Evolution and Structure of API5 and Its Roles in Anti-Apoptosis

Chen, Meishan; Wu, Weiwei; Liu, Dongwu; Lv, Yanhua; Deng, Hongkuan; Gao, Sijia; Gu, Yaqi; Huang, Mujie; Guo, Xiao; Liu, Baohua; Zhao, Baisuo; Pang, Qiuxiang

doi:10.2174/0929866527999201211195551

Cited by 9 publications

(2 citation statements)

References 79 publications

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“…Apoptosis, also known as programmed cell death, is a physiological process that occurs in multicellular organisms ( 30 ). Chen et al ( 31 ) demonstrate that apoptosis acts as a critical participant in numerous biological processes, including tissue development and organ formation. Downregulation of GINS2 induces cell cycle arrest and apoptosis in lung cancer A549 cells ( 32 ).…”

Section: Discussionmentioning

confidence: 99%

GINS2 regulates the proliferation and apoptosis of colon cancer cells through PTP4A1

Zhe

2022

Mol Med Rep

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colon cancer is associated with high death rates worldwide and poses a serious threat to public health. GinS complex subunit 2 (GinS2) serves a carcinogenic role in many cancers, including gastric adenocarcinoma, ovarian cancer and pancreatic cancer. However, the specific function of GINS2 in the development of colon cancer has not been described in detail. The present study aimed to clarify the role of GINS2 in colon cancer. a cell counting Kit-8 assay, edu staining, TUNEL and flow cytometry analyses were performed to determine the levels of cell viability, proliferation and apoptosis and to evaluate the cell cycle. Through the analysis of BioGrid, a Protein-Protein interaction database, it was hypothesized that protein tyrosine phosphatase 4a1 (PTP4a1) is a protein that might interact with GinS2, which was then validated using a co-immunoprecipitation assay. mrna and protein levels were measured using reverse transcription-quantitative Pcr and western blotting, respectively. The results of the present study demonstrated that GinS2 expression levels were increased in colon cancer cells. Furthermore, GinS2 knockdown inhibited the proliferation of colon cancer cells, while the levels of cell cycle arrest and apoptosis were increased. By interacting with PTP4A1, GINS2 promoted the expression of PTP4A1, a novel p53 target. GinS2 knockdown was increased, while PTP4a1 overexpression decreased the protein level of p53. Notably, PTP4A1 overexpression partly reversed the effects of GINS2 downregulation on colon cancer cells. Therefore, the present study demonstrated that GINS2 regulated the proliferation and apoptosis of colon cancer cells through PTP4A1/p53 pathway, highlighting that GinS2 may serve as a novel molecular marker for colon cancer prevention and therapy.

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Section: Discussionmentioning

confidence: 99%

GINS2 regulates the proliferation and apoptosis of colon cancer cells through PTP4A1

Zhe

2022

Mol Med Rep

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“…Anti-apoptosis clone-11 protein (AAC-11) is an apoptosis-inhibiting nuclear protein highly expressed in various cancer cells and tissues, this overexpression being associated with poor prognosis [ 183 , 184 , 185 ]. Recent studies revealed that AAC-11 expression correlates with anticancer drug resistance and contributes to tumor invasion and metastasis [ 186 , 187 , 188 , 189 ].…”

Section: Team Workmentioning

confidence: 99%

Keeping Cell Death Alive: An Introduction into the French Cell Death Research Network

et al. 2022

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Since the Nobel Prize award more than twenty years ago for discovering the core apoptotic pathway in C. elegans, apoptosis and various other forms of regulated cell death have been thoroughly characterized by researchers around the world. Although many aspects of regulated cell death still remain to be elucidated in specific cell subtypes and disease conditions, many predicted that research into cell death was inexorably reaching a plateau. However, this was not the case since the last decade saw a multitude of cell death modalities being described, while harnessing their therapeutic potential reached clinical use in certain cases. In line with keeping research into cell death alive, francophone researchers from several institutions in France and Belgium established the French Cell Death Research Network (FCDRN). The research conducted by FCDRN is at the leading edge of emerging topics such as non-apoptotic functions of apoptotic effectors, paracrine effects of cell death, novel canonical and non-canonical mechanisms to induce apoptosis in cell death-resistant cancer cells or regulated forms of necrosis and the associated immunogenic response. Collectively, these various lines of research all emerged from the study of apoptosis and in the next few years will increase the mechanistic knowledge into regulated cell death and how to harness it for therapy.

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Astragaloside IV: A promising natural neuroprotective agent for neurological disorders

Yang¹,

Zhang²,

Wang³

2023

Biomedicine & Pharmacotherapy

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Evolution and Structure of API5 and Its Roles in Anti-Apoptosis

Cited by 9 publications

References 79 publications

GINS2 regulates the proliferation and apoptosis of colon cancer cells through PTP4A1

GINS2 regulates the proliferation and apoptosis of colon cancer cells through PTP4A1

Keeping Cell Death Alive: An Introduction into the French Cell Death Research Network

Astragaloside IV: A promising natural neuroprotective agent for neurological disorders

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