Evolution of Electrospinning in Liver Tissue Engineering

Vasudevan, Ashwini; Tripathi, Dinesh Mani; Sundarrajan, Subramanian; Venugopal, Jayarama Reddy; Ramakrishna, Seeram; Kaur, Savneet

doi:10.3390/biomimetics7040149

Cited by 14 publications

(11 citation statements)

References 100 publications

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“…Such scaffolds have advantages for tissue engineering, such as promoting cell adhesion, proliferation, migration, and differentiation [ 124 ]. Many natural and synthetic polymers produce scaffolds in skin tissue engineering [ 125 , 126 , 127 ]. Scientists produced many biodegradable polymers to transport pharmaceuticals, macromolecules, cells, and enzymes into the body [ 34 ].…”

Section: Biodegradable and Nondegradable Polymers For Wound Healingmentioning

confidence: 99%

Combinational System of Lipid-Based Nanocarriers and Biodegradable Polymers for Wound Healing: An Updated Review

Far

Naimi-Jamal

Sedaghat

et al. 2023

JFB

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Skin wounds have imposed serious socioeconomic burdens on healthcare providers and patients. There are just more than 25,000 burn injury-related deaths reported each year. Conventional treatments do not often allow the re-establishment of the function of affected regions and structures, resulting in dehydration and wound infections. Many nanocarriers, such as lipid-based systems or biobased and biodegradable polymers and their associated platforms, are favorable in wound healing due to their ability to promote cell adhesion and migration, thus improving wound healing and reducing scarring. Hence, many researchers have focused on developing new wound dressings based on such compounds with desirable effects. However, when applied in wound healing, some problems occur, such as the high cost of public health, novel treatments emphasizing reduced healthcare costs, and increasing quality of treatment outcomes. The integrated hybrid systems of lipid-based nanocarriers (LNCs) and polymer-based systems can be promising as the solution for the above problems in the wound healing process. Furthermore, novel drug delivery systems showed more effective release of therapeutic agents, suitable mimicking of the physiological environment, and improvement in the function of the single system. This review highlights recent advances in lipid-based systems and the role of lipid-based carriers and biodegradable polymers in wound healing.

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Section: Biodegradable and Nondegradable Polymers For Wound Healingmentioning

confidence: 99%

Combinational System of Lipid-Based Nanocarriers and Biodegradable Polymers for Wound Healing: An Updated Review

Far

Naimi-Jamal

Sedaghat

et al. 2023

JFB

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“…PLA and PLLA also have had various attempts to support the viability and function of liver cells [ 33 , 94 ]. It has been reported that hepatocytes grow well when fetal liver cells are cultured on a 3D printed PLLA scaffold.…”

Section: Advantages Of 3d Cultures In Studies On the Liver Metabolismmentioning

confidence: 99%

Current Research Trends in the Application of In Vitro Three-Dimensional Models of Liver Cells

2022

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The liver produces and stores various nutrients that are necessary for the body and serves as a chemical plant, metabolizing carbohydrates, fats, hormones, vitamins, and minerals. It is also a vital organ for detoxifying drugs and exogenous harmful substances. Culturing liver cells in vitro under three-dimensional (3D) conditions is considered a primary mechanism for liver tissue engineering. The 3D cell culture system is designed to allow cells to interact in an artificially created environment and has the advantage of mimicking the physiological characteristics of cells in vivo. This system facilitates contact between the cells and the extracellular matrix. Several technically different approaches have been proposed, including bioreactors, chips, and plate-based systems in fluid or static media composed of chemically diverse materials. Compared to conventional two-dimensional monolayer culture in vitro models, the ability to predict the function of the tissues, including the drug metabolism and chemical toxicity, has been enhanced by developing three-dimensional liver culture models. This review discussed the methodology of 3D cell cultures and summarized the advantages of an in vitro liver platform using 3D culture technology.

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“…Electrospinning is the most appropriate technique to fabricate nanoscaffolds because nanofibers produced as a result of electrospinning closely mimic the dense collagen network of the tissue ECM . Electrospinning also offers user-defined scaffold fabrication with tunable pore sizes. , Nanofiber scaffolds provide a high surface-to-volume ratio, with an ability to incorporate more cells in limited space . The diameter of nanofiber scaffolds provides an optimum spatial arrangement suitable for integrin binding of the cells .…”

Section: Introductionmentioning

confidence: 99%

Liver Extracellular Matrix-Based Nanofiber Scaffolds for the Culture of Primary Hepatocytes and Drug Screening

Vasudevan,

Majumder,

Sharma

et al. 2023

ACS Biomater. Sci. Eng.

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Immortalized liver cell lines and primary hepatocytes are currently used as in vitro models for hepatotoxic drug screening. However, a decline in the viability and functionality of hepatocytes with time is an important limitation of these culture models. Advancements in tissue engineering techniques have allowed us to overcome this challenge by designing suitable scaffolds for maintaining viable and functional primary hepatocytes for a longer period of time in culture. In the current study, we fabricated liverspecific nanofiber scaffolds with polylactic acid (PLA) along with a decellularized liver extracellular matrix (LEM) by the electrospinning technique. The fabricated hybrid PLA-LEM scaffolds were more hydrophilic and had better swelling properties than the PLA scaffolds. The hybrid scaffolds had a pore size of 38 ± 8 μm and supported primary rat hepatocyte cultures for 10 days. Increased viability (2fold increase in the number of live cells) and functionality (5-fold increase in albumin secretion) were observed in primary hepatocytes cultured on the PLA-LEM scaffolds as compared to those on conventional collagen-coated plates on day 10 of culture. A significant increase in CYP1A2 enzyme activity was observed in hepatocytes cultured on PLA-LEM hybrid scaffolds in comparison to those on collagen upon induction with phenobarbital. Drugs like acetaminophen and rifampicin showed the highest toxicity in hepatocytes cultured on hybrid scaffolds. Also, the lethal dose of these drugs in rodents was accurately predicted as 1.6 g/kg and 594 mg/kg, respectively, from the corresponding IC 50 values obtained from drug-treated hepatocytes on hybrid scaffolds. Thus, the fabricated liver-specific electrospun scaffolds maintained primary hepatocyte viability and functionality for an extended period in culture and served as an effective ex vivo drug screening platform to predict an accurate in vivo drug-induced hepatotoxicity.

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Evolution of Electrospinning in Liver Tissue Engineering

Cited by 14 publications

References 100 publications

Combinational System of Lipid-Based Nanocarriers and Biodegradable Polymers for Wound Healing: An Updated Review

Combinational System of Lipid-Based Nanocarriers and Biodegradable Polymers for Wound Healing: An Updated Review

Current Research Trends in the Application of In Vitro Three-Dimensional Models of Liver Cells

Liver Extracellular Matrix-Based Nanofiber Scaffolds for the Culture of Primary Hepatocytes and Drug Screening

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