Evolution of 17-β-estradiol, estrone and estrone-sulfate concentrations in late pregnancy of different breeds of mares using Liquid Chromatography and Mass Spectrometry

Ledeck, Joy; Dufour, Patrice; Evrard, Élise; Goff, Caroline Le; Peeters, Stéphanie; Brutinel, Flore; Egyptien, Sophie; Deleuze, Stéfan; Cavalier, Étienne; Ponthier, Jérôme

doi:10.1016/j.theriogenology.2022.06.004

Cited by 4 publications

(2 citation statements)

References 27 publications

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“…Furthermore, circulating pregnancy hormone concentrations differ in the two donor species compared to mice. For example, the serum concentration of 17βestradiol (E2), a potent estrogen that promotes post-natal mammary growth, increases during pregnancy [77,78] and rises to ~35 pg/ml in dogs and ~50 pg/ml in horses at 2 weeks and 5 months gestation, respectively [79][80][81], but rises to a substantially greater concentration of 1000-1800 pg/ml in mice at 7 days gestation [68]. Interestingly, administration of E2 in cows promotes mammary involution and decreases milk synthesis [82], indicating that high E2 levels within host mice may have potentially had an inhibitory effect on the pregnancy-associated phenotypes in the equine xenografts.…”

Section: Plos Onementioning

confidence: 99%

A xenotransplantation mouse model to study physiology of the mammary gland from large mammals

Miller,

Reddy,

Harman

et al. 2024

PLoS ONE

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Although highly conserved in structure and function, many (patho)physiological processes of the mammary gland vary drastically between mammals, with mechanisms regulating these differences not well understood. Large mammals display variable lactation strategies and mammary cancer incidence, however, research into these variations is often limited to in vitro analysis due to logistical limitations. Validating a model with functional mammary xenografts from cryopreserved tissue fragments would allow for in vivo comparative analysis of mammary glands from large and/or rare mammals and would improve our understanding of postnatal development, lactation, and premalignancy across mammals. To this end, we generated functional mammary xenografts using mammary tissue fragments containing mammary stroma and parenchyma isolated via an antibody-independent approach from healthy, nulliparous equine and canine donor tissues to study these species in vivo. Cryopreserved mammary tissue fragments were xenotransplanted into de-epithelialized fat pads of immunodeficient mice and resulting xenografts were structurally and functionally assessed. Preimplantation of mammary stromal fibroblasts was performed to promote ductal morphogenesis. Xenografts recapitulated mammary lobule architecture and contained donor-derived stromal components. Mammatropic hormone stimulation resulted in (i) upregulation of lactation-associated genes, (ii) altered proliferation index, and (iii) morphological changes, indicating functionality. Preimplantation of mammary stromal fibroblasts did not promote ductal morphogenesis. This model presents the opportunity to study novel mechanisms regulating unique lactation strategies and mammary cancer induction in vivo. Due to the universal applicability of this approach, this model serves as proof-of-concept for developing mammary xenografts for in vivo analysis of virtually any mammals, including large and rare mammals.

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Section: Plos Onementioning

confidence: 99%

A xenotransplantation mouse model to study physiology of the mammary gland from large mammals

Miller,

Reddy,

Harman

et al. 2024

PLoS ONE

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“…For example, progesterone (PROG) and 17b-estradiol (17b-E2) measurements in plasma of cyclic mares by enzyme immunoassay and radioimmunoassay, respectively, showed that concentrations of PROG begin to increase the day of ovulation, reach maximal concentrations at 6 to 8 days post-ovulation and decrease until day 14 post-ovulation whereas concentrations of 17b-E2 reach a peak 2 days before ovulation and then decrease (Ginther et al, 2005;Aurich, 2011). Gas chromatography coupled to mass spectrometry (GC-MS), liquid chromatography coupled to mass spectrometry (LC-MS) and tandem mass spectrometry (LC-MS/ MS) analysis have been developed to measure some pregnanes (C21-steroids), androstanes (C19-steroids) and estranes (C18steroids) in serum of male and female uncharacterized horses (Genangeli et al, 2017;Dufour et al, 2021) and in plasma throughout equine gestation (Scholtz et al, 2014;Legacki et al, 2016;Wynn et al, 2018a;Ledeck et al, 2022). Analysis in pregnant mares showed that plasmatic PROG concentrations were high during the first 12 weeks of pregnancy and declined progressively until undetectable levels by day 200.…”

Section: Introductionmentioning

confidence: 99%

Saliva and plasma steroidome in mare during reproductive stages: A GC-MS/MS study

et al. 2022

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Precision livestock farming using omics approach to acquire precise and real-time data can help farmers in individual animal management and decision making. Since steroid hormones play a key role for the regulation of reproductive functions, reproduction management could be improved by characterizing the steroidome during reproductive stages. Moreover, saliva collection is a non-invasive, painless, inexpensive and easy sampling method. Thus, this prospective study proposes a steroidomic analysis in mare saliva during reproductive stages, that could help to identify potential biomarkers to accurately detect their reproductive stage in a welfare friendly production system, for real-time decision making at the individual animal level. Correlation between saliva and plasma steroidome was also investigated. Saliva and blood samples from 6 mares were collected in anestrus, in the follicular phase 3 days, 2 days and 1 day before ovulation and the day when ovulation was detected, in the luteal phase 6 days after ovulation and in gestation 18 days after ovulation and insemination. Steroidome analysis was performed by gas chromatography coupled to tandem mass spectrometry (GC-MS/MS). We characterized 25 and 36 steroids in saliva and plasma respectively. Pregnenolone concentrations in saliva during gestation were significantly higher than during anestrus or follicular phase and tended to be higher than during luteal phase. Most of the 5α-reduced metabolites of progesterone showed higher salivary concentrations during the luteal phase 6 days post-ovulation and during gestation 18 days post-ovulation compared to anestrus and follicular phase. These steroids could be potential salivary biomarkers of the reproductive stage of the mare. Further studies with a greater number of animals are in progress to confirm the reliability of these potential candidate biomarkers and to develop field-friendly assays.

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Analysis of progesterone and estrone-sulfate in feces of American Bison using liquid chromatography coupled to mass spectrometry: Technical validation and correlation with blood levels

Dufour,

Frisée,

Rigaux

et al. 2024

Domestic Animal Endocrinology

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Evolution of 17-β-estradiol, estrone and estrone-sulfate concentrations in late pregnancy of different breeds of mares using Liquid Chromatography and Mass Spectrometry

Cited by 4 publications

References 27 publications

A xenotransplantation mouse model to study physiology of the mammary gland from large mammals

A xenotransplantation mouse model to study physiology of the mammary gland from large mammals

Saliva and plasma steroidome in mare during reproductive stages: A GC-MS/MS study

Analysis of progesterone and estrone-sulfate in feces of American Bison using liquid chromatography coupled to mass spectrometry: Technical validation and correlation with blood levels

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