Evolution of genes associated with gynoecium patterning and fruit development in Solanaceae

Ortiz-Ramírez, Clara Inés; Plata-Arboleda, Sayonara; Pabón‐Mora, Natalia

doi:10.1093/aob/mcy007

Cited by 30 publications

(48 citation statements)

References 101 publications

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“…The latter stage does not coincide with F2 sensu Ortíz‐Ramírez et al . (), given that in situ hybridization at F2 was problematic due to secondary metabolite accumulation in fleshy fruits and lignification in dry dehiscent fruits. In situ hybridization with sense probes for all tested genes yielded no signal (Figure S1).…”

Section: Resultsmentioning

confidence: 97%

“…As it has been previously described, fleshy fruits of C. annuum and S. lycopersicum are characterized by having both periclinal and anticlinal cell division and cuticle deposition (Pabón‐Mora and Litt, 2011; Ortíz‐Ramírez et al ., ). The C. annuum fruits exhibit also a clear differentiation of the two inner layers of the endocarp with respect to the rest of the pericarp.…”

Section: Resultsmentioning

confidence: 97%

“…To further investigate the spatial and temporal expression patterns of ALC and SPT in Solanaceae, RNA in situ hybridization was performed on floral buds at different developmental stages and in fruits right after fertilization (i.e. F1 sensu Ortíz‐Ramírez et al ., ), and at a later stage of development ranging between 3 and 4 days after fertilization of N. obtusifolia , C. annuum and S. lycopersicum . The latter stage does not coincide with F2 sensu Ortíz‐Ramírez et al .…”

Section: Resultsmentioning

confidence: 99%

“…IND genes are the result of a Brassicaceae‐specific duplication event and, thus, the Solanaceae have pre‐duplication genes more similar to HEC3 than to IND . Both Solanaceae and Brassicaceae have the same number of copies of ALC and SPT (Ortíz‐Ramírez et al ., ). However, changes in copy number and functional motifs within Solanaceae are hard to correlate to shifts in fruit type (dry/fleshy).…”

Section: Introductionmentioning

confidence: 97%

“…On the other hand, expression analyses done so far in four different Solanaceae species with different fruit types show opposite expression of SPT and ALC orthologs between dry‐ and fleshy‐fruited species during fruit maturation. While ALC genes are turned off in the dry dehiscent fruits of Brunfelsia australis during fruit maturation, SPT orthologs show this trend of decreasing expression levels in fleshy fruits of C. annuum and Solanum lycopersicum during maturation (Ortíz‐Ramírez et al ., ). Altogether, these data are insufficient to understand the functional contribution of the bHLH downstream effectors in different Solanaceae fruit types.…”

Section: Introductionmentioning

confidence: 99%

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Expression and function of the bHLH genes ALCATRAZ and SPATULA in selected Solanaceae species

et al. 2019

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Summary The genetic mechanisms underlying fruit development have been identified in Arabidopsis and have been comparatively studied in tomato as a representative of fleshy fruits. However, comparative expression and functional analyses on the bHLH genes downstream the genetic network, ALCATRAZ (ALC) and SPATULA (SPT), which are involved in the formation of the dehiscence zone in Arabidopsis, have not been functionally studied in the Solanaceae. Here, we perform detailed expression and functional studies of ALC/SPT homologs in Nicotiana obtusifolia with capsules, and in Capsicum annuum and Solanum lycopersicum with berries. In Solanaceae, ALC and SPT genes are expressed in leaves, and all floral organs, especially in petal margins, stamens and carpels; however, their expression changes during fruit maturation according to the fruit type. Functional analyses show that downregulation of ALC/SPT genes does not have an effect on gynoecium patterning; however, they have acquired opposite roles in petal expansion and have been co‐opted in leaf pigmentation in Solanaceae. In addition, ALC/SPT genes repress lignification in time and space during fruit development in Solanaceae. Altogether, some roles of ALC and SPT genes are different between Brassicaceae and Solanaceae; while the paralogs have undergone some subfunctionalization in the former they are mostly redundant in the latter.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Expression and function of the bHLH genes ALCATRAZ and SPATULA in selected Solanaceae species

et al. 2019

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Carpels

Smyth¹

2022

Encyclopedia of Life Sciences

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Repeated evolution of a morphological novelty: a phylogenetic analysis of the inflated fruiting calyx in the Physalideae tribe (Solanaceae)

Deanna

Larter

Barboza

et al. 2019

American J of Botany

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PREMISE OF THE STUDY:The evolution of novel fruit morphologies has been integral to the success of angiosperms. The inflated fruiting calyx, in which the balloon-like calyx swells to completely surround the fruit, has evolved repeatedly across angiosperms and is postulated to aid in protection and dispersal. We investigated the evolution of this trait in the tomatillos and their allies (Physalideae, Solanaceae). METHODS:The Physalideae phylogeny was estimated using four regions (ITS, LEAFY, trnL-F, waxy) with maximum likelihood (ML) and Bayesian inference. Under the best-fitting ML model of trait evolution, we estimated ancestral states along with the numbers of gains and losses of fruiting calyx accrescence and inflation with Bayesian stochastic mapping. Also, phylogenetic signal in calyx morphology was examined with two metrics (parsimony score and Fritz and Purvis's D).KEY RESULTS: Based on our well-resolved and densely sampled phylogeny, we infer that calyx evolution has proceeded in a stepwise and directional fashion, from non-accrescent to accrescent to inflated. In total, we inferred 24 gains of accrescence, 24 subsequent transitions to a fully inflated calyx, and only two reversals. Despite this lability, fruiting calyx accrescence and inflation showed strong phylogenetic signal. CONCLUSIONS:Our phylogeny greatly improves the resolution of Physalideae and highlights the need for taxonomic work. The comparative analyses reveal that the inflated fruiting calyx has evolved many times and that the trajectory toward this phenotype is generally stepwise and irreversible. These results provide a strong foundation for studying the genetic and developmental mechanisms responsible for the repeated origins of this charismatic fruit trait.

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Evolution of genes associated with gynoecium patterning and fruit development in Solanaceae

Cited by 30 publications

References 101 publications

Expression and function of the bHLH genes ALCATRAZ and SPATULA in selected Solanaceae species

Expression and function of the bHLH genes ALCATRAZ and SPATULA in selected Solanaceae species

Carpels

Repeated evolution of a morphological novelty: a phylogenetic analysis of the inflated fruiting calyx in the Physalideae tribe (Solanaceae)

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