucky and S . Heidelberg also often have plasmids that may help them colonize poultry more efficiently. The ability to colonize different hosts also involves interactions with the host's immune system and commensal organisms that are present. Thus, the factors that impact the ability of Salmonella to colonize a particular host species, such as chickens, are complex and multifactorial, involving the host, the pathogen, and extrinsic pressures. It is the interplay of these factors which leads to the differences in host ranges that we observe today.

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Evolution of IncA/C bla _CMY-2 -Carrying Plasmids by Acquisition of the bla _NDM-1 Carbapenemase Gene

Cited by 133 publications

References 36 publications

Genetic Contexts of bla _NDM-1 in Patients Carrying Multiple NDM-Producing Strains

Genetic Contexts of bla _NDM-1 in Patients Carrying Multiple NDM-Producing Strains

Colistin-Nonsusceptible Pseudomonas aeruginosa Sequence Type 654 with bla _NDM-1 Arrives in North America

Salmonella Pathogenicity and Host Adaptation in Chicken-Associated Serovars

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Evolution of IncA/C bla CMY-2 -Carrying Plasmids by Acquisition of the bla NDM-1 Carbapenemase Gene

Cited by 133 publications

References 36 publications

Genetic Contexts of bla NDM-1 in Patients Carrying Multiple NDM-Producing Strains

Genetic Contexts of bla NDM-1 in Patients Carrying Multiple NDM-Producing Strains

Colistin-Nonsusceptible Pseudomonas aeruginosa Sequence Type 654 with bla NDM-1 Arrives in North America

Salmonella Pathogenicity and Host Adaptation in Chicken-Associated Serovars

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Evolution of IncA/C bla _CMY-2 -Carrying Plasmids by Acquisition of the bla _NDM-1 Carbapenemase Gene

Genetic Contexts of bla _NDM-1 in Patients Carrying Multiple NDM-Producing Strains

Genetic Contexts of bla _NDM-1 in Patients Carrying Multiple NDM-Producing Strains

Colistin-Nonsusceptible Pseudomonas aeruginosa Sequence Type 654 with bla _NDM-1 Arrives in North America