2014
DOI: 10.3892/or.2014.3411
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Evolution of a gastric carcinoma cell-specific DNA aptamer by live cell-SELEX

Abstract: Aptamers have emerged as promising molecular probes for disease diagnosis and therapy. In the present study, the entire live cell-SELEX method was used to generate gastric cancer cell‑specific aptamers. Human gastric carcinoma AGS cells were used as target cells for positive selections and human normal gastric epithelial GES-1 cells as the negative cells for counter selections. The selection procedure was monitored by gel electrophoresis and flow cytometric analysis. By successive in vitro evolutions and subse… Show more

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Cited by 18 publications
(16 citation statements)
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“…Consequently, scientists have been working hard on different variants of aptamer selection methodology. The advances and improvements made in traditional SELEX have allowed the development of new methods that permit the selection of aptamers against not only proteins [ 23 ] but also against a wide range of targets, such as live cells [ 24 , 25 , 26 , 27 ], viruses [ 28 ], bacteria [ 29 ], and tissues [ 30 ]. Cell-based SELEX methodology (cell-SELEX) stands out among other new aptamer-selection methods: the oligonucleotides selected with this technique are able to bind not just cell-surface molecules in their native state, but also unknown membrane receptors [ 31 , 32 ].…”
Section: Aptamersmentioning
confidence: 99%
“…Consequently, scientists have been working hard on different variants of aptamer selection methodology. The advances and improvements made in traditional SELEX have allowed the development of new methods that permit the selection of aptamers against not only proteins [ 23 ] but also against a wide range of targets, such as live cells [ 24 , 25 , 26 , 27 ], viruses [ 28 ], bacteria [ 29 ], and tissues [ 30 ]. Cell-based SELEX methodology (cell-SELEX) stands out among other new aptamer-selection methods: the oligonucleotides selected with this technique are able to bind not just cell-surface molecules in their native state, but also unknown membrane receptors [ 31 , 32 ].…”
Section: Aptamersmentioning
confidence: 99%
“…Currently, most cancers lack effective non-invasive screening tests and are therefore detected too late. Liquid biopsy is a promising tool for the early detection of cancer [ 3 , 4 ], and significant advancement of the technique shows potential as a diagnostic tool for a variety of cancer types, including melanoma [ 5 , 6 , 7 , 8 ], breast [ 9 , 10 , 11 ], colorectal [ 12 , 13 , 14 ], lung [ 15 ], liver [ 16 , 17 , 18 ], ovarian [ 19 ], pancreatic [ 20 ], and gastric cancers [ 21 ]. The most common analytical targets of liquid biopsy are circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), microRNA, and extracellular vesicles (EVs) [ 22 , 23 , 24 , 25 , 26 ].…”
Section: Introductionmentioning
confidence: 99%
“…SELEX has been successfully integrated in microfluidic chip based systems to screen aptamers against the protein targets with high affinities [ 11 18 ]. Moreover, Cell SELEX explores the expression of cell surface epitopes and distinguishes between different types of target cells [ 19 22 ]. High-throughput next-generation sequencing (NGS) and subsequent computational data analysis have also been integrated in SELEX to optimize aptamer discovery at a high resolution[ 11 ].…”
Section: Introductionmentioning
confidence: 99%
“…Moreover, two DNA aptamers bound to lgV-like N domain of CEA were found inhibiting cell adhesion properties of cancer cells [ 29 ]. DNA aptamer cy-apt20 was found targeting human gastric carcinoma AGS cells while showed minimal recognition to normal gastric epithelial GES-1 cells [ 22 ]. Eight colorectal cancer stem cells (CR-CSCs)/CRC-specific aptamers were reported and three of them showed high affinities towards their respective target cells [ 20 ].…”
Section: Introductionmentioning
confidence: 99%