Evolution and Structure of API5 and Its Roles in Anti-Apoptosis

Chen, Meishan; Wu, Weiwei; Liu, Dongwu; Lv, Yanhua; Deng, Hongkuan; Gao, Sijia; Gu, Yaqi; Huang, Mujie; Guo, Xiao; Liu, Baohua; Zhao, Baisuo; Pang, Qiuxiang

doi:10.2174/0929866527999201211195551

Cited by 9 publications

(7 citation statements)

References 79 publications

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“…We found that the proteins that may interact with these two proteins are involved in physiological activities such as transcriptional, post-translational regulation and apoptosis. For example, the Api5 (apoptosis inhibitor 5) participates in cell apoptosis as an effective inhibitor ( Chen et al, 2021 ), whereas the Sap18 and Pabpn are involved in the post-transcriptional and post-translational modification processes, respectively ( Nicholson & Pasquinelli, 2019 ; Zhang et al, 1997 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of quantitative phosphoproteomics between two tilapias (Oreochromis niloticus and Oreochromis aureus) under low-temperature stress

Yang

Fan

et al. 2023

PeerJ

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As an important farmed fish, tilapia has poor tolerance to low-temperatures. At the same time, different tilapia strains have apparent differences in low-temperature tolerance. In this study, using the iTRAQ method, the phosphorylated proteomics of two tilapia strains (Oreochromis niloticus and Oreochromis aureus) with different tolerances to low-temperature stress were quantitatively and comparatively analyzed, to clarify the physiological mechanism of tilapia’s response to low-temperature stress. Through the GO and IPR analyses of differentially phosphorylated proteins, a number of similarities in physiological activities and regulatory effects were found between the two tilapias in response to low-temperature stress. Many differentially phosphorylated proteins are mainly involved in lipid metabolism, cell proliferation and apoptosis. However, the difference in endurance of low temperature of these two tilapias might be related to the differences in categories, expression and modification level of genetic products which were involved in the aforementioned physiological processes. And meanwhile, the enrichment results of KEGG showed the changes of multiple immune-related and growth-related phosphorylated proteins in the cytokine-cytokine receptor interaction pathway in O. aureus are more prominent. Furthermore, the significantly enriched pathway of carbohydrate digestion and absorption in O. niloticus may indicate that low-temperature stress exerts a more severe impact on energy metabolism. The relative results would help elucidating the molecular mechanism by which tilapia responds to low-temperature stress, and developing culture of tilapia species.

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Section: Discussionmentioning

confidence: 99%

Comparative analysis of quantitative phosphoproteomics between two tilapias (Oreochromis niloticus and Oreochromis aureus) under low-temperature stress

Yang

Fan

et al. 2023

PeerJ

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“…Apoptosis, also known as programmed cell death, is a physiological process that occurs in multicellular organisms ( 30 ). Chen et al ( 31 ) demonstrate that apoptosis acts as a critical participant in numerous biological processes, including tissue development and organ formation. Downregulation of GINS2 induces cell cycle arrest and apoptosis in lung cancer A549 cells ( 32 ).…”

Section: Discussionmentioning

confidence: 99%

GINS2 regulates the proliferation and apoptosis of colon cancer cells through PTP4A1

Zhe

2022

Mol Med Rep

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colon cancer is associated with high death rates worldwide and poses a serious threat to public health. GinS complex subunit 2 (GinS2) serves a carcinogenic role in many cancers, including gastric adenocarcinoma, ovarian cancer and pancreatic cancer. However, the specific function of GINS2 in the development of colon cancer has not been described in detail. The present study aimed to clarify the role of GINS2 in colon cancer. a cell counting Kit-8 assay, edu staining, TUNEL and flow cytometry analyses were performed to determine the levels of cell viability, proliferation and apoptosis and to evaluate the cell cycle. Through the analysis of BioGrid, a Protein-Protein interaction database, it was hypothesized that protein tyrosine phosphatase 4a1 (PTP4a1) is a protein that might interact with GinS2, which was then validated using a co-immunoprecipitation assay. mrna and protein levels were measured using reverse transcription-quantitative Pcr and western blotting, respectively. The results of the present study demonstrated that GinS2 expression levels were increased in colon cancer cells. Furthermore, GinS2 knockdown inhibited the proliferation of colon cancer cells, while the levels of cell cycle arrest and apoptosis were increased. By interacting with PTP4A1, GINS2 promoted the expression of PTP4A1, a novel p53 target. GinS2 knockdown was increased, while PTP4a1 overexpression decreased the protein level of p53. Notably, PTP4A1 overexpression partly reversed the effects of GINS2 downregulation on colon cancer cells. Therefore, the present study demonstrated that GINS2 regulated the proliferation and apoptosis of colon cancer cells through PTP4A1/p53 pathway, highlighting that GinS2 may serve as a novel molecular marker for colon cancer prevention and therapy.

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“…It belongs to the API5 family, whose members contain the so-called API5 domain (Pfam ID PF05918), a domain notably absent in proteins encoded by eubacteria, archaebacteria, and viruses' genomes, but which is encoded by genomes from several ancient eukaryotic taxonomic groups [37]. Alignment analyses of API5 orthologs across species, vertebrates, and invertebrates, indicate a high level of conservation in the sequence and protein domains [38][39][40]. It is well established that protein domains are functional and structural units within proteins that can be added, rearranged, or combined through evolution to create novel functionalities and facilitate the creation of complex biological systems [41][42][43].…”

Section: Structure and Binding Partners Of Api5mentioning

confidence: 99%

Apoptosis Inhibitor 5: A Multifaceted Regulator of Cell Fate

Abbas,

Derkaoui,

Jeammet

et al. 2024

Biomolecules

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Apoptosis, or programmed cell death, is a fundamental process that maintains tissue homeostasis, eliminates damaged or infected cells, and plays a crucial role in various biological phenomena. The deregulation of apoptosis is involved in many human diseases, including cancer. One of the emerging players in the intricate regulatory network of apoptosis is apoptosis inhibitor 5 (API5), also called AAC-11 (anti-apoptosis clone 11) or FIF (fibroblast growth factor-2 interacting factor). While it may not have yet the same level of notoriety as some other cancer-associated proteins, API5 has garnered increasing attention in the cancer field in recent years, as elevated API5 levels are often associated with aggressive tumor behavior, resistance to therapy, and poor patient prognosis. This review aims to shed light on the multifaceted functions and regulatory mechanisms of API5 in cell fate decisions as well as its interest as therapeutic target in cancer.

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Evolution and Structure of API5 and Its Roles in Anti-Apoptosis

Cited by 9 publications

References 79 publications

Comparative analysis of quantitative phosphoproteomics between two tilapias (Oreochromis niloticus and Oreochromis aureus) under low-temperature stress

Comparative analysis of quantitative phosphoproteomics between two tilapias (Oreochromis niloticus and Oreochromis aureus) under low-temperature stress

GINS2 regulates the proliferation and apoptosis of colon cancer cells through PTP4A1

Apoptosis Inhibitor 5: A Multifaceted Regulator of Cell Fate

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