Evolution of Bunyaviruses by Genome Reassortment in Dually Infected Mosquitoes (
            <i>Aedes triseriatus</i>
            )

Beaty, Barry J.; Sundin, Daniel R.; Chandler, Laura J.; Bishop, David H. L.

doi:10.1126/science.4048949

Cited by 104 publications

(67 citation statements)

References 17 publications

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“…Genome recruitment is expected to be similar in all phleboviruses, thus the conserved hydrophobic pocket of N is a candidate G N binding site. This hypothesis is consistent with the ability of bunyaviruses, both in nature and in vitro, to undergo reassortment in which progeny have genomic segments that derive from more than one parental virus (32)(33)(34). Reassortment requires promiscuity in the interaction of N with genomic RNAs from heterologous viruses and in protein-protein interactions necessary for assembling virions.…”

Section: Discussionsupporting

confidence: 64%

Structure of the Rift Valley fever virus nucleocapsid protein reveals another architecture for RNA encapsidation

Raymond¹,

Piper²,

Gerrard

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

115

144

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Rift Valley fever virus (RVFV) is a negative-sense RNA virus (genus Phlebovirus, family Bunyaviridae) that infects livestock and humans and is endemic to sub-Saharan Africa. Like all negative-sense viruses, the segmented RNA genome of RVFV is encapsidated by a nucleocapsid protein (N). The 1.93-Å crystal structure of RVFV N and electron micrographs of ribonucleoprotein (RNP) reveal an encapsidated genome of substantially different organization than in other negative-sense RNA virus families. The RNP polymer, viewed in electron micrographs of both virus RNP and RNP reconstituted from purified N with a defined RNA, has an extended structure without helical symmetry. N-RNA species of ∼100-kDa apparent molecular weight and heterogeneous composition were obtained by exhaustive ribonuclease treatment of virus RNP, by recombinant expression of N, and by reconstitution from purified N and an RNA oligomer. RNA-free N, obtained by denaturation and refolding, has a novel all-helical fold that is compact and well ordered at both the N and C termini. Unlike N of other negative-sense RNA viruses, RVFV N has no positively charged surface cleft for RNA binding and no protruding termini or loops to stabilize a defined N-RNA oligomer or RNP helix. A potential protein interaction site was identified in a conserved hydrophobic pocket. The nonhelical appearance of phlebovirus RNP, the heterogeneous ∼100-kDa N-RNA multimer, and the N fold differ substantially from the RNP and N of other negative-sense RNA virus families and provide valuable insights into the structure of the encapsidated phlebovirus genome.ribonucleoprotein | viral protein | segmented genome | negative-sense RNA virus

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Section: Discussionsupporting

confidence: 64%

Structure of the Rift Valley fever virus nucleocapsid protein reveals another architecture for RNA encapsidation

Raymond¹,

Piper²,

Gerrard

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

115

144

View full text Add to dashboard Cite

show abstract

“…Reassortment of other bunyaviruses has been demonstrated in vitro in both mosquito and mammalian cells, and in vivo in mosquitoes that are dually infected. 24,30,32,33 In addition, analysis of field isolates suggests that reassortment also can occur in nature. [34][35][36] However, most of these reassortants had been limited to closely related bunyaviruses within a subgroup of the same serogroup, except one report with limited laboratory generated reassortant genotypes between viruses from California encephalitis (CE) subgroup and trivittatus (TVT).…”

Section: Discussionmentioning

confidence: 99%

“…Studies have demonstrated the potential for superinfection in mosquitoes when the period between initial and subsequent infective blood meals is less than 48 hr. 30 In addition, feeding by some mosquito species in nature is easily interrupted by host defensive behavior, which can result in an individual mosquito taking blood meals from more than one viremic host. 31 The opportunity for dual bunyavirus infection to occur in Ae.…”

Section: Discussionmentioning

confidence: 99%

Potential for evolution of California serogroup bunyaviruses by genome reassortment in Aedes albopictus.

Cheng¹,

Rodas²,

Schultz³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

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Abstract. Aedes albopictus was introduced into the United States in used tires in 1985. Its successful colonization of the upper Midwest has potential to alter the current epidemiology of bunyaviruses that circulate in the region. It is permissive for the replication of several arboviruses, including La Crosse (LACV) and Jamestown Canyon (JCV) bunyaviruses. In this study, we demonstrate the ability of LACV and JCV to coinfect Ae. albopictus mosquitoes and to form all six possible reassortant genotypes. All reassortant viruses infect Ae. albopictus orally and can be transmitted to suckling mice. All reassortants are neurovirulent in mice. However, reassortant viruses carrying the LACV M segment in the foreign genetic background of JCV are more neuroinvasive than JCV, or any other reassortant genotype. In addition, these reassortants can replicate in gerbils and infect Ae. triseriatus, characteristics of LACV, but not JCV. Because Ae. albopictus is spreading into new geographic areas and feeds on a variety of mammals, including humans, it has the potential to transmit new, emerging bunyaviruses in nature.

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“…Consequently, it is necessary to determine the extent to which reassortment can occur in vivo. Reassortment of an arbovirus (La Crosse bunyavirus) has been demonstrated in its arthropod vector (Aedes triseriatus) but attempts to duplicate the results in vertebrates were unsuccessful (Beaty et al, 1985). Since vertebrates often act as amplifying hosts, providing a source of virus for countless vectors, the vertebrate host is potentially an important site of reassortant virus formation.…”

Section: Introductionmentioning

confidence: 99%

Reassortment of Thogoto Virus (a Tick-borne Influenza-like Virus) in a Vertebrate Host

Jones

Davies

Green

et al. 1987

Journal of General Virology

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Evolution of Bunyaviruses by Genome Reassortment in Dually Infected Mosquitoes ( Aedes triseriatus )

Cited by 104 publications

References 17 publications

Structure of the Rift Valley fever virus nucleocapsid protein reveals another architecture for RNA encapsidation

Structure of the Rift Valley fever virus nucleocapsid protein reveals another architecture for RNA encapsidation

Potential for evolution of California serogroup bunyaviruses by genome reassortment in Aedes albopictus.

Reassortment of Thogoto Virus (a Tick-borne Influenza-like Virus) in a Vertebrate Host

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